Analysis of yeast Cch1, a homologue of animal voltage-gated calcium channel pore subunit, and its regulatory factor Mid1.

• Project/Area Number: 20570158
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Tokyo Metropolitan Organization for Medical Research
• Principal Investigator: IIDA Kazuko Tokyo Metropolitan Organization for Medical Research, 東京都臨床医学総合研究所, 主任研究員 (40151229)
• Co-Investigator(Kenkyū-buntansha): IIDA Hidetoshi 東京学芸大学, 教育学部, 教授 (70124435)
• Research Collaborator: TENG Jinfeng 群馬大学, 医学系研究科, 博士課程院生
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 生体膜 / 受容体 / カルシウムチャネル / チャンネル

Research Abstract

Cch1 is a yeast homologue of the pore-forming α1 subunit of animal voltage-gated calcium channels (VGCC), and Mid1 is an essential component together with Cch1 for the high-affinity calcium uptake in yeast. We tried to detect electric currents through the Cch1/Mid1 channel expressed in cultured mammalian cells or in yeast spheroplasts by the patch-clump method. Mid1 is heavily N-glycosylated allover the length, and Mid1 has a cysteine-rich motif in the C-terminal region. These characteristics suggest that Mid1 is a functional homologue of the α_2/δsubunit of animal VGCCs.

Research Products

• [Journal Article] Role of glycine residues highly conserved in the S2-S3 linkers of domain I and II of voltage-gated calcium channel α1 subunits. Biochem. (2010)
  • Author(s): Teng J., Iida K., Ito M., Izumi-Nakaseko H., Kojima I., Adachi-Akahane S., Iida H.
  • Journal Title: Biophys.Acta-Biomembranes 1798 Pages: 966-974
  • Peer Reviewed
• [Journal Article] MCA1 and MCA2 that mediate Ca^<2+> uptake have distinct and overlapping roles in Arabitopsis thaliana. (2010)
  • Author(s): Yamanaka T., Nakagawa Y., Mori K., Nakano M., Imamura T., Kataoka H., Terashima A., Iida K., Kojima I., Katagiri T., Shinozaki K., Iida H.
  • Journal Title: Plant Physiol. 152 Pages: 1284-1296
  • Peer Reviewed
• [Journal Article] MCA1 and MCA2 that mediate Ca^<2+> uptake have distinct and overlapping roles in Arabitopsis thaliana. (2010)
  • Author(s): Yamanaka, T., Nakagawa, Y., Mori, K., Nakano, M., Imamura, T., Kataoka, H., Terashima, A., Iida, K., Kojima, I., Katagiri, T., Shinozaki, K., Iida, H.
  • Journal Title: Plant Physiol. Volume: 152 Pages: 1284-1296
  • Peer Reviewed
• [Journal Article] Role of glycine residues highly conserved in the S2-S3 linkers of domain I and II of voltage-gated calcium channel ai subunits (2009)
  • Author(s): Teng, J., Iida, K., Ito, M., Izumi-Nakaseko, H., Kojima, I., Adachi-Akahane, S., Iida, H.
  • Journal Title: Biochem.Biophys.Acta 1798 Pages: 966-974
  • Peer Reviewed
• [Journal Article] Ion-channel blocker sensitivity of voltage-gated calcium-channel homologue Cch1 in Saccharomyces cerevisuae. (2008)
  • Author(s): Teng J., Goto R., Iida K., Kojima I., Iida H.
  • Journal Title: Microbiology 154 Pages: 3775-3781
  • Peer Reviewed
• [Journal Article] Ion-channel blocker sensitivity of voltage-gated calcium-channel homologue Cchl in Saccharomyces cerevisuae (2008)
  • Author(s): Teng, J., Goto, R., Iida, K., Kojima, I., Iida, H.
  • Journal Title: Microbiology 154 Pages: 3775-3781
• [Presentation] カルシウム透過性機械受容チャネルの構造と機能 (2010)
  • Author(s): 飯田秀利、中野正貴、原茂恵美子、今村朋美、飯田和子、重松秀樹、永山國昭
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] シロイヌナズナのCa^<2+>透過性機械受容チャネル候補の酵母発現系を用いた構造と機能の解析 (2010)
  • Author(s): 中野正貴、飯田和子、丹生谷博、飯田秀利
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Molecular study on Arabitopsis Ca^<2+> permeable mechanosensitive channel candidates using yeast. (2010)
  • Author(s): Nakano, M., Iida, K., Nyunoya, H, Iida, H.
  • Organizer: 第21回シロイヌナズナ国際会議
  • Place of Presentation: 横浜
  • Year and Date: 2010-06-07
• [Presentation] Role of glycine residues highly conserved in the domain I/II S2-S3 linkers of voltage-gated calcium channel α1 subunits. (2009)
  • Author(s): Teng J., Izumi-Nakaseko H., Iida K., Ito M., Kojima I., Adachi-Akahane S., Iida H.
  • Organizer: XXXVIth International Congress of Physiological Sciences
  • Place of Presentation: 京都
  • Year and Date: 2009-07-27
• [Presentation] Role of glycine residues highly conserved in the domain I/II S2-S3 linkers of voltage-gated calcium channel α1 subunits (2009)
  • Author(s): Teng, J., Izumi-Nakaseko, H., Iida, K., Ito, M., Kojima, I., Adachi-Akahane, S., Iida, H.
  • Organizer: XXXVIth International Congress of Phvsiological Sciences
  • Place of Presentation: 京都府・国立京都国際会議センター
• [Presentation] 電位作動性カルシウムチャネルα1サブユニットS2-S3リンカーに保存されているGly残基の役割 (2008)
  • Author(s): 滕金風、中瀬古(泉)寛子、飯田和子、小島至、赤羽悟美、飯田秀利
  • Organizer: 第31回日本分子生物学会・第81回日本生化学会合同年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 電位作動性カルシウムチャネルα1サブユニットS2-S3リンカーに保存されているGly残基の役割 (2008)
  • Author(s): 縢金風, 中瀬古(泉)寛子, 飯田和子, 小島至, 赤羽悟美, 飯田秀利
  • Organizer: 日本分子生物学会・日本生化学会合同年会
  • Place of Presentation: 神戸