Analyses of the fine-tuning mechanism of Arf6 activity on reorganization of epithelial organ

• Project/Area Number: 20570192
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Ehime University
• Principal Investigator: YANO Hajime Ehime University, 大学院・医学系研究科, 准教授 (00284414)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: Arf6 / 神経膠腫浸潤 / 浸潤抑制剤 / コンドロイチン硫酸プロテオグリカンNG2 / 神経膠腫 / ナトリウム/プロトン交換輸送体1(NHE1) / 神経膠腫細胞 / 細胞浸潤性亢進 / 神経膠腫細胞浸潤関連新規因子 / 神経膠腫細胞特異的発現亢進 / AMAP1 / 転写後調節 / 遺伝子変異

Research Abstract

Arf6 was previously proposed to be critical for epithelial cancer invasion. In this study, possible participation of one of the chondroitin sulfate proteoglycan NG2 at the upstream of Arf6 in glioma invasion was presented. Moreover, a signal mediated by sodium ion/proton exchanger 1 (NHE-1) was found to play a pivotal role in glioma cell invasion, independently of the Arf6 signal. These results implicate simultaneous inhibition of the both signals as effective therapeutic protocol for anti-invasion therapy of glioma.

Research Products

• [Journal Article] Subcutaneous injection containing IL-3 and GM-CSF ameliorates stab wound-induced brain injury in rats. (2011)
  • Author(s): Nishihara, T., et al.
  • Journal Title: Experimental Neurology In press
  • Peer Reviewed
• [Journal Article] Subcutaneous injection containing IL-3 and GM-CSF ameliorates stab wound-induced brain injury in rats (2011)
  • Author(s): Nishihara, T., et al.
  • Journal Title: Experimental Neurology Volume: (In press)
  • Peer Reviewed
• [Journal Article] Cancer stem-like cells of glioblastoma characteristically express MMP-13 and display highly invasive activity. (2010)
  • Author(s): Inoue, A., et al.
  • Journal Title: International Journal of Oncology 37 Pages: 1121-1131
  • Peer Reviewed
• [Journal Article] Iba1+/NG2+ macrophage-like cells expressing a variety of neuroprotective factors ameliorate ischemic damage of the brain. (2010)
  • Author(s): Smirkin, A., et al.
  • Journal Title: Journal of Cereberal Blood Flow and Metabolism 30 Pages: 603-615
  • Peer Reviewed
• [Journal Article] Cancer stem-like cells of glioblastoma characteristically express MMP-13 and display highly invasive activity. (2010)
  • Author(s): Inoue, A., et al.
  • Journal Title: International Journal of Oncology Volume: 37 Pages: 1121-1131
  • Peer Reviewed
• [Journal Article] Iba1+/NG2+ macrophage-like cells expressing a variety of neuroprotective factors ameliorate ischemic damage of the brain. (2010)
  • Author(s): Smirkin, A., et al.
  • Journal Title: Journal of Cereberal Blood Flow and Metabolism Volume: 30 Pages: 603-615
  • Peer Reviewed
• [Journal Article] Ibal+/NG2+ macrophage-like cells expressingariety of neuroprotective factors a to ischemic damage of the brain (2010)
  • Author(s): Smirkin, A., et at.
  • Journal Title: Journal of Cerebral Blood Flow & Metabolism 30 Pages: 603-615
  • Peer Reviewed
• [Journal Article] The EGFR-GEP100-Arf6-AMAP1 signaling pathway specific to breast cancer invasion and metastasis. (2009)
  • Author(s): Sabe, et al.
  • Journal Title: Traffic 10 Pages: 982-993
  • Peer Reviewed
• [Journal Article] The EGFRGEP100-Arf6-AMAP1 signaling pathway specific to breast cancer inv metastasis (2009)
  • Author(s): Sabe, H., et al.
  • Journal Title: Traffic 10 Pages: 982-993
  • Peer Reviewed
• [Presentation] 神経膠腫細胞浸潤性におけるNa^+/H^+ Exchanger (NHE1)の寄与について (2010)
  • Author(s): 矢野元, 他
  • Organizer: 日本生理学会中四国地方会
  • Place of Presentation: 出雲市
  • Year and Date: 2010-11-21
• [Presentation] 神経膠腫細胞浸潤性におけるNa^+/H^+ Exchanger(NHE1)の寄与について (2010)
  • Author(s): 矢野元, ほか
  • Organizer: 日本生理学会中四国地方会
  • Place of Presentation: 出雲市
  • Year and Date: 2010-11-21
• [Presentation] コンドロイチン硫酸プロテオグリカンNG2の細胞浸潤における役割 (2010)
  • Author(s): 矢野元, 他
  • Organizer: グリア研究会
  • Place of Presentation: 福岡市
  • Year and Date: 2010-10-23
• [Presentation] Invasive potential of astrocytes and disinhibition of the properties in gliomas. (2010)
  • Author(s): 矢野元, 他
  • Organizer: 日本神経科学会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-09-19
• [Presentation] Roles of NG2 in glioma cell invasion (2010)
  • Author(s): 河村智宏, 他
  • Organizer: 日本神経科学会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-09-19
• [Presentation] Roles of NG2 in glioma cell invasion. (2010)
  • Author(s): 河村智宏, 他
  • Organizer: 日本神経科学会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-09-04
• [Presentation] Various modes of glioma cell invasions and a possible common molecular machinery among the invasions. (2010)
  • Author(s): 矢野元, 他
  • Organizer: 日本生理学会
  • Place of Presentation: 盛岡市
  • Year and Date: 2010-05-19
• [Presentation] Dependency and independency of glioma cell invasions on Arf6 which is an essential factor for breast cancer cell invasion. (2009)
  • Author(s): 矢野元, 他
  • Organizer: 国際生理学会
  • Place of Presentation: 京都市
  • Year and Date: 2009-07-28
• [Presentation] Dependency and independency of glioma cell invasions on Arf6 whissential factor for breast cancer cell invasion (2009)
  • Author(s): 矢野元, 他
  • Organizer: 国際生理学会
  • Place of Presentation: 京都市
  • Year and Date: 2009-07-28
• [Book] メンブレントラフィックの奔流 (「第II章メンブレントラフィックと高次機能」のうち、『Arf6による細胞機能制御』) (2009)
  • Author(s): 矢野 元
  • Total Pages: 7
  • Publisher: 共立出版
• [Book] 培養細胞実験ハンドブック (「第5章培養細胞の解析」のうち『細胞運動観察法』) (2008)
  • Author(s): 矢野 元 佐邊 壽孝
  • Total Pages: 5
  • Publisher: 羊土社
• [Patent(Industrial Property Rights)] 浸潤抑制剤 (2010)
  • Inventor(s): 矢野元
  • Industrial Property Rights Holder: 愛媛大学
  • Industrial Property Number: 2010-100248
  • Filing Date: 2010-04-23