Coupled role of Nodal and Notch in the establishment of mesodermal tissue

• Project/Area Number: 20570212
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Rikkyo University
• Principal Investigator: KINOSHITA Tsutomu Rikkyo University, 理学部, 教授 (30161532)
• Co-Investigator(Kenkyū-buntansha): YAMAGUCHI Masahiro 立教大学, 理学部, 助教 (00360660)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 胚葉形成 / 原腸形成 / 体節形成 / 中胚葉形成 / Nodalシグナル / Notchシグナル / Oct25 / p27Xic1 / 中胚葉誘導 / XDeltal / Xoct25 / p27Xicl

Research Abstract

In early development of Xenopus embryo, Nodal signaling induces ring-shaped mesodermal tissue in the marginal zone of gastrula embryo. To understand the molecular mechanism determining the mesoderm area, transcriptional network was examined during the mesodermal formation. Several lines of experiments showed that Nodal signaling regulates the boundary formation and the cell phase transition from growth to differentiation in the mesodermal tissue by coupling with Notch signaling.

Research Products

• [Journal Article] The analysis of the expression of a novel gene, Xenopus polka dots, which was expressed in the embryonic and larval epidermis during early development. (2011)
  • Author(s): Yoshii S, Yamaguchi M, Oogata Y, Tazaki A, Mochii M, Suzuki S, Kinoshita T.
  • Journal Title: Zool.Sci. vol.28(in press)
  • NAID: 40019070956
  • Peer Reviewed
• [Journal Article] Antagonistic role of XESR1 and XESR5 in the mesoderm formation of Xenopus laevis embryo. (2011)
  • Author(s): Kinoshita T., Haruta Y., Sakamoto C., Imaoka S.
  • Journal Title: Int.J.Dev.Biol. 55 Pages: 25-31
  • Peer Reviewed
• [Journal Article] Antagonistic role of XESR1 and XESR5 in the mesoderm formation of Xenopus laevis embryo. (2011)
  • Author(s): Kinoshita T, Haruta Y, Sakamoto C, Imaoka S.
  • Journal Title: Int.J.Develop.Biol. Volume: 55 Pages: 25-31
  • Peer Reviewed
• [Journal Article] Perturbation of Notch/Suppressor of Hairless pathway disturbs migration of primordial germ cells in Xenopus embryo. (2010)
  • Author(s): Morichika K., Kataoka K., Terayama K., Tazaki A., Kinoshita T., Watanabe K., Mochii M.
  • Journal Title: Dev.Growth Diff. 52 Pages: 235-244
  • NAID: 10027563960
  • Peer Reviewed
• [Journal Article] Perturbation of Notch/Suppressor of Hairless pathway disturbs migration of primordial germ cells in Xenopus embryo. (2010)
  • Author(s): Morichika K, Kataoka K, Terayama K, Tazaki A, Kinoshita T, Watanabe K, Mochii M.
  • Journal Title: Develop.Growth Diff. Volume: 52 Pages: 235-244
  • NAID: 10027563960
  • Peer Reviewed
• [Journal Article] Perturbation of Notch/Suppressor of Hairless pathway disturbs migration of primordial germ cells in Xenopus embryo (2010)
  • Author(s): Morichika K, Kataoka K, Terayama K, Tazaki A, Kinoshita T, Watanabe K, Mochii M.
  • Journal Title: Develop.Growth Diff. 52 Pages: 235-244
  • NAID: 10027563960
  • Peer Reviewed
• [Journal Article] Bisphenol A disruptes Notch signaling by inhibiting gamma-secretase activity and causes eye dysplasia of Xenopu laevis. (2009)
  • Author(s): Baba K., Kinoshita T, Imaoka S.
  • Journal Title: Toxicol.Sci. 108(2) Pages: 344-355
  • Peer Reviewed
• [Journal Article] Bisphenol A Disrupts Notch Signaling by Inhibiting Gamma-Secretase Activity and Causes Eye Dysplasia of Xenopus laevis (2009)
  • Author(s): Baba K, Okada K, Kinoshita T, Imaoka S.
  • Journal Title: Toxicol Sci. 108 Pages: 344-355
  • Peer Reviewed
• [Journal Article] Bisphenol A Disrupts Notch Signaling by Inhibiting Gamma-Secretase Activity and Causes Eye Dysplasia of Xenopus laevis. (2009)
  • Author(s): Baba K, Okada K, Kinoshita T, Imaoka S.
  • Journal Title: Toxicol Sci. 108(2) Pages: 344-355
  • Peer Reviewed
• [Journal Article] Mutation of DNA primase causes extensive apoptosis of retinal neurons through the activation of DNA damage checkpoint and tumor suppressor p53. (2008)
  • Author(s): Yamaguchi M., Tonou-Fujimori N., Yoshimura Y., Kishi T., Okamoto H., Masai I.
  • Journal Title: Development. 137(7) Pages: 1247-57
  • Peer Reviewed
• [Journal Article] Mutation of DNA primase causes extensive apoptosis of retinal neuronS through the activation of DNA damage checkpoint and tumor suppressor p53 (2008)
  • Author(s): Yamaguchi, M., Tonou-Fujimori, N., Yoshimura, Y., Kishi, T., Okamoto, H., Masai, I.
  • Journal Title: Development 137(7) Pages: 1247-57
  • Peer Reviewed
• [Presentation] Xenopus Oct60 needs formation of primordial germ cell. (2010)
  • Author(s): K.Morichika, K.Shimada, S.Baba, H.Kubo, T.Kinoshita
  • Organizer: 33th Ann. Meeting Mol. Biol. Society Jap.
  • Place of Presentation: Kobe
  • Year and Date: 2010-12-09
• [Presentation] Defferentiation of Xp63 expressing cells in Xenopus early embryo. (2010)
  • Author(s): Y.Ogata, M.Yamaguchi, T.Kinoshita
  • Organizer: 33th Ann. Meeting Mol. Biol. Society Jap.
  • Place of Presentation: Kobe
  • Year and Date: 2010-12-09
• [Presentation] Xenopus Oct60 is required for formation of primordial germ cell (2010)
  • Author(s): K.Morichika, K.Shimada, S.Baba, H.Kubo, T.Kinoshita
  • Organizer: 33th Ann.Meeting Mol.Biol.Society.Jap.
  • Place of Presentation: Kobe
  • Year and Date: 2010-12-09
• [Presentation] Differentiation of Xp63 expressing cells in Xenopus early embryo. (2010)
  • Author(s): Y.Ogata, M.Yamaguchi, T.Kinoshita
  • Organizer: 33th Ann.Meeting Mol.Biol.Society.Jap.
  • Place of Presentation: Kobe
  • Year and Date: 2010-12-07
• [Presentation] 表皮細胞の形質発現へ及ぼすOct60の影響 (2010)
  • Author(s): 高市佳尚、森近恵祐、木下勉
  • Organizer: 第81回日本動物学会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-25
• [Presentation] シンポジウム『シュペーマンから86年ーモデル動物を用いた受精・形態形成研究の今と昔』 (2010)
  • Author(s): 木下勉、前野貢
  • Organizer: 第81回日本動物学会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-24
• [Presentation] アフリカツメガエルの初期発生におけるOct60タンパク質の分布の変化 (2010)
  • Author(s): 嶋田啓伍、森近恵祐、久保英夫、木下勉
  • Organizer: 第81回日本動物学会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-23
• [Presentation] Transcriptional regulation of Oct60 in Xenopus oocyte (2010)
  • Author(s): K.Morichika, M.Sugimoto, K.Yasuda, T.Kinoshita
  • Organizer: 13th International Xenopus Conference
  • Place of Presentation: Canada
  • Year and Date: 2010-09-14
• [Presentation] アフリカツメガエル変態期における赤血球の増殖と甲状腺ホルモンの作用 (2009)
  • Author(s): 山口雅裕、木下勉
  • Organizer: 第80回日本動物学会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-19
• [Presentation] チョウのハネにおける鱗粉細胞配列パターンの形成機構:複数の基本機構の関与 (2009)
  • Author(s): 吉田昭広, 木下勉
  • Organizer: 第80回日本動物学会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-19
• [Presentation] Gene expression and function of Oct25 in Xenopus early embryo (2009)
  • Author(s): E.Nishitani, H.Hotta, T.Kinoshita
  • Organizer: 16th International Society of Developmental Biologists Congress
  • Place of Presentation: Scotland
  • Year and Date: 2009-09-08
• [Presentation] Gene expression and function of Oct25 in Xenopus early embryo (2009)
  • Author(s): E.Nishitani, H.Hotta, T.Kinoshita
  • Organizer: 16th International Society of Developmental Biologists Congress
  • Place of Presentation: Scotland, UK
  • Year and Date: 2009-09-08
• [Presentation] Characterization of Xpod-expressing cells in the Xenopus larval epidermis (2009)
  • Author(s): S.Yoshii, M.Yamaguchi, Y.Oogata, A.Tazaki, M.Mochii, S.Suzuki, T.Kinoshita
  • Organizer: 42nd Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Niigata
  • Year and Date: 2009-05-30
• [Presentation] Characterization of Xpod-expressing cells in the Xenopus larval epidermis (2009)
  • Author(s): S.Yoshii, M.Yamaguchi, Y.Oogata, A.Tazaki, M.Mochii, S.Suzuki, T.Kinoshita
  • Organizer: Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Niigata
  • Year and Date: 2009-05-30
• [Presentation] Analysis of proliferation and differentiation of red blood cells during metamorphosis of Xenopus laevis (2009)
  • Author(s): 山口雅裕, 木下勉
  • Organizer: 42nd Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Niigata
  • Year and Date: 2009-05-29
• [Presentation] Analysis of proliferation and differentiation of red blood cells during metamorphosis of Xenopus laevis (2009)
  • Author(s): 山口雅裕, 木下勉
  • Organizer: Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Niigata
  • Year and Date: 2009-05-29
• [Presentation] 卵母細胞におけるXoct60の転写誘導機構の解析 (2009)
  • Author(s): 杉本真人、安田克輝、木下勉
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-01-29
• [Presentation] アフリカツメガエル初期胚におけるXoct60の分化抑制機構の解析 (2009)
  • Author(s): 馬場晶悟、倉橋宏尚、高橋秀治、浅島誠、木下勉
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-01-29
• [Presentation] 今岡進:Notchシグナル阻害による発生異常の解析 (2008)
  • Author(s): 馬場一信、木下勉
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] アフリカツメガエルの初期胚におけるXoct60の機能について (2008)
  • Author(s): 東久保信人、倉橋宏尚、西脇清二、木下勉
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] アフリカツメガエルの中胚葉形成におけるNotchシグナルの役割に関する研究 (2008)
  • Author(s): 春田有紀、坂本千春、今岡進、木下勉
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] 眼の発生における低酸素応答系因子の機能解明 (2008)
  • Author(s): 佐藤夢子、木下勉、今岡進
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] アフリカツメガエル幼生皮膚に点在するXpod発現細胞の解析 (2008)
  • Author(s): 吉井俊祐、山口雅裕、田崎啓、餅井真、鈴木信太郎、木下勉
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] protein disulfide isomeraseの発生過程における生理機能解析 (2008)
  • Author(s): 澤田貴子、山崎絢子、木下勉、今岡進
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] Notchシグナル阻害による発生異常の解析 (2008)
  • Author(s): 馬場一信, 木下勉, 今岡進
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] アフリカツメガエル幼生皮膚に点在するxpod発現細胞の解析 (2008)
  • Author(s): 吉井俊祐, 山口雅裕, 田崎啓, 餅井真, 鈴木信太郎, 木下勉
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] The mechanism regulating the red blood cell transition from larval to ault type during metamorphosis of Xenopus laevis (2008)
  • Author(s): 山口雅裕, 木下勉
  • Organizer: 12th International Xenopus Conference
  • Place of Presentation: Germany
  • Year and Date: 2008-09-10
• [Presentation] Role of Notch signaling in mesoderm formation of Xenopus laevis embryo (2008)
  • Author(s): Y.Haruta, C.Sakamoto, S.Imaoka, T.Kinoshita
  • Organizer: 12th International Xenopus Conference
  • Place of Presentation: Germany
  • Year and Date: 2008-09-10
• [Presentation] The mechanism regulating the red blood cell transition from larval toault type during metamorphosis of Xenopus laevis (2008)
  • Author(s): M. Yamaguchi, T. Kinoshita
  • Organizer: 12th International Xenopus Conference
  • Place of Presentation: Germany
  • Year and Date: 2008-09-10
• [Presentation] チョウのハネにおける鱗粉細胞配列パターンの形成機構 : Notchタンパクの発現 (2008)
  • Author(s): 吉田昭広, 木下勉
  • Organizer: 第79回日本動物学会
  • Place of Presentation: 福岡
  • Year and Date: 2008-09-05
• [Presentation] The mechanism regulating the red blood cell transition from larval to adult type during metamorphosis of Xenopus laevis (2008)
  • Author(s): M.Yamaguchi, T.Kinoshita
  • Organizer: Joint Meeting of Japanese Society of DB & ISDB
  • Place of Presentation: Tokushima
  • Year and Date: 2008-05-29
• [Presentation] The mechanism regulating the red blood cell transition from larval toault type during metamorphosis of Xenopus laevis (2008)
  • Author(s): M. Yamaguchi, T. Kinoshita
  • Organizer: Joint Meeting of Japanese Society of DB & ISDB
  • Place of Presentation: Tokushima
  • Year and Date: 2008-05-29
• [Remarks] ホームページ等
  • URL: http://www2.rikkyo.ac.jp/web/tkinoshita/