| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
Depletion of Mcm10 by siRNA inhibited origin firing, not fork progression. Using truncated mutants, I found Mcm10 interacted with Mcm2-7 through the intrinsically disorder domain. In addition, the genetical analyses showed that the conserved domain was essential for DNA replication. On the contrary, the Mcm2-7-interacting domain contributed to the retention of Mcm10 onto chromatin.
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