Analysis of mitochondrial proteins required for regulation of mitochondrial morphology

• Project/Area Number: 20570185
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Kyushu University
• Principal Investigator: OKA Toshihiko Kyushu University, 医学研究院, 准教授 (40263321)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ミトコンドリア / 形態形成 / MICS1 / KLP6 / オルガネラ / オルガネラ形態

Research Abstract

To identify genes for regulation of mitochondrial morphology, we applied a feeding RNA interference in nematode C. elegans, and searched genes for mitochondrial proteins and molecular motors. We showed that MICS1 and KLP6 are required for mitochondrial morphology and transport, respectively.

Research Products

• [Journal Article] KLP6, a new kinesin regulating morphology and transport of mitochondria in neuronal cells. (2011)
  • Author(s): Tanaka, K., Sugiura, Y., Ichishita, R., Mihara, K., Oka, T.
  • Journal Title: J. Cell Sci. in press
  • Peer Reviewed
• [Journal Article] ミトコンドリア膜構造変化とアポトーシス制御 (2010)
  • Author(s): 大寺秀典、岡敏彦
  • Journal Title: 細胞工学 Volume: 29 Pages: 438-442
• [Journal Article] Identification of a novel protein MICS1 that is involved in maintenance of mitochondrial morphology and apoptotic release of cytochrome c. (2008)
  • Author(s): Oka, T., Sayano, T., Tamai, S., Yokota, S., Kato, H., Fujii, G., Mihara, K.
  • Journal Title: Mol. Biol. Cell 19 Pages: 2597-2608
  • Peer Reviewed
• [Journal Article] An RNAi screen for mitochondrial proteins required to maintain the morphology of the organelle in C. elegans. (2008)
  • Author(s): Ichishita, R., Tanaka, K., Sugiura, Y., Sayano, T., Mihara, K., Oka, T.
  • Journal Title: J. Biochem. 143 Pages: 449-454
  • Peer Reviewed
• [Journal Article] Identification of a novel protein MICSI that is involved in maintenance of mitochondrial morphology and apoptotic release of cytochrome c (2008)
  • Author(s): Oka, et al
  • Journal Title: Mol. Biol. Cell 19 Pages: 2597-2608
  • Peer Reviewed
• [Journal Article] An RNAi screen for mitochondrial proteins required to maintain the morphology of the organelle In C. elegans (2008)
  • Author(s): Ichishita, et al
  • Journal Title: J. Biochem 143 Pages: 449-454
  • Peer Reviewed
• [Journal Article] KLP6, a new kinesin regulating morphology and transport of mitochondria in neuronal cells
  • Author(s): Tanaka, et al
  • Journal Title: Journal of Cell Science Volume: (In press)
  • Peer Reviewed
• [Presentation] 日本生化学会・日本分子生物学会合同大会 (2010)
  • Organizer: 神戸ポートアイランド「ミトコンドリアの形態形成と軸索移動に関わる新規キネシンの機能解析」
  • Year and Date: 2010-12-09
• [Presentation] ミトコンドリアの形態形成と軸索移動に関わる新規キネシンの機能解析 (2010)
  • Author(s): 岡敏彦
  • Organizer: 日本生化学会日本分子生物学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] American Society of Cell Biology (2009)
  • Organizer: 米国サンディエゴコンベンションセンター「MICS1 is involved in maintenance of mitochondrial morphology and apoptotic release of cytochrome c」
  • Year and Date: 2009-12-06
• [Presentation] MICS1 is involved in maintenance of mitochondrial morphology and apoptotic release of cytochrome c (2009)
  • Author(s): 岡敏彦
  • Organizer: American Society of Cell Biology
  • Place of Presentation: 合衆国サンディエゴサンディエゴコンベンションセンター
  • Year and Date: 2009-12-06
• [Presentation] 日本蛋白質科学会 (2009)
  • Organizer: 熊本全日空ホテルニュースカイ「ミトコンドリアAAA蛋白質とその生理機能」
  • Year and Date: 2009-05-20
• [Presentation] ミトコンドリアAAA蛋白質とその生理機能 (2009)
  • Author(s): 岡敏彦
  • Organizer: 第9回日本蛋白質科学会
  • Place of Presentation: 熊本全日空ホテルニュースカイ
  • Year and Date: 2009-05-20
• [Presentation] 日本生化学会・日本分子生物学会合同大会 (2008)
  • Organizer: 神戸ポートアイランド「Regulation of mitochondrial morphology and cristae organization」
  • Year and Date: 2008-12-09
• [Presentation] Regulation of mitochondrial morphology and cristae organization (2008)
  • Author(s): 岡 敏彦
  • Organizer: 日本生化学会・日本分子生物学会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-09
• [Presentation] 日本細胞生物学会 (2008)
  • Organizer: パシフィコ横浜「新規ミトコンドリアタンパク質MICS1の機能解析」
  • Year and Date: 2008-06-29
• [Presentation] 新規ミトコンドリアタンパク質MICSIの機能解析 (2008)
  • Author(s): 岡 敏彦
  • Organizer: 日本細胞生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-06-29
• [Book] 細胞工学(「ミトコンドリア膜構造変化とアポトーシス制御」) (2010)
  • Author(s): 大寺秀典、岡敏彦