Integrated analysis of the foregut formation and regionalization of the endoderm

• Project/Area Number: 20570207
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: FUKUDA Kimiko Tokyo Metropolitan University, 大学院・理工学研究科, 准教授 (40285094)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 器官形成 / 消化管 / 内胚葉 / シグナル分子 / 消化器官 / 境界形成 / 転写因子 / 相互作用 / 領域化 / マイクロアレイ / 細胞運動 / 細胞標識 / 肝臓 / 脂質代謝

Research Abstract

Much about molecular mechanisms involved in regionalization of the gut endoderm remain unknown. To clarify this we isolated genes expressed in the early endoderm locally, and analyzed their function. We found that sizzled modified gradient of BMP ligand and regulated liver and stomach differentiation. Also, APOA1 induced liver endoderm at the pancreas region. In addition, the mechanism of boundary formation between the stomach and the intestine were controlled by two transcriptional factors, which are expressed in each region.

Research Products

• [Journal Article] Identification of region specific genes in early chicken endoderm. (2011)
  • Author(s): Kimura, W., Cantas, A. Sheng, G. Jakt, M., Yasugi, S., Fukuda, K.
  • Journal Title: Gene Exp.Patterns in press
• [Journal Article] Conserved origin of the ventral pancreas in chicken. (2009)
  • Author(s): Matsuura, K., Katsumoto, K. Fukuda, K., Kume, K., Kume, S.
  • Journal Title: Mech.Dev. 126 Pages: 817-827
• [Journal Article] Origin of pancreatic precursors in the chick embryo and the mechanism of endoderm regionalization. (2009)
  • Author(s): Katsumoto, K., Fukuda, K., Kimura, W., Shimamura, K., Yasugi, S., Kume, S.
  • Journal Title: Mech.Dev. 126 Pages: 539-551
• [Journal Article] Origin of pancreatic precursors in the chick embryo and the mechanism of endoderm regionalization. (2009)
  • Author(s): Katsumoto, K., Fukuda, K., Kimura, W., Shimamura, K., Yasugi, S., Kume, S.
  • Journal Title: Mechanism of Development 126 Pages: 539-551
  • Peer Reviewed
• [Journal Article] Conserved origin of the ventral pancreas in chicken. (2009)
  • Author(s): Matsuura, K., Katsumoto, K., Fukuda, K., Kume, K., Kume, S.
  • Journal Title: Mechanism of Development 126 Pages: 817-827
  • Peer Reviewed
• [Journal Article] Origin of pancreatic precursors in the chick embryo and the mechanism of endoderm regionalization. (2009)
  • Author(s): K. Katsumoto, K. Fukuda, W. Kimura, K. Shimamura, S. Yasugi, S. Kume
  • Journal Title: Mechanisms of Development (印刷中)
  • Peer Reviewed
• [Journal Article] Identification of region specific genes in early chicken endoderm.
  • Author(s): Kimura, W., Cantas, A.Sheng G.Jakt, M., Yasugi, S., Fukuda, K.
  • Journal Title: Gene Expression Patterns Volume: 印刷中
  • Peer Reviewed
• [Presentation] The molecular mechanisms involved in the regionalization of the gut. (2011)
  • Author(s): Kimiko Fukuda
  • Organizer: UOS-TMU Joint Workshop
  • Place of Presentation: KANGCHON TRAINING CENTER,韓国
  • Year and Date: 2011-02-11
• [Presentation] The functinola analysis of Apolipoprotain A1 in Xenopus Laevis. (2011)
  • Author(s): Haruka Someya, Kimiko Fukuda
  • Organizer: UOS-TMU Joint Workshop
  • Place of Presentation: KANGCHON TRAINING CENTER,韓国
  • Year and Date: 2011-02-10
• [Presentation] The mechanisms of the boundary formation between the stomach and intestine endoderm in chicken embryo. (2010)
  • Author(s): Kenta Watanabe, Kimiko Fukuda
  • Organizer: 首都大バイオコンファレンス
  • Place of Presentation: 東京
  • Year and Date: 2010-11-05
• [Presentation] 前腸で発現するsizzledは肝臓領域を前腸門付近に制限する (2010)
  • Author(s): 岡山唯、木村航、八杉貞雄、福田公子
  • Organizer: 発生生物学会夏季シンポジウム2010
  • Place of Presentation: 東京
  • Year and Date: 2010-08-25
• [Presentation] The mechanisms of the boundary formation between the stomach and intestine endoderm in chicken embryo (2010)
  • Author(s): Kenta Watanabe, Kimiko Fukuda
  • Organizer: Society for Developmental Biology 69th Annual Meeting jointly with the Japanese Society of Developmental Biologists
  • Place of Presentation: アルバカーキー,アメリカ合衆国
  • Year and Date: 2010-08-07
• [Presentation] ニワトリ胚消化管内胚葉の領域化に関わる分子機構 (2010)
  • Author(s): 福田公子
  • Organizer: 第4回XCIJ首都圏支部会(XCIJ-MA)研究集会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-24
• [Presentation] Xenopus laevisにおけるApolipoproteinAlの機能解析 (2010)
  • Author(s): 染谷春香、福田公子
  • Organizer: 第4回XCIJ首都圏支部会(XCIJ-MA)研究集会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-24
• [Presentation] The mechanisms of the boundary formation between the stomach and intestine endoderm in chicken embryo. (2010)
  • Author(s): 渡辺健太, 福田公子
  • Organizer: Society for Developmental Biology 69th Annual Meeting jointly with the Japanese Society of Developmental Biologists
  • Place of Presentation: Albuquerque
• [Presentation] Sizzled in the foregut restricts liver region into anterior intestinal portal in the chicken embryo. (2010)
  • Author(s): Okayama, Y, Kimura W., Yasugi S., Fukuda K.
  • Organizer: 43^<rd> Annual Meeting for the Japanese Society for Developmental Biologists jointly sponsored by the Asia-Pacific Developmental Biology Network
  • Place of Presentation: Kyoto
• [Presentation] Sizzled in the foregut restricts liver region into anterior intestinal portal in the chicken embryo. (2010)
  • Author(s): Yui Okayama, Wataru Kimura Sadao Yasugi, Kimiko Fukuda
  • Organizer: 43rd Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: 京都
• [Presentation] ニワトリ胚における背側、腹側膵臓形成メカニズムの解析 (2009)
  • Author(s): 勝本恵一, 松浦公美, 福田公子, 粂昭苑
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] ニワトリ胚内胚葉において胃/腸の境界形成に関わるSox2とCdxAの相互抑制作用 (2009)
  • Author(s): 渡辺健太, 福田公子
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] Analysis of origin of the pancreatic precursors and the mechanism of endoderm regionalization in the chick embryos. (2009)
  • Author(s): Keiichi Katsumoto, Kimiko Fukuda, Wataru Kimura, Kumi Matsuura, Kenji Shimamura, Sadao Yasugi, Kume Shoen
  • Organizer: 42^<nd> Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: 新潟
  • Year and Date: 2009-05-30
• [Presentation] ニワトリ胚内胚葉において胃/腸の境界形成に関わるSox2とCdxAの相互抑制作用 Repressive interaction between Sox2 and CdxA is involved in the establishment of stomach/intestine boundary in chicken embryonic endoderm. (2009)
  • Author(s): 渡辺健太, 福田公子
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] ニワトリ胚における消化管上皮分化に関わる遺伝子の探索. (2008)
  • Author(s): 武政智恵, 木村航, 八杉貞雄, 福田公子
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] Laboratory of Developmental Programs. (2008)
  • Author(s): K. Fukuda, H. Saiga
  • Organizer: 首都大バイオコンファレンス2008
  • Place of Presentation: 東京
  • Year and Date: 2008-10-23
• [Presentation] Search of genes involved in the gastrointestinal epithelial differentiation of chicken embryo. (2008)
  • Author(s): T. Takemasa, W. Kimura, S. Yasugi, K. Fukuda
  • Organizer: 首都大バイオコンファレンス2008
  • Place of Presentation: 東京
  • Year and Date: 2008-10-23
• [Presentation] Analyses of the mechanism of dorsal pancreas specification in the early chick endoderm. (2008)
  • Author(s): K. Katsumoto, K. Fukuda, W. Kimura, K. Shimamura, S. Yasugi, S. Kume
  • Organizer: 41^<st> Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: 徳島
  • Year and Date: 2008-05-29
• [Presentation] ニワトリ胚における消化管上皮分化に関わる遺伝子の探索 (2008)
  • Author(s): 武政智恵, 木村航, 八杉貞雄, 福田公子
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
• [Book] Electroporation of nucleic Acids into chick endoderm both in vitro and in ovo.(Electroporation and sonoporation in developmental biology)(H. Nakamura ed.) (2009)
  • Author(s): Kimiko Fukuda
  • Publisher: Springer
• [Book] Electroporation of nucleic Acids into chick endoderm both in vitro and in ovo. Electroporation and sonoporation in developmental biology (H. Nakamura ed. ) (2009)
  • Author(s): Kimiko Fukuda
  • Total Pages: 11
  • Publisher: Springer