Trial for production of transgenic planarians using transposon systems.

• Project/Area Number: 20570209
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: University of Hyogo
• Principal Investigator: ORI Hidefumi University of Hyogo, 大学院・生命理学研究科, 助教 (70211836)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 発生・分化 / 再生 / 遺伝子 / トランスポゾン

Research Abstract

The planarians have high regeneration activity which is attractive for developmental biologists. In order to analyze the mechanisms of the planarian regeneration at a gene level, I tried to develop a method for production of transgenic planarians using various transposon systems. I have failed to introduce foreign genes into planarians in spite of many trials with various vectors and experimental conditions.

Research Products

• [Journal Article] Planarian regeneration in the absence of blastema. (2010)
  • Author(s): Hidefumi Orii
  • Journal Title: Belgian Journal of Zoology 140巻 Pages: 145-148
  • Peer Reviewed
• [Journal Article] Planarian regeneration in the absence of blastema. (2010)
  • Author(s): Hidefumi Orii
  • Journal Title: Belgian Journal of Zoology Volume: 140 Pages: 145-148
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 小泉修 編
  • Journal Title: 動物の多様な生き方5 さまざまな神経系をもつ動物たち(共立出版) Pages: 42-55
• [Journal Article] (2009)
  • Author(s): 小泉修 編
  • Journal Title: 動物の多様な生き方5 さまざまな神経系をもつ動物たち(共立出版) Pages: 42-55
• [Presentation] プラナリアvasa様タンパク質DjVLG-Aの発現 (2010)
  • Author(s): 織井秀文, 他1名
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京大学教養学部
  • Year and Date: 2010-09-23
• [Presentation] プラナリアvasa様タンパク質DjVLG-Aの発現 (2010)
  • Author(s): 織井秀文
  • Organizer: 日本動物学会
  • Place of Presentation: 東京大学教養学部
  • Year and Date: 2010-09-23
• [Presentation] Timing of body patterning in regeneration of the planarian Dugesia japonica (2009)
  • Author(s): 織井秀文
  • Organizer: XIth International Symposium on Flatworm Biology
  • Place of Presentation: Hasselt University, Belgium
• [Presentation] Proliferating cells in the intestines of polyclads (2009)
  • Author(s): D. Okano, M. Sato, T. Tsugawa, H.Orii, S. Ishida
  • Organizer: XIth International Symposium on Flatworm Biology
  • Place of Presentation: Hasselt University, Belgium
• [Presentation] Timing of body patterning in regeneration of the planarian Dugesia japonica (2009)
  • Author(s): 織井秀文
  • Organizer: 11^<th> International Symposium on Flatworm Biology
  • Place of Presentation: Hasselt University, Belgium
• [Presentation] プラナリア再生におけるパターン形成の決定時期 (2008)
  • Author(s): 織井秀文, 他3名
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡大
  • Year and Date: 2008-09-23
• [Book] 動物の多様な生き方5さまざまな神経系をもつ動物たち (2009)
  • Author(s): 小泉修編
  • Publisher: 共立出版