| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
His-tagged and FLAG-tagged membrane protein complexes were expressed in the extreme thermophile Thermus thermophilus by transforming with improved integration vectors, which enable us to detect and prepare the membrane protein complexes efficiently. Furthermore, in order to develop a phage vector for the thermophile, the gene for the receptor on the thermophile was identified by constructing a gene-knockout strain. Finally, a functional insertion sequence in the thermophile, which is considered to be a tool to construct the thermophile's mutant library, was found in a leu axotrophic mutant strain and the nucleotide sequence was analyzed.
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