Mechanisms of genomic stability maintenance by replication scaffold protein, AND-1

• Project/Area Number: 20570173
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Tokyo Metropolitan Organization for Medical Research
• Principal Investigator: YOSHIZAWA Naoko Tokyo Metropolitan Organization for Medical Research, 東京都臨床医学総合研究所, 主任研究員 (30344071)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: DNA複製 / 複製チェックポイント / 複製フォーク / 複製ストレス / DNA修復 / ゲノム不安定性 / 姉妹染色体交換 / AND-1 / Timeless / 修復組換え / ゲノム安定性 / 染色体接着 / コヒーシン / 相同組換え修復 / DNA損傷

Research Abstract

Coordinated execution of DNA replication, checkpoint activation and postreplicative chromatid cohesion is intimately related to the replication fork machinery. Human AND-1 is localized at the replication scaffold, and is required for efficient DNA synthesis and its depletion increases DNA damage, delays progression of S phase, leads to cell death in cancer cells. AND-1 is also required for checkpoint activation in replication stress, inhibition of UV-RDS and homologous recombinational repair. This study indicates that AND-1 coordinates multiple cellular events in S phase and G2 phase.

Research Products

• [Journal Article] Eukaryotic DNA Replication : where, when and how? (2010)
  • Author(s): Hisao Masai, Seiji Matsumoto, Zhiying You, Naoko Yoshizawa-Sugata, Masako Oda
  • Journal Title: Annual Review of Biochemistry 79 Pages: 89-130
  • Peer Reviewed
• [Journal Article] Eukaryotic chromosome DNA replication : where, when, and how? (2010)
  • Author(s): Masai H, Matsumoto S, You Z, Yoshizawa-Sugata N, Oda M
  • Journal Title: Annu.Rev.Biochem. Volume: 79 Pages: 89-130
  • Peer Reviewed
• [Journal Article] Eukaryotic DNA Replication : where, when and how? (2010)
  • Author(s): Masai, H., Matsumoto, S., You, Z., Yoshizawa-Sugata, N., Oda, M.
  • Journal Title: Ann.Rev.Biochem. (印刷中)
  • Peer Reviewed
• [Journal Article] Roles of human AND-1 in chromosome transactions in S phase. (2009)
  • Author(s): Naoko Yoshizawa-Sugata, Hisao Masai
  • Journal Title: J. Biol. Chem. 284(31) Pages: 20718-20728
  • Peer Reviewed
• [Journal Article] Roles of human AND-1 in chromosome transactions in S phase (2009)
  • Author(s): Yoshizawa-Sugata N., Masai H.
  • Journal Title: J.Biol.Chem. Vol.284 Pages: 20718-20728
  • Peer Reviewed
• [Presentation] Regulation of replication program and maintenance of genetic integrity (2010)
  • Author(s): Hisao Masai, Satoshi Yamazaki, Motoshi Hayano, Yutaka Kanoh, Seiji Matsumoto, Naoko Kakusho, Ai, Ishii, Michie Shimmoto, Masako Oda, Naoko Yoshizawa, Sayuri Itoh, Satomi Kudo
  • Organizer: 第33回日本分子生物学会(BMB2010)
  • Place of Presentation: 神戸ポートアイランド(口頭発表)
  • Year and Date: 2010-12-10
• [Presentation] Regulation of replication program and maintenance of genetic integrity (2010)
  • Author(s): Hisao Masai, Satoshi Yamazaki, Motoshi Hayano, Yutaka Kanoh, Seiji Matsumoto, Naoko Kakusho, Ai, Ishii, Michie Shimmoto, Masako Oda, Naoko Yoshizawa, Sayuri Itoh, Satomi Kudo
  • Organizer: 第33回日本分子生物学会(BMB2010)
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] Regulation of origin firing program and checkpoint by Cdc7, Mrc1 and Rif1 (2010)
  • Author(s): Hisao Masai, Seiji Matsumoto, Motoshi Hayano, Yutaka Kanoh, Satoshi Yamazaki, Satomi Kudo, Naoko Kakusho, Rino Fukatsu, Naoko Yoshizawa, Ai, Ishii
  • Organizer: 7th 3R Symposium
  • Place of Presentation: Toyama conference center, Toyama(口頭発表)
  • Year and Date: 2010-10-29
• [Presentation] Regulation of origin firing program and checkpoint by Cdc7, Mrc1 and Rif1 (2010)
  • Author(s): Hisao Masai, Seiji Matsumoto, Motoshi Hayano, Yutaka Kanoh, Satoshi Yamazaki, Satomi Kudo, Naoko Kakusho, Rino Fukatsu, Naoko Yoshizawa, Ai, Ishii
  • Organizer: 7th 3R(Replication, Recombination & Repair)meeting 2010
  • Place of Presentation: Toyama conference center(Toyama)
  • Year and Date: 2010-10-29
• [Presentation] Roles of human replisome component AND-1 and Tim in S phase chromosome transaction (2010)
  • Author(s): Naoko Yoshizawa-Sugata, Hisao Masai
  • Organizer: 7th 3R Symposium (ポスター発表)
  • Place of Presentation: Toyama conference center, Toyama
  • Year and Date: 2010-10-28
• [Presentation] Roles of human replisome component AND-1 and Tim in S phase chro-mosome transaction (2010)
  • Author(s): Naoko Yoshizawa, Hisao Masai
  • Organizer: 7th 3R(Replication, Recombination & Repair)meeting 2010
  • Place of Presentation: Toyama conference center(Toyama)
  • Year and Date: 2010-10-28
• [Presentation] Human AND-1 is required for efficient DNA replication, checkpoint activation and homologous recombinational repair (2009)
  • Author(s): Yoshizawa-Sugata N., Masai H.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜パシフィコ(神奈川県)(英語での口頭発表)
  • Year and Date: 2009-12-09
• [Presentation] Human AND-1 is required for efficient DNA replication, checkpoint activation and homologous recombinational repair (2009)
  • Author(s): Naoko Yoshizawa, Hisao Masai
  • Organizer: 第32回日本分子生物学会年会(口頭・ポスター発表)
  • Place of Presentation: パシフィコ横浜
• [Presentation] Human AND-1 is required for efficient DNA replication, checkpoint activation and homologous recombinational repair (2008)
  • Author(s): Naoko Yoshizawa-Sugata, Hisao Masai
  • Organizer: 6th 3R Symposium (ポスター発表)
  • Place of Presentation: Tsumagoi, Shizuoka
• [Presentation] Regulation of replication fork in maintenance of genomic integrity (2008)
  • Author(s): Hisao Masai, Motoshi Hayano, Seiji Matsumoto, Yutaka Kanoh, Michie Shimmoto, Mika Yokoyama, Taku Tanaka, Naoko Kakusho, Naoko Yoshizawa, You Zhiying, Sayuri Ito, Chika Taniyama, Ai Ishii
  • Organizer: 6th 3R Symposium
  • Place of Presentation: Tsumagoi, Shizuoka(招待講演)
• [Presentation] Human AND-1 is required for efficient DNA replication, checkpoint activation and homologous recombination repair (2008)
  • Author(s): Naoko Yoshizawa-Sugata, Hisao Masai
  • Organizer: EMBO Conference on Replication and Segregation of Chromosomes (ポスター発表)
  • Place of Presentation: Geilo, Norway
• [Presentation] Regulation of replication fork integrity during S phase (2008)
  • Author(s): Hisao Masai, Motoshi Hayano, Seiji Matsumoto, Yutaka Kanoh, Mika Yokoyama, Michiyo Shimmoto, Naoko Kakusho, Naoko Yoshizawa, Taku Tanaka
  • Organizer: 第31回日本分子生物学会(BMB2008)(シンポジウム口頭発表)
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Regulation of establishment and maintenance of replication fork by Cdc7 kinase (2008)
  • Author(s): Hisao Masai, Motoshi Hayano, Seiji Matsumoto, Naoko Kakusho, Mika Yokoyama, Yutaka Kanoh, Michie Simmoto, Taku Tanaka
  • Organizer: EMBO Conference on "Replication and Segregation of Chromosomes"
  • Place of Presentation: Geilo, Norway
• [Presentation] Human AND-1 is required for efficient DNA replication, checkpoint activation and homologous recombination repair (2008)
  • Author(s): Naoko Yoshizawa-Sugata, Hisao Masai
  • Organizer: EMBO Conference on "Replication and Segregation of Chromosomes"
  • Place of Presentation: Geilo, Norway
• [Presentation] Human AND-1 is required for efficient DNA replication, checkpoint activation and homologous recombinational repair (2008)
  • Author(s): Naoko Yoshizawa-Sugata, Hisao Masai
  • Organizer: 6th 3R (Replication, Repair, Recombination) Symposium
  • Place of Presentation: Tsumagoi, Shizuoka
• [Presentation] Regulation of replication fork in maintenance of genomic integrity (2008)
  • Author(s): Hisao Masai, Motoshi Hayano, Seiji Matsumoto, Yutaka Kanoh, Michie Shimmoto, Mika Yokoyama, Taku Tanaka, Naoko Kakusho, Noako Yoshizawa, You Zhiying, Sayuri Ito, Chika Taniyama, Ai Ishii
  • Organizer: 6th 3R (Replication, Repair, Recombination) Symposium
  • Place of Presentation: Tsumagoi, Shizuoka
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