Organization of Endoplasmic reticulum exit sites

• Project/Area Number: 20570190
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: TANI Katsuko Tokyo University of Pharmacy and Life Science, 生命科学部, 教授 (40266896)
• Co-Investigator(Kenkyū-buntansha): ARIMITSU Nagisa 東京薬科大学, 生命科学部, 助教 (40408688)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 生体膜 / 小胞体 / 小胞輸送 / ノックアウトマウス / 精子形成 / ペルオキシソーム / ホスホリパーゼ / ミトコンドリア

Research Abstract

The ER exit site (ERES) is an ER subdomain, in which COPII-coated vesicles are formed. To elucidate the organization and functions of the ERES, we analyzed three components localized in the ERES, p125 and Sec16A and B. We generated p125-KO mice and found that p125 is required for spermiogenesis. We also demonstrated that Sec16B, but not Sec16A, is involved in the protein transport from the ER to peroxisomes.

Research Products

• [Journal Article] Golgi-localized KIAA0725p regulates membrane trafficking from the Golgi apparatus to the plasma membrane in mammalian cells. (2010)
  • Author(s): Sato S., Inoue H., Kogure T., Tagaya M., Tani K.
  • Journal Title: FEBS Lett. 584 Pages: 4389-4395
  • Peer Reviewed
• [Journal Article] Golgi-localized KIAA0725p regulates membrane trafficking from the Golgi apparatus to the plasma membrane in mammalian cells (2010)
  • Author(s): Sato, S., et al
  • Journal Title: FEBS Lett. Volume: 584 Pages: 4389-4395
  • Peer Reviewed
• [Journal Article] Role of syntaxin 18 in the organization of endoplasmic reticulum subdomains. (2009)
  • Author(s): Iinuma T., Aoki T., Arasaki K., Hirose H., Yamamoto A., Samata R., Hauri H.P., Arimitsu N., Tagaya M., Tani K.
  • Journal Title: J.CellSci. 122 Pages: 1680-1690
  • Peer Reviewed
• [Journal Article] UBXD1 is a VCP-interacting protein that is involved in ER-associated degradation. Biochem. (2009)
  • Author(s): Nagahama M., Ohnishi M., Kawate Y., Matsui T., Miyake H., Yuasa K, Tani K., Tagaya M., Tsuji A.
  • Journal Title: Biophys.Res.Commun. 382 Pages: 303-308
  • Peer Reviewed
• [Journal Article] Transcription arrest relief by S-II/TFIIS during gene expression in erythroblast differentiation. (2009)
  • Author(s): Nagata M., Ito T., Arimitsu N., Koyama H., Sekimizu K.
  • Journal Title: Genes Cells. 14 Pages: 371-80
  • Peer Reviewed
• [Journal Article] Identification of the neuroblastoma-amplified gene (NAG) product as a component of the syntaxin 18 complex implicated in Golgi-to-endoplasmic reticulum retrog rade transport. (2009)
  • Author(s): Nagahama, M., et al
  • Journal Title: Biochem.Biophys.Res.Commun 382 Pages: 303-308
  • Peer Reviewed
• [Journal Article] Role of syntaxin 18 in the organization of endoplasmic reticulum subdomains. (2009)
  • Author(s): Iinuma, T., et al
  • Journal Title: J.Cell Sci. 122 Pages: 1680-1690
  • Peer Reviewed
• [Journal Article] UBXDl is a VCP-interacting protein that is involved in ER-associated degradation. (2009)
  • Author(s): Nagahama, M., et al.
  • Journal Title: Biochem. Biophys. Res. Commun. 382 Pages: 303-308
  • Peer Reviewed
• [Journal Article] Adaptation of endoplasmic reticulum exit sites to acute and chronic increases in cargo load. (2008)
  • Author(s): Farhan H., Weiss M., Tani K., Kaufman R.J., Hauri H.P.
  • Journal Title: EMBO J. 27 Pages: 2043-2054
  • Peer Reviewed
• [Journal Article] Bap31 is an itinerant protein that moves between the peripheral endoplasmic reticulum (ER) and a juxtanuclear compartment related to ER-associated degradation. (2008)
  • Author(s): Wakana Y., Takai S., Nakajima K., Tani K., Yamamoto A., Watson P., Stephens D., Hauri H.-P., Tagaya M.
  • Journal Title: Mol.Biol.Cell 19 Pages: 1825-1836
  • Peer Reviewed
• [Journal Article] Adaptation of endoplasmic reticulum exit sites to acute and chronic in creases in cargo load. (2008)
  • Author(s): Farhan, H., et al.
  • Journal Title: EMBO J. 27 Pages: 2043-2054
  • Peer Reviewed
• [Journal Article] p125/Sec23-interacting protein (Sec23ip) is required for spermiogenesis.
  • Author(s): Arimitsu N., Kogure T., Baba T., Nakao K., Hamamoto H., Sekimizu K., Yamamoto A., Nakanishi H., Taguchi R., Tagaya M., Tani K.
  • Journal Title: FEBS Lett (in press)
  • Peer Reviewed
• [Presentation] 小胞体出芽部位局在タンパク質iPLA1-β/p125の精子形成への関与 (2010)
  • Author(s): 木榑猛、有光なぎさ、中尾和貴、浜本洋、関水和久、山本章嗣、多賀谷光男、谷佳津子
  • Organizer: 第62回日本細胞生物学大会
  • Place of Presentation: 大阪
• [Presentation] KIAA0725p/DDHD2, a mammalian Golgi-associated phospholipase A_1, regulates membrane trafficking from the Golgi apparatus to the plasma membrane. (2010)
  • Author(s): Inoue H., Sato S., Kogure T., Arimitsu N., Tagaya M., Tani K.
  • Organizer: Sapporo International Cancer Symposium 2010 "Membrane traffic and Cancer"
  • Place of Presentation: Sapporo
• [Presentation] KIAA0725p/DDHD2, a mammalian Golgi-associated phospholipase A1, regulates membrane trafficking from the Golgi apparatus to the plasma membrane. (2010)
  • Author(s): Inoue, H., Sato, S., Kogure, T., Arimitsu, N., Tagaya, M., Tani, K.
  • Organizer: Sapporo International Cancer Symposium 2010 "Membranetraffic and Cancer"
  • Place of Presentation: Sapporo
• [Presentation] PA-PLA1/iPLA1αの精子形成過程における発現時期の同定 (2009)
  • Author(s): 馬場崇, 他
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-22
• [Presentation] Sec16Bはミトコンドリアおよびペルオキシソームの形態形成に関与する (2009)
  • Author(s): 米川周佑, 他
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-22
• [Presentation] PA-PLA1/iPLA1αの精子形成過程における発現時期の同定 (2009)
  • Author(s): 馬場崇、有光なぎさ、多賀谷光男、谷佳津子
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] Sec16Bはミトコンドリアおよびペルオキシソームの形態形成に関与する (2009)
  • Author(s): 米川周佑、中島寿基、井上弘樹、多賀谷光男、谷佳津子
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] NESH(Abi-3)を含むWAVE複合体の解析 (2008)
  • Author(s): 中村 昌, 他
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-10
• [Presentation] NESH(Abi-3)を含むWAVE複合体の解析 (2008)
  • Author(s): 金沢仁志、中村昌、佐藤精一、谷佳津子
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Book] 機能性DDSキャリアの製剤設計 (2008)
  • Author(s): 谷 佳津子, 多賀谷光男, 他
  • Total Pages: 321
  • Publisher: シーェムシー出版
• [Book] Adaptor proteins and cancer (2008)
  • Author(s): Shishido, T, et al.
  • Total Pages: 218
  • Publisher: Transworld Research Network
• [Remarks] ホームページ等
  • URL: http://www.ls.toyaku.ac.jp/?cellsig/index.html
• [Remarks]
  • URL: http://www.ls.toyaku.ac.jp/~cellsig/index.html