Structural biology of the selenocysteine synthesis system.

• Project/Area Number: 20570148
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: The University of Tokyo
• Principal Investigator: SEKINE Shun-ichi 東京大学, 大学院・理学系研究科, 特任准教授 (50321774)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: セレノシステイン / セレン / 翻訳 / tRNA / サプレッサー / X線結晶構造解析 / 翻訳延長因子 / セレノリン酸 / オパール / 翻訳伸長因子

Research Abstract

Selenium is an essential micronutrient for many organisms, including humans. It is present in proteins as a selenocysteine(Sec) residue(s), which is known as the 21^(st) amino acid that is translationally incorporated into proteins. To elucidate structural basis of selenocysteine biosynthesis, we determined 3D structures of these proteins and tRNA by X-ray crystallography.

Research Products

• [Journal Article] セレンをタンパク質に正確に取り込む:セレノシステインtRNA認識の構造基盤 (2011)
  • Author(s): 関根俊一, 横山茂之
  • Journal Title: 生物物理 Volume: 51(6) Pages: 272-273
  • NAID: 10030038012
• [Journal Article] Structural basis for the major role of O-phosphoseryl-tRNA kinase in the UGA-specific encoding of selenocysteine. (2010)
  • Author(s): Chiba, S., Itoh, Y., Sekine, S. and Yokoyama, S.
  • Journal Title: Mol. Cell Volume: 39 Pages: 410-420
• [Journal Article] Structural basis for the major role of O-phosphoseryl-tRNA kinase in the UGA-specific encoding of selenocysteine. (2010)
  • Author(s): Chiba, S., et al.
  • Journal Title: Molecular Cell Volume: 39 Pages: 410-420
  • Peer Reviewed
• [Journal Article] Crystal structure of human selenocysteine tRNA. (2009)
  • Author(s): Itoh, Y., Chiba, S., Sekine, S. and Yokoyama S.
  • Journal Title: Nucleic Acids Res Volume: 37 Pages: 6259-6268
• [Journal Article] Structure of selenophosphate synthetase essential for selenium incorporation into proteins and RNAs. (2009)
  • Author(s): Itoh, Y., Sekine, S., Matsumoto, E., Akasaka, R., Takemoto, C., Shirouzu, M. and Yokoyama, S.
  • Journal Title: J. Mol. Biol Volume: 385 Pages: 1456-1469
• [Journal Article] Crystal structure of human selenocysteine tRNA. (2009)
  • Author(s): Itoh Y, Chiba S, Sekine S, Yokoyama S.
  • Journal Title: Nucleic Acids Research 37 Pages: 6259-6268
  • Peer Reviewed
• [Journal Article] Crystallographic and mutational studies of seryl-tRNA synthetase from the archaeon Pyrococcus horikoshii. (2008)
  • Author(s): Itoh, Y., Sekine, S., Kuroishi, C., Terada, T., Shirouzu, M., Kuramitsu, S. and Yokoyama, S.
  • Journal Title: RNA Biol Volume: 5 Pages: 169-177
• [Journal Article] Structure of an N-terminally truncated selenophosphate synthetase from Aquifex aeolicus. (2008)
  • Author(s): Matsumoto, E., Sekine, S., Akasaka, R., Otta, Y., Katsura, K., Inoue, M., Kaminishi, T., Terada, T., Shirouzu, M. and Yokoyama, S.
  • Journal Title: Acta Crystallogr Volume: F64 Pages: 453-458
• [Presentation] O-ホスホセリルtRNAキナーゼとセレノシステインtRNA複合体の結晶構造 (2010)
  • Author(s): 伊藤弓弦, 他
  • Organizer: 第12回RNAミーティング
  • Place of Presentation: 一橋記念講堂(東京)
• [Presentation] セレノシステイン合成酵素の結晶構造解析 (2009)
  • Author(s): 伊藤弓弦、関根俊一、横山茂之
  • Organizer: 第11回RNAミーティング
  • Place of Presentation: 新潟
• [Presentation] ヒトのセレノシステインtRNAの結晶構造解析 (2009)
  • Author(s): 伊藤弓弦、千葉志穂、関根俊一、横山茂之
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] セレノシステイン合成酵素の結晶構造解析 (2009)
  • Author(s): 伊藤弓弦、関根俊一、横山茂之
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] セレノリン酸合成酵素SPSの結晶構造 (2008)
  • Author(s): 関根俊一
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2008-12-09
• [Presentation] セレノリン酸合成酵素の構造解析 (2008)
  • Author(s): 伊藤弓弦
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2008-12-09
• [Presentation] Implications for selenophosphate generation by crystal structure of selenophosphate synthetase (2008)
  • Author(s): 関根俊一
  • Organizer: XXI Congress of the international Union of Crystallography (IUCr2008)
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2008-08-23
• [Presentation] Implications for selenophosphate generation by crystal structure of selenophosphate synthetase (2008)
  • Author(s): Sekine, S., et al.
  • Organizer: IUCR 2008
  • Place of Presentation: Osaka
• [Remarks]
  • URL: http://www.s.u-tokyo.ac.jp/press/press-2010-34.html
• [Remarks]
  • URL: http://www.s.u-tokyo.ac.jp/press/press-2010-34.html
• [Remarks]
  • URL: http://www.riken.go.jp/r-world/info/release/press/2010/100813/detail.html