The Interactive Mechanisms of neural cell adhesion molecules ; Protocadherin and IgCAM.

• Project/Area Number: 20570187
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Niigata University
• Principal Investigator: TAKEUCHI Kosei Niigata University, 医歯学系, 准教授 (90206946)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 脳・神経 / 神経回路 / 細胞接着 / 神経細胞接着 / タンパク質発現 / モデル動物 / 神経回路形成

Research Abstract

Immunoglobulin superfamily cell adhesion molecules (IgCAM) and Proto-cadherin family members (Pcdh) play important roles in the neural network formations. These member proteins have the interaction systems of hemophilic binding and heterophilic bindings to not only various cell adhesion proteins but also many extra cellular proteins (ECMs). IgCAMs and Pcdh were regulated by the expression of chondroitin sulfate proteoglycans especially. We generated the knockout mice of the enzymes involved in the synthesizing of chondroitin sulfate. The expression pattern of IgCAMs and Pcdh were perturbed in the developmental stages of brain formation.

Research Products

• [Journal Article] Igarashi M : Expression and function of neuronal growth-associated proteins(nGAPs)in PC12 cells. (2011)
  • Author(s): Lu J, Nozumi M, Takeuchi K, Abe H
  • Journal Title: Neurosci Res. 70 Pages: 85-90
  • Peer Reviewed
• [Journal Article] Predicted expansion of the claudin multigene family. (2011)
  • Author(s): Mineta K, Yamamoto Y, Yamazaki Y, Tanaka H, Tada Y, Saito K, Tamura A, Igarashi M, Endo T, Takeuchi K, Tsukita S
  • Journal Title: FEBS Lett. 585 Pages: 606-612
  • Peer Reviewed
• [Journal Article] Predicted exoansion of the claudin multigene family (2011)
  • Author(s): Mineta K., et.al
  • Journal Title: FEBS Volume: 585 Pages: 606-61
  • Peer Reviewed
• [Journal Article] Expression and function of neuronal growth-associated proteins (nGAPs)in PC12 cells. (2011)
  • Author(s): Lu J, Nozumi M, Takeuchi K, Igarashi M.
  • Journal Title: Neurosci Res. Volume: 70 Pages: 85-90
  • Peer Reviewed
• [Journal Article] Chondroitinsulfate N-acetylgalactosaminyl-transferase-1 is required for normal cartilage development. (2010)
  • Author(s): Takeuchi K., Watanabe Y., Higa-Onaga S., Sato M., Tsujita M., Abe M., Natsume R., Li M., Furuichi T., Saeki M., Izumikawa T., Hasegawa A., Yokoyama M., Ikegawa S., Sakimura K., Amizuka N., Kitagawa H, Igarashi M.
  • Journal Title: Biochem. J.Biochem. J. 432 Pages: 47-55
  • Peer Reviewed
• [Journal Article] Chondroitin sulfate N-acetylgalactosaminyltransferase-1 is required for normal cartilage development (2010)
  • Author(s): Takeuchi K., Watanabe Y, Higa-Onaga S., (以上equal contributes), et.al.
  • Journal Title: Biochem.J. Volume: 432 Pages: 47-55
  • Peer Reviewed
• [Journal Article] Identification of functional marker proteins in themammalian growth cone. (2009)
  • Author(s): Nozumi M., Togano T., Takahashi-Niki K., Lu J., Honda A., Taoka M., Shinkawa T., Koga H., Takeuchi K., Isobe T., Igarashi M.
  • Journal Title: Proc.Natl.Acad.Sci.USA 106 Pages: 17211-17216
  • Peer Reviewed
• [Journal Article] Structural basis for antiproliferative activity of Tob-hCaf1 complex. (2009)
  • Author(s): Horiuchi M., Takeuchi K., Noda N., Muroya N., Suzuki T., Nakamura T., Kawamura-Tsuzuku J., Takahashi K., Yamamoto T., Inagaki F.
  • Journal Title: J.Biol.Chem. 284 Pages: 13244-13255
  • Peer Reviewed
• [Journal Article] Identification of functional marker proteins in the mammalian growth cone (2009)
  • Author(s): Nozumi M., et. al
  • Journal Title: Proc.Natl.Acad.Sci.USA 106: Pages: 17211-17216
  • Peer Reviewed
• [Journal Article] Structural basis for antiproliferative activity of Tob-hCaf1 complex. (2009)
  • Author(s): Horiuchi M., et. al
  • Journal Title: J.Biol.Chem. 284 Pages: 13244-13255
  • Peer Reviewed
• [Journal Article] Structural basis for the antiproliferative activity of the Tob- h Caf1 complex. (2009)
  • Author(s): Horiuchi M, et. al.
  • Journal Title: J. Biol. Chem. 284
  • Peer Reviewed
• [Journal Article] Identification of glutamic acid decarboxylase gene and distribution of GABAergic nervous system in the planarian Dugesia iaponica. (2008)
  • Author(s): Nishimura K, et. al.
  • Journal Title: Neuroscience 153 Pages: 1103-1114
  • Peer Reviewed
• [Journal Article] Deficiency of ZO-1 causes embryonic lethal phenotype associated with defected yolk sac angiogenesis and apotosis of embryonic cells (2008)
  • Author(s): Katsuno T, et. al.
  • Journal Title: Mol. Bio1. Cell 19 Pages: 2465-2475
  • Peer Reviewed
• [Journal Article] Identification and distribution of tryptophan hydroxylase(TPH)-positive neurons in the planarian Dugesia japonica. (2008)
  • Author(s): Nishimura K, et. al.
  • Journal Title: Neurosci. Res. 59 Pages: 101-106
  • Peer Reviewed
• [Presentation] 細胞外基質コンドロイチン硫酸は如何に神経発生・神経再生に機能するか (2011)
  • Author(s): 武内恒成
  • Organizer: 第88回日本生理学会大会・第116回日本解剖学会総会全国学術集会合同大会
  • Place of Presentation: ハシフィコ横浜(大震災につき大会未開催も誌上開催とされ)
• [Presentation] Brain development abnormality in the mice lacking in the enzymes synthesizing chondroitin sulfate (2010)
  • Author(s): Susumu Higa Onaga, Yumi Watanabe, Kosei Takeuchi, Michihiro Igarashi
  • Organizer: SFN 2010
  • Place of Presentation: Washington, USA
  • Year and Date: 2010-09-03
• [Presentation] 新規バキュロウイルス発現細胞と膜タンパク質応用解析技術 (2010)
  • Author(s): 武内恒成、五十嵐道弘
  • Organizer: 新技術説明会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-30
• [Presentation] コンドロイチン硫酸合成酵素欠損マウスにおける脳発達異常 (2010)
  • Author(s): 比嘉進、武内恒成、小牟田縁、北川裕之、五十嵐道弘
  • Organizer: 第33回日本神経科学大会、日本神経化学会合同大会
  • Place of Presentation: 横浜
• [Presentation] Functional analyses of mice lacking an enzyme for chondroitin sulfate synthesis (2010)
  • Author(s): Kosei Takeuchi, Susumu Higa-Onaga, Hitoshi Kawano, Michihiro Igarashi
  • Organizer: 7^<th> Forum of European Neurosciences.
  • Place of Presentation: Amsterdam, Netherland
• [Presentation] 遺伝子導入からみた遺伝子発現と遺伝子発現抑制系 (2009)
  • Author(s): 武内恒成
  • Organizer: 第82回 日本化学会大会 シンポジウム
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2009-10-24
• [Presentation] プラナリアから探る神経系の進化:免疫グロブリンスーパーファミリー(IgSF)とカドヘリンスーパーファミリー(CdhSF)接着分子群の多様性 (2009)
  • Author(s): 峯田克彦、阿形清和、五十嵐道弘、武内恒成
  • Organizer: 第11回 日本進化学会大会 シンポジウム
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2009-10-24
• [Presentation] プラナリアから探る神経系の進化:免疫グロブリンスーパーファミリー(IgSF)とカドヘリンスーパーファミリー(CdhSF)接着分子群の多様性 (2009)
  • Author(s): 峯田克彦、阿形清和、五十嵐道弘、武内恒成
  • Organizer: 第11回日本進化学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2009-09-02
• [Presentation] 遺伝子導入からみた遺伝子発現と遺伝子発現抑制系 (2009)
  • Author(s): 武内恒成、五十嵐道弘
  • Organizer: 第82回日本生化学会大会シンポジウム
  • Place of Presentation: 神戸
• [Remarks] ホームページ等
• [Patent(Industrial Property Rights)] 無血清培養できるカイコ培養細胞株の作出およびその利用 (2009)
  • Inventor(s): 今西重雄・吉田芳哉・武内恒成
  • Industrial Property Rights Holder: 今西重雄・吉田芳哉・武内恒成
  • Industrial Property Number: 2009-200187
  • Filing Date: 2009-08-31