Development of observation technology with electron microscope of force-generating myosin
| Project/Area Number |
20570155
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
YASUNAGA Takuo Kyushu Institute of Technology, 大学院・情報工学研究院, 教授 (60251394)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 運動・輸送 / ミオシン / 筋肉 / アクチン / 電子顕微鏡 / 力発生 / クライオ電子顕微鏡法 / 急速凍結装置 / トモグラフィー / 分子モーター / 画像処理 |
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| Research Abstract |
We tried to develop observation technology with electron microscopes of force generating myosin. One necessary technology is to observe the active interaction between a myosin filament and an actin filament during force generation. The actin filament could be, in success, pulled by a magnetic bead, which was linked to the actin covalently. The other is to observe three-dimensional structure of single myosin head and actin filament by electron cryo-microscopy. We developed new three-dimensional reconstruction programs for single particle analysis and electron tomography of frozen-hydrated specimens and obtained highly-resolved 3D map of actin filaments/actomyosin complexes and preliminary 3D maps of single head myosin bound to a single actin filament.
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Report
(4 results)
Research Products
(68 results)
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[Journal Article] Structural basis for actin assembly, activation of ATP hydrolysis, and delayed phosphate release2010
Author(s)
Murakami, K., Yasunaga, T., Noguchi, T.Q.P., Gomibuchi, Y., Ngo, K.X., Uyeda, T.Q.P., Wakabayashi, T.
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Journal Title
Cell 143:2
Pages: 275-287
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Peer Reviewed
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[Presentation] Eosプラットフォーム:GPGPUならびに立体視テクノロジー2009
Author(s)
我妻竜三, 金明月, 高崎寛子, 富永彰人,福地弘晃, 大田興希, 白川圭太, 塚田祥弘, 鶴崎聖也, 吉竹純一, 安永卓生
Organizer
日本顕微鏡学会九州支部会
Place of Presentation
九州工業大学,北九州市
Year and Date
2009-12-06
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[Presentation] Development of Eos : efficient 3D reconstruction of biomolecules from electron micrographs by enabling GPU computing and use of stereoscopic devices2009
Author(s)
Azuma R., Jim M., Takazaki H., Tominaga A., Fukuchi H., Ohta K., Shirakawa K., Tsukada Y., Tsurusaki S., Yoshitake J., Yasunaga T.,
Organizer
第47回生物物理学会年会
Place of Presentation
アクティとくしま・徳島文理大学徳島キャンパス,徳島
Related Report
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[Presentation] Development of Eos : efficient 3D reconstruction of biomolecules from electron micrographs by enabling GPU computing and use of stere oscopic devices2009
Author(s)
Ryuzo Azuma, Mingyue Jim, Hiroko Takazaki, Akito Tominaga, Hiroaki Fukuchi, Koki Ohta, Keita Shirakawa, Yoshihiro Tsukada, Seiya Tsurusaki, Junnichi Yoshitake, Takuo Yasunaga
Organizer
第47回生物物理学会年会
Place of Presentation
アクティとくしま・徳島文理大学徳島キャンパス, 徳島
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[Presentation] Development of Electron Tomography Systems integrated with our Developed Image Analysis Environment, Eos2008
Author(s)
Yasunaga T., Goto T., Imamura M., Matuura A., Murayama R., Niina H., Takimoto Y., Iwasaki K., Miyazawa A.
Organizer
9th Asia-Pacific Microscopy Conference
Place of Presentation
Jeju, Korea
Related Report
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