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Mechanisms in polarity formation and asymmetric division of angiospermic zygotes

Research Project

Project/Area Number 20570206
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionTokyo Metropolitan University

Principal Investigator

OKAMOTO Takashi  Tokyo Metropolitan University, 大学院・理工学研究科, 准教授 (50285095)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords受精 / 卵細胞 / 精細胞 / 受精卵 / 胚発生 / 細胞極性 / イネ
Research Abstract

In angiosperms, a zygote generally divides into an asymmetric two-celled embryo consisting of an apical and a basal cell with different cell fates, and this unequal division is a putative first step for formation of the apical?basal axis of plants. In the project, apical and basal call specific genes were identified using rice in vitro fertilization system and single cell transcriptome analyses. In addition, preparation of transformed rice plants for visualizing actin filament made it possible to observe dynamics of actin filament during zygote development.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (37 results)

All 2011 2010 2009 2008 Other

All Journal Article (20 results) (of which Peer Reviewed: 13 results) Presentation (12 results) Book (2 results) Remarks (3 results)

  • [Journal Article] In vitro fertilization with isolated rice gametes : production of zygotes and zygote and embryo culture.2011

    • Author(s)
      Okamoto T.
    • Journal Title

      Methods Mol.Biol. 710

      Pages: 17-27

    • Related Report
      2010 Final Research Report
  • [Journal Article] Distinct Gene Expression Profiles in Egg and Synergid Cells of Rice as Revealed by Cell Type-specific Microarrays.2011

    • Author(s)
      Ohnishi T., Takanashi H., Mogi M., Takahashi H., Kikuchi H., Yano K., Okamoto T., Fujita M., Kurata N., Tsutsumi N.
    • Journal Title

      Plant Physiol. 155

      Pages: 881-891

    • Related Report
      2010 Final Research Report
  • [Journal Article] In vitro fertilization with isolated rice gametes : production of zygotes and zygote and embryo culture.2011

    • Author(s)
      Okamoto T.
    • Journal Title

      Methods in Molecular Biology

      Volume: 710 Pages: 17-27

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distinct Gene Expression Profiles in Egg and Synergid Cells of Rice as Revealed by Cell Type-specific Microarrays.2011

    • Author(s)
      Ohnishi T., Takanashi H., Mogi M., Takahashi H., Kikuchi H., Yano K., Okamoto T., Fujita M., Kurata N., Tsutsumi N.
    • Journal Title

      Plant Physiology

      Volume: 155 Pages: 881-891

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gamete fusion site on the egg cell and autonomous establishment of cell polarity in the zygote.2010

    • Author(s)
      Okamoto T.
    • Journal Title

      Plant Signaling & Behavior 5

      Pages: 1464-1467

    • NAID

      130006993155

    • Related Report
      2010 Final Research Report
  • [Journal Article] Positional relationship between the gamete fusion site and the first division plane in the rice zygote.2010

    • Author(s)
      Nakajima K., Uchiumi T., Okamoto T.
    • Journal Title

      J.Exp.Bot. 61

      Pages: 3101-3105

    • Related Report
      2010 Final Research Report
  • [Journal Article] Asymmetric cell division of rice zygotes located in embryo sac and produced by in vitro fertilization.2010

    • Author(s)
      Sato A., Toyooka K., Okamoto T.
    • Journal Title

      Sex Plant Reprod. 23

      Pages: 211-217

    • Related Report
      2010 Final Research Report
  • [Journal Article] Studies of mitochondrial morphology and DNA amount in the rice egg cell.2010

    • Author(s)
      Takanashi, H., Ohnishi, T., Mogi, M., Okamoto, T., Arimura, S, Tsutsumi N.
    • Journal Title

      Curr.Genet. 56

      Pages: 33-41

    • Related Report
      2010 Final Research Report
  • [Journal Article] Gamete fusion site on the egg cell and autonomous establishment of cell polarity in the zygote.2010

    • Author(s)
      Okamoto T.
    • Journal Title

      Plant Signaling & Behavior

      Volume: 5 Pages: 1464-1467

    • NAID

      130006993155

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Positional relationship between the gamete fusion site and the first division plane in the rice zygote.2010

    • Author(s)
      Nakajima K., Uchiumi T., Okamoto T.
    • Journal Title

      Journal of Experimrental Botany

      Volume: 61 Pages: 3101-3105

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Asymmetric cell division of rice zygotes located in embryo sac and produced by in vitro fertilization.2010

    • Author(s)
      Sato A., Toyooka K., Okamoto T.
    • Journal Title

      Sexual Plant Reproduction

      Volume: 23 Pages: 211-217

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rice fruit development is associated with an increased IAA content in pollinated ovaries.2010

    • Author(s)
      Uchiumi T., Okamoto T.
    • Journal Title

      Planta

      Volume: 232 Pages: 579-592

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Asymmetric cell division of rice zygotes located in embryo sac and produced by in vitro fertilization.2010

    • Author(s)
      Sato A., Toyooka K., Okamoto T.
    • Journal Title

      Sexual Plant Reproduction (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro fertilization with isolated rice gametes : production of zygotes and zygote and embryo culture.2010

    • Author(s)
      Okamoto T.
    • Journal Title

      Methods in Molecular Biology (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Studies of mitochondrial morphology and DNA amount in the rice egg cell.2010

    • Author(s)
      Takanashi, H., Ohnishi, T., Mogi, M., Okamoto, T., Arimura, S., Tsutsumi N.
    • Journal Title

      Current Genetics Vol.56

      Pages: 33-41

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of polypyrimidine tract-binding protein (PTB) related proteins in pollen germination in Arabidopsis.2009

    • Author(s)
      Wang S., Okamoto T.
    • Journal Title

      Plant and Cell Physiology Vol.50

      Pages: 179-190

    • NAID

      10027336919

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro fertilization with isolated rice gametes : production of zygotes and zygote and embryo culture.2009

    • Author(s)
      Okamoto T.
    • Journal Title

      Methods Mol. Biol. (印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of polypyrimidine tract-binding protein (PTB) related proteins in pollen germination in Arabidopsis.2009

    • Author(s)
      Wang S., Okamoto T.
    • Journal Title

      Plant Cell Physiol. 50

      Pages: 179-190

    • NAID

      10027336919

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro fertilization with isolated higher plant gametes.2008

    • Author(s)
      Kranz E., Hoshino Y., Okamoto T.
    • Journal Title

      Methods Mol.Biol. 427

      Pages: 51-69

    • Related Report
      2010 Final Research Report
  • [Journal Article] In vitro fertilization with isolated higher plant gametes.2008

    • Author(s)
      Kranz E., Hoshino Y., Okamoto T
    • Journal Title

      Methods Mol. Biol. 427

      Pages: 51-69

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] イネ卵細胞膜上における配偶子融合領域と受精卵の自律的細胞内極性形成2011

    • Author(s)
      岡本龍史、佐藤明子、中島啓介
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2011-03-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] イネ雌雄配偶子および受精卵のトランスクリプトーム解析2011

    • Author(s)
      安彦真文、岡本龍史
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2011-03-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] イネ卵細胞膜上における配偶子融合領域と受精卵の自律的細胞内極性形成2011

    • Author(s)
      岡本龍史、佐藤明子、中島啓介
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Related Report
      2010 Final Research Report
  • [Presentation] イネ雌雄配偶子および受精卵のトランスクリプトーム解析2011

    • Author(s)
      安彦真文、岡本龍史
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Related Report
      2010 Final Research Report
  • [Presentation] イネ2細胞胚の頂端細胞-基部細胞間で差位的発現を示す遺伝子の同定2010

    • Author(s)
      岡本龍史、佐藤明子、豊岡公徳、内海貴夫
    • Organizer
      第51回日本植物生理学会
    • Place of Presentation
      熊本大学(熊本)
    • Year and Date
      2010-03-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] イネ2細胞胚の頂端細胞-基部細胞間で差位的発現を示す遺伝子の同定2010

    • Author(s)
      岡本龍史、佐藤明子、豊岡公徳、内海貴夫
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Related Report
      2010 Final Research Report
  • [Presentation] イネ雌性配偶体構成細胞における遺伝子発現プロファイリング2010

    • Author(s)
      高梨秀樹、大西孝幸、茂木美来、菊池俊介、矢野健太郎、岡本龍史、藤田雅丈、倉田のり、堤信浩
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Related Report
      2010 Final Research Report
  • [Presentation] 被子植物における配偶子融合の分子認証機構2009

    • Author(s)
      岡本龍史
    • Organizer
      第82回日本生化学会大会シンポジウム「動植物におけるアロ認証機構」
    • Place of Presentation
      神戸ポートビアホテル(神戸)
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] イネin vitro結実系を用いた初期果実生長の解析2009

    • Author(s)
      内海貴夫、岡本龍史
    • Organizer
      日本植物学会第73回大会
    • Place of Presentation
      山形大学(山形)
    • Year and Date
      2009-09-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] ポリピリミジン領域結合タンパク質(PTB)の花粉発芽への関与2009

    • Author(s)
      Wang Shuyi、岡本龍史
    • Organizer
      日本植物学会第73回大会
    • Place of Presentation
      山形大学(山形)
    • Year and Date
      2009-09-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] イネ卵細胞におけるミトコンドリアDNA量およびミトコンドリア形態2009

    • Author(s)
      高梨秀樹, 大西孝幸, 茂木美来, 岡本龍史, 有村慎一, 堤伸浩
    • Organizer
      育種学会
    • Place of Presentation
      つくば
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 被子植物における配偶子融合の分子認証機構2009

    • Author(s)
      岡本龍史
    • Organizer
      シンポジウム「動植物におけるアロ認証機構」第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Related Report
      2010 Final Research Report
  • [Book] 受精の生理(「種子のバイオサイエンス(改訂版)」)(原田久也編)2009

    • Author(s)
      岡本龍史
    • Publisher
      学会出版センター
    • Related Report
      2010 Final Research Report
  • [Book] 種子の科学とバイオテクノロジー(執筆分担、受精の生理)2008

    • Author(s)
      岡本龍史
    • Total Pages
      3
    • Publisher
      学会出版センター
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://www.biol.se.tmu.ac.jp/labo.asp?ID=horcel

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.biol.se.tmu.ac.jp/labo.asp?ID=horcel

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://dept.biol.metro-u.ac.jp/labo.asp?lD=horcel

    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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