| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Research Abstract |
The folding mechanisms of two globular proteins, staphylococcal nuclease and horse apomyoglobin, were investigated by using the ultrarapid mixing technique combined with the mutation analysis. Staphylococcal nuclease molecules were found to be already compact within the β-barrel domain several millisecond after the initiation of the folding reaction. Apomyoglobin accumulated at least one intermediate 100 microseconds after the initiation of the folding reaction, followed by the accumulation of at least two intermediate in the sequential pathway, before reaching the native state.
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