| Project/Area Number |
20570189
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Kyorin University |
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Principal Investigator |
MICA Ohara-Imaizumi Kyorin University, 医学部, 准教授 (40201941)
|
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| Co-Investigator(Kenkyū-buntansha) |
AOYAGI Kyota 杏林大学, 医学部, 助教 (50453527)
|
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| Co-Investigator(Renkei-kenkyūsha) |
NAGAMATSU Shinya 杏林大学, 医学部, 教授 (80231489)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | インスリン分泌 / 開口放出 / 糖尿病 / 全反射顕微鏡 / 細胞内トラフィック / インスリン |
|---|
| Research Abstract |
Insulin is stored in pancreaticβcell granules, and released biphasically by the exocytotic mechanism induced by nutrient glucose. In order to reveal the molecular mechanism of biphasic insulin granule exocytosis, we utilized a newly innovated imaging technique, TIRF-Confocal-Hybrid imaging system. Our results showed that there are two pools of insulin secretory granules docked at the plasma membrane and existed in the cytosol located ~500 nm from the cell surface. TIRF-Confocal-Hybrid imaging revealed that glucose stimulation evoked the translocation of insulin granules from inner pool to the plasma membrane, and fusion of granules with cell membrane was occurred after reaching the cell surface. Furthermore, the experiments using recombinant adenovirus encoding actin-EGFP treated βcells showed the close association of insulin granule intracellular movement with actin-network. Thus, TIRF-Confocal-Hybrid imaging is a valuabletechnique to reveal the mechanism of intracellular vesicle trafficking.
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