Functional difference between Notch ligands Dll1 and Dll3 in mouse somitogenesis

• Project/Area Number: 20570215
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: National Institute of Health Sciences
• Principal Investigator: TAKAHASHI Yu National Institute of Health Sciences, 毒性部, 室長 (60321858)
• Co-Investigator(Renkei-kenkyūsha): KITAJIMA Satoshi 国立医薬品食品衛生研究所, 毒性部, 室長 (30270622) ; YASUHIKO Yukuto 国立医薬品食品衛生研究所, 毒性部, 主任研究官 (40370944)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 体節形成 / Notchシグナル / マウス / 遺伝学 / 分子時計 / Delta

Research Abstract

We generated a Dll3-knockin mouse, which express Dll3 instead of Dll1, and analyzed effects on somitogenesis and molecular clock mechanism. Our results suggested that Dll3 is not a Notch ligand but acts as a suppressive modulator. In addition, these mice showed segmentation of vertebral body without Notch signal oscillation, implying presence of another mechanism.

Research Products

• [Journal Article] The oscillation of Notch activation, but not its boundary, is required for somite border formation and rostral-caudal patterning within a somite. (2010)
  • Author(s): Oginuma M., Takahashi Y., Kitajima S., Kiso M., Kanno J., Kimura A., Saga Y.
  • Journal Title: Development(First 2 authors equal contribution) 137 Pages: 1515-1522
  • Peer Reviewed
• [Journal Article] The oscillation of Notch activation, but not its boundary, is required for somite border formation and rostral-caudal patterning within a somite. (2010)
  • Author(s): Masayuki Oginuma, Yu Takahashi, Satoshi Kitajima, Makoto Kiso, Jun Kanno, Akatsuki Kimura, Yumiko Saga
  • Journal Title: Development Volume: 137 Pages: 1515-1522
  • Peer Reviewed
• [Journal Article] The oscillation of Notch activation, but not its boundary, is required for somite border formation rostral-caudal patterning within a somite (2010)
  • Author(s): Masayuki Oginuma, Yu Takahashi, Satoshi Kitajima, Makoto Kiso, Jun Kanno, Akatsuki Kimura, Yumiko Saga
  • Journal Title: Development 137 Pages: 1515-1522
  • Peer Reviewed
• [Journal Article] Functional importance of evolutionally conserved Tbx6 binding sites in the presomitic mesoderm-specific enhancer of Mesp2. (2008)
  • Author(s): Yasuhiko Y., Kitajima S., Takahashi Y., Oginuma M., Kagiwada H., Kanno J., Saga Y.
  • Journal Title: Development 135 Pages: 3511-3519
  • Peer Reviewed
• [Presentation] Segmentation and rostro-caudal patterning of somites is not essential for metameric pattern of vertebral body/intervertebral disc formation. (2010)
  • Author(s): 高橋雄,安彦行人,相賀裕美子,菅野純
  • Organizer: 第43回日本発生生物学会
  • Place of Presentation: 京都
• [Presentation] Segmentation and rostro-caudal patterning of somites is not essential for metameric pattern of vertebral body/intervertebral discformation. (2010)
  • Author(s): 高橋雄
  • Organizer: 第43回日本発生生物学会
  • Place of Presentation: 京都国際会館
• [Presentation] Segmentation and rostro-caudal patterning of somites is not essential for vertebral body segmentation. (2009)
  • Author(s): 高橋雄,安彦行人,相賀裕美子,菅野純
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Segmentation and rostro-caudal patterning of somites is not essential for vertebral body segmentation (2009)
  • Author(s): 高橋雄
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Novel mouse Delta-like 1 (Dll1) alleles yield insights into involvement of Notch signal in segmentation of vertebrae. (2008)
  • Author(s): 高橋雄,北嶋聡,安彦行人,菅野純,相賀裕美子
  • Organizer: BMB2008
  • Place of Presentation: 神戸
• [Presentation] Overexpression of Delta-like 3 (Dll3) in the presomitic mesoderm causes defects in the axial skeletal formation. (2008)
  • Author(s): 高橋雄,北嶋聡,安彦行人,菅野純,相賀裕美子
  • Organizer: 第3回Notch研究会
  • Place of Presentation: 三島
• [Presentation] Delta-like 3 (Dll3) does not substitute for Delta-like 1 (Dll1) in somitogenesis in vivo but modulates Dll1/Notch signaling in the posterior PSM. (2008)
  • Author(s): 高橋雄,北嶋聡,安彦行人,菅野純,相賀裕美子
  • Organizer: 第41回日本発生生物学会
  • Place of Presentation: 徳島
• [Presentation] Delta-like 3 (Dll3) does not substitute for Delta-like 1 (Dll1) in somit ogenesis in vivo but modulates Dll1/Notch signaling in the posterior PSM (2008)
  • Author(s): 高橋雄
  • Organizer: 第41回日本発生生物学会
  • Place of Presentation: 徳島県郷土文化会館
• [Presentation] Novel mouse Delta-like 1 (Dll1) alleles yield insights into involvement of Notch signal in segmentation of vertebrae (2008)
  • Author(s): 高橋雄
  • Organizer: BMB2008
  • Place of Presentation: 神戸ポートアイランド
• [Book] 生物学辞典(石川統他編) (2010)
  • Author(s): 高橋雄, 他
  • Total Pages: 1600
  • Publisher: 東京化学同人,東京
• [Book] 4章2-II)ホールマウントin situハイブリダイゼーション,改訂第5版新遺伝子工学ハンドブック(村松正実,山本雅,岡崎康司編) (2010)
  • Author(s): 高橋雄
  • Publisher: 羊土社,東京
• [Book] 生物学辞典 (2010)
  • Author(s): 石川統, 他編
  • Total Pages: 1600
  • Publisher: 東京化学同人
• [Book] 改訂第5版 新遺伝子工学ハンドブック (2010)
  • Author(s): 村松正実、山本雅、岡崎康司編
  • Total Pages: 360
  • Publisher: 羊土社