Development of postgenomic gene manipulation system for the extreme thermophile Thermus thermophilus
| Project/Area Number |
20570156
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
TAMAKOSHI Masatada Tokyo University of Pharmacy and Life Science, 生命科学部, 准教授 (10277254)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 高度好熱菌 / ベクター / ファージ / 膜蛋白質 / 挿入配列 / 線毛 / 染色体工学 / インテグレーションベクター / PilQ / ヒスチジンタグ / FLAGタグ / ATP合成酵素 / PilD |
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| Research Abstract |
His-tagged and FLAG-tagged membrane protein complexes were expressed in the extreme thermophile Thermus thermophilus by transforming with improved integration vectors, which enable us to detect and prepare the membrane protein complexes efficiently. Furthermore, in order to develop a phage vector for the thermophile, the gene for the receptor on the thermophile was identified by constructing a gene-knockout strain. Finally, a functional insertion sequence in the thermophile, which is considered to be a tool to construct the thermophile's mutant library, was found in a leu axotrophic mutant strain and the nucleotide sequence was analyzed.
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Report
(4 results)
Research Products
(42 results)
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[Journal Article]
Author(s)
Jormakka M., Yokoyama K., Yano T., Tamakoshi M., Akimoto S., Shimamura T., Curmi P., Iwata S.
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Journal Title
Nat.Struct.Mol.Biol. 15
Pages: 730-737
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