Study on the molecular mechanisms by which general cell polarity-regulating kinase, PAR-1, regulates epithelial polarity

• Project/Area Number: 20570186
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Yokohama City University
• Principal Investigator: SUZUKI Atsushi Yokohama City University, 医学研究科, 准教授 (00264606)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 細胞極性 / PAR / 上皮細胞 / 微小管 / aPKC

Research Abstract

Epithelial cells cover the boundary between our body and external environment, and thus develop functional asymmetry (polarity) between the cell membranes facing internal and external environment. It has been demonstrated that abnormal epithelial polarity leads to physiological deficiency. In this study, we have analyzed how the general cell polarity-regulating protein, PAR-1, plays important roles for epithelial polarity, especially by analyzing its binding proteins.

Research Products

• [Journal Article] KIBRA suppresses apical exocytosis through inhibition of aPKC kinase activity in epithelial cells. (2011)
  • Author(s): Yoshihama Y, Sasaki K, Horikoshi Y, Suzuki A, Ohtsuka T, Hakuno F, Takahashi S, Ohno S, Chida K.
  • Journal Title: Curr Biol 21 Pages: 705-711
  • Peer Reviewed
• [Journal Article] KIBRA Suppresses Apical Exocytosis through Inhibition of aPKC Kinase Activity in Epithelial Cells. (2011)
  • Author(s): Yoshihama, et al.
  • Journal Title: Curr.Biol. Volume: 21 Pages: 705-711
  • Peer Reviewed
• [Journal Article] Proliferation of human lung cancer in an orthotopic transplantation mouse model. (2010)
  • Author(s): Kan Y, Ohmura M, Suzuki A, Theeraladanon C, Oka T, Nakagami Y, Suzuki A, Nagashima Y, Inoue T.
  • Journal Title: Exp. Therapeu. Med. 1 Pages: 471-475
  • Peer Reviewed
• [Journal Article] The 8th and 9th tandem spectrin-like repeat of utrophin cooperatively form a functional unit to interact with polarity-regulating kinase Par-1b. (2010)
  • Author(s): Yamashita K, Suzuki A, Satoh Y. Ide M, Amano Y, Masuda-Hirata M, Hayashi Y, Hamada K, Ogata K, Ohno S.
  • Journal Title: BBRC 391 Pages: 812-817
  • Peer Reviewed
• [Journal Article] The 8th and 9th tandem spectrin-like repeat of utrophin cooperatively form a functional unit to interact with polarity-regulating kinase Par-1b. (2010)
  • Author(s): Yamashita K, et al.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 391 Pages: 812-817
  • Peer Reviewed
• [Journal Article] Proliferation of human lung cancer in an orthotopic transplantation mouse model (2010)
  • Author(s): Kan Y, et al.
  • Journal Title: Exp.Therapeu.Med. Volume: 1 Pages: 471-475
  • Peer Reviewed
• [Journal Article] An essential role of the aPKC-Aurora A-NDEL1 pathway on neurite elongation by modulation of microtubule dynamics. (2009)
  • Author(s): Mori D, Yamada M, Mimori-Kiyosue Y, Shirai Y, Suzuki A, Ohno S, Saya H, Wynshaw-Boris A, Hirotsune S.
  • Journal Title: Nat. Cell Biol. 11(9) Pages: 1057-1068
  • Peer Reviewed
• [Journal Article] Intracellular polarity protein PAR-1 regulates extracellular laminin assembly by regulating the dystroglycan complex. (2009)
  • Author(s): Masuda-Hirata. M, Suzuki A, Amano Y, Yamashita K, Ide M, Yamanaka T, Sakai M, Imamura M, Ohno S.
  • Journal Title: Genes to Cells 14 Pages: 835-850
  • Peer Reviewed
• [Journal Article] Interaction between PAR-3 and the aPKC-PAR-6 complex is indispensable for apical domain development of epithelial cells. (2009)
  • Author(s): Horikoshi Y, Suzuki A, Yamanaka T, Sasaki K, Mizuno K, Sawada H, Yonemura S, Ohno S.
  • Journal Title: J. Cell Sci. 122 Pages: 1595-1606
  • Peer Reviewed
• [Journal Article] The 8th and 9th tandem spectrin-like repeats of utrophin cooperatively form a functional unit to interact with polarity-regulating kinase PAR-1b (2009)
  • Author(s): Yamashita K, et al.
  • Journal Title: Biochem Biophys Res Commun 391 Pages: 812-817
  • Peer Reviewed
• [Journal Article] An essential role of the aPKC-Aurora A-NDEL1 pathway in neurite elongation by modulation of microtubule dynamics (2009)
  • Author(s): Mori D, et al.
  • Journal Title: Nat.Cell Biol. 11 Pages: 1057-1068
  • Peer Reviewed
• [Journal Article] Intracellular polarity protein PAR-1 regulates extracellular laminin assembly by regulating the dystroglycan complex (2009)
  • Author(s): Masuda-Hirata M, et al.
  • Journal Title: Genes Cells 14 Pages: 835-850
  • Peer Reviewed
• [Journal Article] Interaction between PAR-3 and the aPKC-PAR-6 complex is indispensable for apical domain development of epithelial cells (2009)
  • Author(s): Horikoshi Y, et al.
  • Journal Title: J.Cell Sci. 122 Pages: 1595-1606
  • Peer Reviewed
• [Journal Article] Affixin activates Rac1 via bPix in C2C12 myoblast. (2008)
  • Author(s): Matsuda C, Kameyama K, Suzuki A, Mishima W, Yamaji S, Okamoto H, Nishino I, Hayashi Y.
  • Journal Title: FEBS Lett 582 Pages: 1189-1196
  • Peer Reviewed
• [Journal Article] Regulation of epithelial and endothelial junctions by PAR proteins. (2008)
  • Author(s): Ebnet K, Iden S, Gerke V, Suzuki A.
  • Journal Title: Front Biosci 13 Pages: 6520-6536
  • Peer Reviewed
• [Journal Article] aPKC enables development of zonula adherens by antagonizing centripetal contraction of the circumferential actomyosin cables. (2008)
  • Author(s): Kishikawa M, Suzuki A, Ohno S.
  • Journal Title: J Cell Sci 121 Pages: 2481-2492
  • Peer Reviewed
• [Journal Article] Affixin activates Racl via beta PIX in C2C12 myoblast. (2008)
  • Author(s): Matsuda, et al.
  • Journal Title: FEBS Lett. 582 Pages: 1189-1196
  • Peer Reviewed
• [Journal Article] aPKC enables development of zonula adherens by antagonizing centripetal contraction of the circumferential actomyosin cables (2008)
  • Author(s): Kishikawa, et al.
  • Journal Title: J. Cell Sci. 121 Pages: 2481-2492
  • Peer Reviewed
• [Journal Article] Regulation of epithelial and endothelial junctions by PAR proteins. (2008)
  • Author(s): Ebnet, et al.
  • Journal Title: Front. Biosci. 13 Pages: 6520-6536
  • Peer Reviewed
• [Presentation] 細胞極性によるHippo経路の抑制がマウス着床前胚における位置依存的な細胞分化をつかさどる. (2010)
  • Author(s): 平手良和, 他
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 兵庫県 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] KIBRA suppresses apical exocytosis through inhibition of aPKC kinase activity inepithelial cells. (2010)
  • Author(s): 吉濱陽平, 他
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 兵庫県 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] Maintenance of dendritic spine morphology by PARIb through regulation of microtubule dynamics. (2010)
  • Author(s): 林健二, 他
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 兵庫県 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] 新規PAR1結合タンパク質p250は,微小管安定化タンパク質である (2010)
  • Author(s): 鈴木厚, 他
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪府 大阪国際会議場
  • Year and Date: 2010-05-20
• [Presentation] KIBRA suppresses apical exocytosis through inhibition of aPKC kinase activity in epithelial cells. (2010)
  • Author(s): Yoshihama Y, Sasaki K, Horokoshi Y, Ohtsuka T, Hakuno F, Takahashi S-I, Suzuki A, Ohno S, Chida K
  • Organizer: The 50th American Society for Cell Biology Annual Meeting
  • Place of Presentation: Philadelphia
• [Presentation] Maintenance of dendritic spine morphology by PAR1b through regulation of microtubule dynamics. (2010)
  • Author(s): 林健二, 鈴木厚, 大野茂男
  • Organizer: 第33回日本分子生物学会年会 第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
• [Presentation] KIBRA suppresses apical exocytosis through inhibition of aPKC kinase activity in epithelial cells. (2010)
  • Author(s): Yoshihama Y, Sasaki K, Horokoshi Y, Ohtsuka T, Hakuno F, Takahashi S-I, Suzuki A, Ohno S, Chida K
  • Organizer: 第33回日本分子生物学会年会 第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
• [Presentation] 細胞極性による Hippo 経路の抑制がマウス着床前胚における位置依存的な細胞分化をつかさどる (2010)
  • Author(s): 平手良和, 平原志乃, 井上健一, 中尾和貴, 鈴木厚, 秋本和憲, 大野茂男, 平井孝明, 千田和広, Marikawa Y, Vernadeth A, 佐々木洋
  • Organizer: 第33回日本分子生物学会年会 第83回日本生化学会大会 合同大会(口頭発表)
  • Place of Presentation: 神戸
• [Presentation] 新規PAR1 結合タンパク質p250 は,微小管安定化タンパク質である (2010)
  • Author(s): 鈴木厚, 佐藤由典, 秋津真志, 天野芳子, 大野茂男
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪市
• [Presentation] KIBRA suppresses apical exocytosis through in hibition of aPKC kinase activity in epithelial cells. (2010)
  • Author(s): Yoshihama Y, et al.
  • Organizer: The 50th American Society for Cell Biology Annual Meeting
  • Place of Presentation: 米国フィラデルフィアコンベンションセンター
• [Presentation] PAR-aPKCシステムによる上皮細胞極性制御機構 (2009)
  • Author(s): 鈴木厚
  • Organizer: 創剤フォーラム第15回シンポジウム
  • Place of Presentation: 東京都
  • Year and Date: 2009-10-23
• [Presentation] Intracellular polarity protein PAR-1 regulates extracellular laminin assembly by regulating the dystroglycan complex. (2009)
  • Author(s): A.Suzuki, K.Yamashita, Y.Amano, M. Masuda-Hirata, S.Ohno
  • Organizer: American Society for Cell Biology 49th Annual Meeting
  • Place of Presentation: San Diego, CA
• [Presentation] Interaction between PAR-3 and the aPKC-PAR-6 Complex Is Indispensable for Apical Domain Development of Epithelial Cells. (2009)
  • Author(s): 堀越洋輔, 鈴木厚, 山中智行, 佐々木和教, 水野恵子, 米村重信, 大野茂男
  • Organizer: The American Society for Cell Biology /Japan Society for Cell Biology /RIKEN Center for Developmental Biology Meeting
  • Place of Presentation: Kyoto
• [Presentation] PAR-1/MARK Regulates Extracellular Laminin Assembly by Controling the Dystroglycan Complex. (2009)
  • Author(s): A.Suzuki, K.Yamashita, Y.Amano, M. Hirata, S.Ohno
  • Organizer: The American Society for Cell Biology /Japan Society for Cell Biology /RIKEN Center for Developmental Biology Meeting
  • Place of Presentation: Kyoto
• [Presentation] 細胞内極性タンパク質,PAR-1 はジストログリカン複合体を制御することによって,細胞外のラミニンの集積を制御している (2009)
  • Author(s): 鈴木厚, 平田真樹, 天野芳子, 山下和成, 大野茂男
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] PAR-3 とaPKC-PAR-6 との相互作用は,上皮細胞のアピカルドメインの形成に必要である (2009)
  • Author(s): 堀越洋輔, 鈴木厚, 山中智行, 佐々木和教, 水野恵子, 米村重信, 大野茂男
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] 円柱様上皮細胞の形態に必須な新規PAR-1b 結合タンパク質 (2009)
  • Author(s): 秋津真志, 鈴木厚, 山下和成, 天野芳子, 井出真理子, 大野茂男
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] 細胞極性制御キナーゼPAR-1b は,utrophin のspectrin repeat ドメインと相互作用することで,utrophin- dystroglycan 複合体を安定化する (2009)
  • Author(s): 山下和成, 鈴木厚, 井出真理子, 天野芳子, 林由起子, 平田真樹, 嶋田香子, 大野茂男
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] Intracellular polarity protein PAR-1 regulates extracellular laminin assembly by regulating the dystroglycan complex (2009)
  • Author(s): 鈴木厚
  • Organizer: American Society for Cell Biology 49th Annual Meeting
  • Place of Presentation: San Diego
• [Presentation] Interaction between PAR-3 and the aPKC-PAR-6 Complex Is Indispensable for Apical Domain Development of Epithelial Cells (2009)
  • Author(s): 鈴木厚
  • Organizer: The American Society for Cell Biology /Japan Society for Cell Biology /RIKEN Center for Developmental Biology Meeting
  • Place of Presentation: Kyoto
• [Presentation] 円柱様上皮細胞の形態に必須な新規PAR-1b結合タンパク質 (2009)
  • Author(s): 秋津真志, ら
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] PAR-3とaPKC-PAR-6との相互作用は, 上皮細胞のアピカルドメインの形成に必要である (2009)
  • Author(s): 堀越洋輔, ら
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] PAR-1はジストログリカン複合体を制御することによって, 細胞外のラミニンの集積を制御している (2009)
  • Author(s): 鈴木厚, ら
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] aPKC は,円周性アクトミオシンバンドルの収縮をアンタゴナイズすることによって上皮細胞の接着結合の発達に必須な役割を果たしている (2008)
  • Author(s): 岸川優, 鈴木厚, 大野茂男
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜, 日本
• [Remarks] ホームページ等
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