The identification of signal components and analysis of the regulatory mechanisms of WNK protein kinases, the essential genes for early development and pathogenesis of hereditary form of hypertension

• Project/Area Number: 20570179
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East
• Principal Investigator: MORIGUCHI Tetsuo National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East, プロテオ医学研究センター, 助教 (40323571)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: WNK / シグナル伝達 / 疾患 / 発生 / がん / 初期発生

Research Abstract

WNK kinase family that has been recently identified serine-threonine protein kinase family conserved among several multicellular organisms. There are four human WNK family members, and mutations in two of them, WNK1 and WNK4, have been linked to a hereditary form of human hypertension. Moreover, the disruption of the WNK1 gene caused death of organisms. Therefore, the elucidation of WNK signaling network is important upon clarifying the mechanism of morphogenesis, organogenesis and disease. In this research, new interacting factors of WNK were identified by biochemical, molecular biology, and genetic techniques.

Research Products

• [Journal Article] Involvement of LKB1 in Epithelial-Mesenchymal Transition (EMT) of Human Lung Cancer Cells (2011)
  • Author(s): Roy BC, Kohno T, Iwakawa R, Moriguchi T, Kiyono T, Morishita K, Sanchez-Cespedes M, Akiyama T, Yokota J
  • Journal Title: Lung Cancer vol. 70 Pages: 136-145
  • Peer Reviewed
• [Journal Article] Involvement of LKB1 in epithelial-mesenchymal transition (EMT) of human lung cancer cells (2010)
  • Author(s): Roy, BC
  • Journal Title: Lung Cancer Volume: 70 Pages: 136-145
  • Peer Reviewed
• [Journal Article] Involvement of LKB1 in epithelial-mesenchymal transition(EMT)of human lung cancer cells (2010)
  • Author(s): Roy, BC
  • Journal Title: Lung Cancer (in press)
  • Peer Reviewed
• [Presentation] KRAS/LKB1変異による肺腺がん細胞のEMTとがん幹細胞マーカーの発現誘導 (2009)
  • Author(s): 森口徹生
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] KRAS/LKB1変異による肺線がん細胞のEMTとがん幹細胞マーカーの発現誘導 (2009)
  • Author(s): 森口徹生
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] Involvement of LKB1 in epithelial-mesenchymal transition (EMT) of human lung cancer cells (2009)
  • Author(s): 森口徹生
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-03
• [Presentation] Involvement of LKB1 in Epithelial-Mesenchymal Transition(EMT)in Human Lung Cancer (2009)
  • Author(s): 森口徹生
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-03
• [Presentation] LKB1 inactivation triggers EMT through the induction of transcriptional repressor ZEB1 in human lung cancer (2008)
  • Author(s): 森口徹生, et al
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] LKB1 inactivation triggers EMT through the induction of transcriptional repressor ZEB1 in human lung cancer (2008)
  • Author(s): Roy, BC, 河野隆志, 岩川麗香, 森口徹生, 他4名
  • Organizer: BMB2008(第31回日本分子生物学会年会第81回日本生化学会大会合同大会)
  • Place of Presentation: 神戸
• [Remarks] ホームページ等
  • URL: http://www.tmd.ac.jp/mri/mri-mcb/index_j.html
• [Remarks]
  • URL: http://www.proteo.ehime-u.ac.jp/section/07.html
• [Remarks]
  • URL: http://www.tmd.ac.jp/mri/mri-mcb/index_j.html