Analysis of the potential roles of Nucleoporin in oncogenesis

• Project/Area Number: 20570184
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: OKA Masahiro Osaka University, 大学院・生命機能研究科, 助教 (40432504)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ヌクレオポリン / 核外輸送 / Nup98 / Crm1 / がん化 / Crml

Research Abstract

Nup98 is a frequent target of chromosomal rearrangements in leukemia, which is associated with the disease development. Our study revealed that Nup98 binds to Crm1, a nuclear export factor, and is involved in protein export from the nucleus to the cytoplasm. In addition, we demonstrated that the expression of Nup98-HoxA9 fusion inhibits the nuclear export.

Research Products

• [Journal Article] Nup358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesis. (2011)
  • Author(s): Asally, M
  • Journal Title: FEBS J Volume: 278 Pages: 610-621
  • Peer Reviewed
• [Journal Article] Nup358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesis. (2010)
  • Author(s): Asally M, Yasuda Y, Oka M, Otsuka S, Yoshimura SH, Takeyasu K, Yoneda Y.
  • Journal Title: FEBS J. 278 Pages: 610-621
  • Peer Reviewed
• [Journal Article] The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export. (2010)
  • Author(s): Oka M, Asally M, Yasuda Y, Ogawa Y, Tachibana T, Yoneda Y.
  • Journal Title: Mol Biol Cell. 21 Pages: 1885-1896
  • Peer Reviewed
• [Journal Article] Two isoforms of npap60 (nup50) differentially regulate nuclear protein import. (2010)
  • Author(s): Ogawa Y, Miyamoto Y, Asally M, Oka M, Yasuda Y, Yoneda Y.
  • Journal Title: Mol Biol Cell. 21 Pages: 630-638
  • Peer Reviewed
• [Journal Article] The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export. (2010)
  • Author(s): Oka, M
  • Journal Title: Mol Biol Cell Volume: 21 Pages: 1855-1896
  • Peer Reviewed
• [Journal Article] Effective culture conditions for the induction of pluripotent stem cells. (2010)
  • Author(s): Okada, M
  • Journal Title: Biochem Biophys Acta Volume: 1800 Pages: 956-953
  • Peer Reviewed
• [Journal Article] Specific monoclonal antibody against the nuclear pore complex protein, Nup96. (2010)
  • Author(s): Mizuguchi, C
  • Journal Title: Hybridoma Volume: 29 Pages: 551-553
  • Peer Reviewed
• [Journal Article] Two isoforms of Npap60 (Nup50) differentially regulate nuclear protein import (2010)
  • Author(s): Ogawa, Y
  • Journal Title: Mol.Biol.Cell 21 Pages: 630-638
  • Peer Reviewed
• [Presentation] Nucleo-cytoplasmic shuttling of Oct-4 (2010)
  • Author(s): Oka, M
  • Organizer: BMB 2010
  • Place of Presentation: Kobe
  • Year and Date: 2010-12-08
• [Presentation] Targeted disruption of one of the importin-α5 family member sleads to female functional incompetence in delivery (2010)
  • Author(s): Moriyama, T
  • Organizer: BMB 2010
  • Place of Presentation: Kobe
  • Year and Date: 2010-12-08
• [Presentation] Targeted disruption of the nuclear import adaptor, importin-α5, leads to female genital hypoplasia (2010)
  • Author(s): Moriyarna, T
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: Osaka
  • Year and Date: 2010-05-21
• [Presentation] A mobile nucleoporin, Nup98, plays an important role in a Crm1-dependent nuclear export (2009)
  • Author(s): 岡正啓
  • Organizer: 日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] importin αとNup153の相互作用は核内輸送を促進する (2009)
  • Author(s): 小川泰
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] A mobile nucleoporin, Nup98, plays an important role in a Crm1-dependent nuclear export (2009)
  • Author(s): 岡正啓
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] Interaction between importin alpha and Nup153 accelerates nuclear import (2009)
  • Author(s): 小川泰
  • Organizer: 2009 International Meeting on Nuclear Trafficking
  • Place of Presentation: Banff, Canada
• [Presentation] The role of Nup98 in a Crm1-mediated nuclear export (2008)
  • Author(s): 岡正啓
  • Organizer: CSHL meeting on Dynamic Organization of Nuclear Function
  • Place of Presentation: ニューヨーク/USA
  • Year and Date: 2008-09-19
• [Presentation] The role of Nup98 in a Crml-mediated nuclear export (2008)
  • Author(s): 岡 正啓
  • Organizer: CSHL meeting on Dynamic Organization of Nuclear Function
  • Place of Presentation: ニューヨーク/USA
  • Year and Date: 2008-09-19
• [Presentation] Nuclear pore dynamics during sleletal myogenesis (2008)
  • Author(s): 浅利 宗弘
  • Organizer: CSHL meeting on Dynamic Organization of Nuclear Function
  • Place of Presentation: ニューヨーク/USA
  • Year and Date: 2008-09-18
• [Presentation] Analysis of potential roles of Nup98 FG repeat region in oncogenesis (2008)
  • Author(s): 岡正啓
  • Organizer: 日本細胞生物学大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-07-01
• [Presentation] Analysis of potential roles of Nup98 FG repeat region in oncogenesis (2008)
  • Author(s): 岡 正啓
  • Organizer: 第60回 日本細胞生物学大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-07-01
• [Presentation] Functional differences between two isoforms of Npap60 (Nup50) in nuclear import (2008)
  • Author(s): 小川 泰
  • Organizer: 第60回 日本細胞生物学大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-29
• [Remarks] ホームページ等
• [Remarks] 大阪大学大学院・生命機能研究科ホームページ
  • URL: http://www.fbs.osaka-u.ac.jp/