Project/Area Number |
20570158
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biophysics
|
Research Institution | Tokyo Metropolitan Organization for Medical Research |
Principal Investigator |
IIDA Kazuko Tokyo Metropolitan Organization for Medical Research, 東京都臨床医学総合研究所, 主任研究員 (40151229)
|
Co-Investigator(Kenkyū-buntansha) |
IIDA Hidetoshi 東京学芸大学, 教育学部, 教授 (70124435)
|
Research Collaborator |
TENG Jinfeng 群馬大学, 医学系研究科, 博士課程院生
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 生体膜 / 受容体 / カルシウムチャネル / チャンネル |
Research Abstract |
Cch1 is a yeast homologue of the pore-forming α1 subunit of animal voltage-gated calcium channels (VGCC), and Mid1 is an essential component together with Cch1 for the high-affinity calcium uptake in yeast. We tried to detect electric currents through the Cch1/Mid1 channel expressed in cultured mammalian cells or in yeast spheroplasts by the patch-clump method. Mid1 is heavily N-glycosylated allover the length, and Mid1 has a cysteine-rich motif in the C-terminal region. These characteristics suggest that Mid1 is a functional homologue of the α_2/δsubunit of animal VGCCs.
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