Global identification and functional analysis of protein kinase substrates by a phosphoproteomic approach

• Project/Area Number: 20570177
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: The University of Tokushima
• Principal Investigator: KOSAKO Hidetaka The University of Tokushima, 疾患酵素学研究センター, 准教授 (10291171)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: プロテオーム / タンパク質キナーゼ / リン酸化 / MAPキナーゼ / 核膜孔複合体 / 核-細胞質間輸送 / 抗リン酸化抗体 / プロテインキナーゼ / プロテアソーム / PHドメイン / siRNA / セミインタクト細胞 / プロテオミクス / 2D-DIGE / IMAC

Research Abstract

A multi-step method for detecting proteins phosphorylated by ERK MAP kinase reveals many new ERK substrates, including three nucleoporins. Nup50 is phosphorylated in FG repeats by ERK in vitro and in vivo, suggesting a new mechanism by which MAP kinase signaling controls nuclear translocation of proteins.

Research Products

• [Journal Article] Quantitative phosphoproteomics strategies for understanding protein kinase-mediated signal transduction pathways. (2011)
  • Author(s): Kosako, H., Nagano, K.
  • Journal Title: Expert Rev. Proteomics 8 Pages: 81-94
  • Peer Reviewed
• [Journal Article] Quantitative phosphoproteomics strategies for understanding protein kinase-mediated signal transduction pathways (2011)
  • Author(s): H.Kosako, et al.
  • Journal Title: Expert Review of Proteomics Volume: 8 Pages: 81-94
  • Peer Reviewed
• [Journal Article] Phosphorylation of nucleoporins : signal transduction-mediated regulation of their interaction with nuclear transport receptors. (2010)
  • Author(s): Kosako, H., Imamoto, N.
  • Journal Title: Nucleus 1 Pages: 309-313
  • Peer Reviewed
• [Journal Article] リン酸化プロテオミクスで明らかとなったERKによる核膜孔複合体の機能制御 (2010)
  • Author(s): 小迫英尊
  • Journal Title: 実験医学 28 Pages: 69-73
• [Journal Article] Phosphorylation of nucleoporins : signal transduction-mediated regulation of their interaction with nuclear transport receptors (2010)
  • Author(s): H.Kosako, et al.
  • Journal Title: Nucleus Volume: 1 Pages: 309-313
  • Peer Reviewed
• [Journal Article] Phosphoproteomics reveals new ERK MAP kinase targets and links ERK to nucleoporin-mediated nuclear transport. (2009)
  • Author(s): Kosako, H., Yamaguchi, N., Aranami, C., Ushiyama, M., Kose, S., Imamoto, N., Taniguchi, H., Nishida, E., Hattori, S.
  • Journal Title: Nature Struct.Mol.Biol. 16 Pages: 1026-1035
  • Peer Reviewed
• [Journal Article] (2009)Phos-tag Western blotting for detecting stoichiometric protein phosphorylation in cells. (2009)
  • Author(s): Kosako, H.
  • Journal Title: Nature Protoc.(Protocol Exchange) 170
• [Journal Article] Phosphorylation of Nephrin triggers Ca^<2+> signaling by recruitment and activation of phospholipase C-γ1. (2009)
  • Author(s): Harita, Y., Kurihara, H., Kosako, H., Tezuka, T., Sekine, T., Igarashi, T., Ohsawa, I., Ohta, S., Hattori, S.
  • Journal Title: J.Biol.Chem. 284 Pages: 8951-8962
  • Peer Reviewed
• [Journal Article] Phosphoproteomics reveals new ERK MAP kinase targets and links ERK to nucleoporin-mediated nuclear transport. (2009)
  • Author(s): H.Kosako, et al.
  • Journal Title: Nature Struct.Mol.Biol. 16 Pages: 1026-1035
  • Peer Reviewed
• [Journal Article] Phos-tag Western blotting for detecting stoichiometric protein Phosphorylation in cells. (2009)
  • Author(s): H.Kosako
  • Journal Title: Nature Protocols
• [Journal Article] Phosphorylation of Nephrin triggers Ca^<2+> signaling by recruitment and activation of phospholipase C-γ1. (2009)
  • Author(s): Y. Harita, et al.
  • Journal Title: J. Biol. Chem. 284 Pages: 8951-8962
  • Peer Reviewed
• [Journal Article] Identification of protein kinase substrates by proteomic approaches. (2008)
  • Author(s): Hattori, S., Iida, N., Kosako, H.
  • Journal Title: Expert Rev.Proteomics 5 Pages: 497-505
  • Peer Reviewed
• [Journal Article] Neph1, acomponent of the kidney slit diaphragm,is tyrosine phosphorylated by the Src family tyrosine kinase and modulates intracellular signaling by binding to Grb2. (2008)
  • Author(s): Harita, Y., Kurihara, H., Kosako, H., Tezuka, T., Sekine, T., Igarashi, T., Hattori, S.
  • Journal Title: J.Biol.Chem. 283 Pages: 9177-9186
  • Peer Reviewed
• [Journal Article] Neph1, a component of the kidney slit diaphragm, is tyrosine-phosphorylated by the Src family tyrosine kinase and modulates intracellular signaling by binding to Grb2. (2008)
  • Author(s): Y. Harita, et al.
  • Journal Title: J. Biol. Chem. 283 Pages: 9177-9186
  • Peer Reviewed
• [Journal Article] Identification of protein kinase substrates by proteomic approaches. (2008)
  • Author(s): S. Hattori, et al.
  • Journal Title: Expert Rev. Proteomics 5 Pages: 497-505
  • Peer Reviewed
• [Presentation] ERK/MAPキナーゼなどによるFGヌクレオポリンのリン酸化は核輸送因子との相互作用を制御する (2010)
  • Author(s): 小迫英尊, ら
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Phosphoproteomics reveals ERK MAP kinase-mediated regulation of nuclear pore complex proteins. (2010)
  • Author(s): Kosako, H.
  • Organizer: BIT's 3^<rd> World Cancer Congress-2010
  • Place of Presentation: シンガポール
  • Year and Date: 2010-06-23
• [Presentation] Phosphorylation of nuclear pore complex proteins by ERK MAP kinase regulates interaction with transport receptors. (2009)
  • Author(s): Kosako, H., et al.
  • Organizer: ASCB2009
  • Place of Presentation: San Diego, USA
  • Year and Date: 2009-12-06
• [Presentation] Phosphorylation of nuclear pore complex proteins by ERK MAP kinase regulates interaction with transport receptors (2009)
  • Author(s): H.Kosako, et al.
  • Organizer: ASCB2009
  • Place of Presentation: San Diego, USA
  • Year and Date: 2009-12-06
• [Presentation] リン酸化プロテオミクスによって明らかとなったERK/MAPキナーゼによる核膜孔複合体の機能制御 (2009)
  • Author(s): 小迫英尊, ら
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-24
• [Presentation] リン酸化プロテオミクスによって明らかとなったERK/MAPキナーゼによる核膜孔複合体の機能制御 (2009)
  • Author(s): 小迫英尊, 他
  • Organizer: 第82回 日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] Phosphoproteomic profiling of ERK MAP kinase signaling reveals a role of phosphorylation in the interaction of nucleoporins with transport factors. (2008)
  • Author(s): Kosako, H., et al.
  • Organizer: ASCB2008
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2008-12-15
• [Book] タンパク質の事典(分担執筆、猪飼篤ら編) (2008)
  • Author(s): 小迫英尊、後藤由季子
  • Total Pages: 863
  • Publisher: 朝倉書店
• [Book] タンパク質の事典(分担執筆、猪飼篤ら, 編) (2008)
  • Author(s): 小迫 英尊, 他
  • Total Pages: 863
  • Publisher: 朝倉書店
• [Remarks] ホームページ等
  • URL: http://pub2.db.tokushima-u.ac.jp/ERD/person/172252/profile-ja.html
• [Remarks]
  • URL: http://pub2.db.tokushima-u.ac.jp/ERD/person/172252/profile-ja.html