Analysis of neural and blood vessel network development in zebrafish mutant

• Project/Area Number: 20570195
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Tohoku University
• Principal Investigator: SHOJI Wataru 東北大学, 加齢医学研究所, 助教 (40250831)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 器官形成 / 神経 / 血管 / ゼブラフィッシュ / 神経発生 / 血管形成

Research Abstract

We identified zebrafish mutant that has defect in blood vessels and neural network development. The mutated gene encodes an enzyme on cholesterol synthesis, and geranyl-geranyl products, which derive from intermediate metabolite of this pathway, was proved to be essential for blood vessels and neural development. Prenyl modification activates numbers of small G proteins, and we found rab family members are important in blood vessel development. We are further investigating what and how specific members of this gene family are involved in blood vessel development.

Research Products

• [Journal Article] Islet1 selectively promotes peripheral axon outgrowth in Rohon-Beard primary sensory neurons (2011)
  • Author(s): Tanaka, H., Nojima, Y., Shoji, W., Sato, M., Nakayama, R., Ohshima, T., Okamoto, H
  • Journal Title: Developmental. Dynamics Volume: 240 Pages: 9-22
  • Peer Reviewed
• [Journal Article] Crucial role of peroxiredoxin III in placental antioxidant defense of mice (2008)
  • Author(s): Li, L., Shoji, W., Oshima, H., Obinata, M., Fukumoto, M., Kanno, N
  • Journal Title: FEBS Lett Volume: 582 Pages: 2431-2434
  • Peer Reviewed
• [Journal Article] Application of heat shock promoter in transgenic zebrafish (2008)
  • Author(s): Shoji, W. and Sato-Maeda, M
  • Journal Title: Dev Growth Differ Volume: 50 Pages: 401-406
  • NAID: 10021096963
  • Peer Reviewed
• [Journal Article] Position Fine-Tuning of Caudal Primary Motor Neurons in Zebrafish Spinal Cord (2008)
  • Author(s): Sato-Maeda, M., Obinata, M., and Shoji, W
  • Journal Title: Development Volume: 135 Pages: 323-332
  • Peer Reviewed
• [Journal Article] Application of heat shock promoter in transgenic zebrafish (2008)
  • Author(s): Shoji, W. and Sato-Maeda, M
  • Journal Title: Dev Growth Differ 50 Pages: 401-406
  • NAID: 10021096963
  • Peer Reviewed
• [Journal Article] Crucial role of peroxiredoxin III in placental antioxidant defense of mice (2008)
  • Author(s): Li, L., Shoji, W., Oshima, H., Obinata, M., Fukumoto, M., Kanno, N.
  • Journal Title: FEBS Lett 582 Pages: 2431-2434
  • Peer Reviewed
• [Presentation] 神経冠細胞と運動神経軸索との相互作用が、脊髄内・外のインターフェースの保全に果たすについて (2011)
  • Author(s): 前田(佐藤)美香、東海林亙
  • Organizer: 第135回東北大学加齢医学研究所・集団会
  • Place of Presentation: 仙台市
  • Year and Date: 2011-01-28
• [Presentation] ゼブラフィッシュ胚における一次運動神経細胞体を脊髄に保持する機構について (2010)
  • Author(s): 前田(佐藤)美香、東海林亙
  • Organizer: 第81回日本動物学会年会
  • Place of Presentation: 東京都
  • Year and Date: 2010-09-23
• [Presentation] ゼブラフィッシュ胚における一次運動神経細胞体を脊髄に保持する機構について (2010)
  • Author(s): 前田(佐藤)美香、東海林亙
  • Organizer: 第81回日本動物学会年会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-09-23
• [Presentation] neural crest-derived cells are involoved with earyly mechanisms to maintain motoneuron cell bodies in zebrafish embryonic spinal cord (2010)
  • Author(s): Sato-Meda, M. and Shoji, W
  • Organizer: 第43回日本発生生物学会年会
  • Place of Presentation: 京都市
  • Year and Date: 2010-06-21
• [Presentation] neural crest-derived cells are involoved with earyly mechanisms to maintain motoneuron cell bodies in zebrafish embryonic spinal cord (2010)
  • Author(s): Sato-Meda, M., Shoji, W.
  • Organizer: 第43回日本発生生物学会年会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-06-21
• [Presentation] Transgenic zebrafish expressing an optimized channelrhodopsin variant under regulation of Gal4/UAS systems : optogenetic stimulation of Rohon-Beard neurons (2010)
  • Author(s): 梅田恵子、東海林亙、石塚徹、八尾寛
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市
  • Year and Date: 2010-05-19
• [Presentation] ransgenic zebrafish expressing an optimized channelrhodopsin variant under regulation of Gal4/UAS systems : optogenetic stimulation of Rohon-Beard neurons (2010)
  • Author(s): 梅田恵子、東海林亙、石塚徹、八尾寛
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 岩手県盛岡市
  • Year and Date: 2010-05-19
• [Presentation] Neural crest-derived cells maintain motor neuron cell bodies in zebrafish embryonic spinal cord (2009)
  • Author(s): 前田(佐藤)美香、東海林亙
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-12-11
• [Presentation] Neural crest-derived cells maintain motor neuron cell bodies in zebrafish embryonic spinal cord (2009)
  • Author(s): 前田(佐藤)美香、東海林亙
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2009-12-11
• [Presentation] 神経細胞特異的に改変型チャネルロドプシンを発現しているトランスジェニックゼブラフィッシュの運動機能評価 (2009)
  • Author(s): 梅田恵子、東海林亙、石塚徹、八尾寛
  • Organizer: 第42回東北生理談話会
  • Place of Presentation: 盛岡市
  • Year and Date: 2009-10-17
• [Presentation] 神経細胞特異的に改変型チャネルロドプシンを発現しているトランスジェニックゼブラフィッシュの運動機能評価 (2009)
  • Author(s): 梅田恵子、東海林亙、石塚徹、人尾寛
  • Organizer: 第42回東北生理談話会
  • Place of Presentation: 岩手県盛岡市
  • Year and Date: 2009-10-17
• [Presentation] ゼブラフィッシュ運動神経細胞体の脊髄からの脱落現象について (2009)
  • Author(s): 前田(佐藤)美香、東海林亙
  • Organizer: 第80回日本動物学会年会
  • Place of Presentation: 静岡市
  • Year and Date: 2009-09-17
• [Presentation] ゼブラフィッシュ運動神経細胞体の脊髄からの脱落現象について (2009)
  • Author(s): 前田(佐藤)美香、東海林亙
  • Organizer: 第80回日本動物学会年会
  • Place of Presentation: 静岡県静岡市
  • Year and Date: 2009-09-17
• [Presentation] GAL4-UAS制御下に改変型チャネルロドプシンを発現するトランスジェニックゼブラフィッシュの作製とその評価 (2009)
  • Author(s): 梅田恵子、東海林亙、石塚徹、八尾寛
  • Organizer: 第15回小型魚類研究会
  • Place of Presentation: 名古屋市
  • Year and Date: 2009-09-12
• [Presentation] ゼブラフィッシュ胚・運動神経細胞体の脊髄からの脱落現象 (2009)
  • Author(s): 前田(佐藤)美香、東海林亙
  • Organizer: 第15回小型魚類研究会
  • Place of Presentation: 名古屋市
  • Year and Date: 2009-09-12
• [Presentation] GAL4-UAS制御下に改変型チャネルロドプシンを発現するトランスジェニックゼブラフィッシュの作製とその評価 (2009)
  • Author(s): 梅田恵子、東海林亙、石塚徹、八尾寛
  • Organizer: 第15回小型魚類研究会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2009-09-12
• [Presentation] ゼブラフィッシュ胚・運動神経細胞体の脊髄からの脱落現象 (2009)
  • Author(s): 前田(佐藤)美香、東海林亙
  • Organizer: 第15回小型魚類研究会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2009-09-12
• [Presentation] コレステロール代謝経路と発生異常 (2009)
  • Author(s): 東海林亙
  • Organizer: 第6回東北大学バイオサイエンスシンポジウム・第14回学際ライフサイエンスシンポジウム(シンポジウム講演)
  • Place of Presentation: 仙台市
  • Year and Date: 2009-06-16
• [Presentation] コレステロール代謝経路と発生異常 (2009)
  • Author(s): 東海林亙
  • Organizer: 第14回学際ライフサイエンスシンポジウム
  • Place of Presentation: 宮城県仙台市
  • Year and Date: 2009-06-16
• [Presentation] 脂質代謝と発生異常 (2009)
  • Author(s): 東海林亙
  • Organizer: 2009 NIG Zebrafish meeting
  • Place of Presentation: 三島市
  • Year and Date: 2009-03-18
• [Presentation] ゼブラフィッシュに見る脊髄運動神経細胞の規則的な配列とその形成機構 (2008)
  • Author(s): 東海林亙
  • Organizer: 応用数学連携フォーラム第6回ワークショップ
  • Place of Presentation: 仙台市
  • Year and Date: 2008-10-22
• [Presentation] Generation of iterative motor nerve pattern in zebrafish spinal cord, Frontiers in Developmental Biology (2008)
  • Author(s): Sato-Maeda, M., and Shoji, W
  • Organizer: Joint meeting of the French and Japanese Societies for Developmental Biology
  • Place of Presentation: Giens(フランス)
  • Year and Date: 2008-09-13
• [Presentation] Generation of iterative motor nerve pattern in zebrafish spinal cord (2008)
  • Author(s): Sato-Maeda, M., and Shoii, W.
  • Organizer: Frontiers in Developmental Biology, Joint meeting of the French and Japanese Societies for Developmental Biology
  • Place of Presentation: Giens(フランス)
  • Year and Date: 2008-09-13
• [Presentation] Generation of iterative motor nerve pattern in zebrafish spinal cord, Axon guidance (2008)
  • Author(s): Sato-Maeda, M., and Shoji, W
  • Organizer: Synaptogenesis & Neural plasticity in Cold Spring Harbor Laboratory
  • Place of Presentation: Cold Spring Harbor(米国)
  • Year and Date: 2008-09-10
• [Presentation] Generation of iterative motor nerve pattern in zebrafish spinal cord (2008)
  • Author(s): Sato-Maeda, M., and Shoji, W.
  • Organizer: Axon guidance, Synaptogenesis & Neural plasticity in Cold Spring Harbor Laboratory
  • Place of Presentation: Cold Spring Harbor(米国)
  • Year and Date: 2008-09-10
• [Presentation] ゼブラフィッシュ一次運動神経における細胞体の位置調整 (2008)
  • Author(s): 前田(佐藤)美香、東海林亙
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡市
  • Year and Date: 2008-09-06
• [Presentation] Positional adustment of zebrafish primary motoneurons before axonogenesis (2008)
  • Author(s): Sato-Maeda, M., Obinata, M., and Shoji, W
  • Organizer: 第41回日本発生生物学会
  • Place of Presentation: 徳島市
  • Year and Date: 2008-05-28
• [Remarks]