| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
P2X4 receptor, a non-selective ATP-operated cation channel, in vascular endothelial cells (ECs) mediates flow-induced Ca^<2+> influx and subsequent NO production to regulate vascular tone. Although P2X4 is thought to be activated by the ATP released into the extracellular space from ECs by shear stress, our fluorescence microscopic observation in ECs without shear stress showed that majority of P2X4 molecules is localized on the intracellular vesicles. In this study we found that the expression level of cell surface P2X4 of ECs is up-regulated by shear stress, leading to an apparent increase in the sensitivity to external ATP.
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