| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
The JNK MAP kinase (MAPK) pathway plays a pivotal role in the various stress responses of evolutionarily diverse species. In C. elegans, a JNK-like MAPK pathway composed of MLK-1 MAPKKK, MEK-1 MAPKK and KGB-1 MAPK is known to act in a heavy metal stress response. However, the upstream or downstream components of this pathway remain unknown. It has been shown that the KGB-1 pathway is negatively regulated by VHP-1, a dual-specificity MAPK phosphatase, and that mutations defective in the KGB-1 pathway suppress the growth arrest caused by a loss-of-function mutation in the vhp-1 gene. Therefore, we performed genome-wide RNAi screening for vhp-1 suppressor to identify genes functioning in the KGB-1 pathway. As the result, we identified several genes including novel genes as vhp-1 suppressors. In addition, we identified an adaptor protein SHC-1, a protein kinase MAX-2 and a small G protein MIG-2 as upstream factors of MLK-1 and clarified their roles in KGB-1 cascade.
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