Actin filaments function as a tension sensor via tension dependent binding of cofilin to the filament
| Project/Area Number |
20570151
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Nagoya University |
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Principal Investigator |
TATSUMI Hitoshi Nagoya University, 医学系研究科, 准教授 (20171720)
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| Co-Investigator(Kenkyū-buntansha) |
SOKABE Masahiro 名古屋大学, 大学院・医学系研究科, 教授 (10093428)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | コフィリン / アクチン / 一分子測定 / ねじれ / 光ピンセット / 機械刺激 / 張力受容 / 回転方向ゆらぎ / 一分子観察 / 張力 / 脱重合 / 蛍光ラベル / 機械受容 / 量子ドット / 近接場光 |
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| Research Abstract |
When forces were applied to the filament, it was severed after the application of cofilin with a significantly larger delay in comparison with the control filaments suspended in solution. The binding rate of cofilin to an actin bundle was decreased when the bundle was tensed. These results demonstrate that tension in the actin filament prevents the binding of cofilin, resulting in a decrease in the effective severing activity of cofilin.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Roles of disrupted-in-schizophrenia 1-interacting protein girdin in postnatal development of the dentate gyrus.2009
Author(s)
Enomoto A, Asai N, Namba T, Wang Y, Kato T, Tanaka M, Tatsumi H, Taya S, Tsuboi D, Kuroda K, Kaneko N, Sawamoto K, Miyamoto R, Jijiwa M, Murakumo Y, Sokabe M, Seki T, Kaibuchi K, Takahashi M.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Roles of disrupted-in-schizophrenia 1-interacting protein girdin in postnatal development of the dentate gyrus2009
Author(s)
Enomoto A, Asai N, Namba T, Wang Y, Kato.T, Tanaka M, Tatsumi H, Taya S, Tsuboi D, Kuroda K, Kaneko N, Sawamoto K, Miyamoto R, Jijiwa M, Murakumo Y, Sokabe M, Seki T, Kaibuchi K, Takahashi M.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article]2009
Author(s)
辰巳仁史
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Journal Title
第3巻「動物の「動き」の秘密にせまる」 : 接着構造と細胞骨格を用いた細胞移動(共立出版)
Pages: 130-152
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