| Project/Area Number |
20570221
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
守口 和基 広島大学, 大学院・理学研究科, 講師 (30294523)
田中 克幸 広島大学, 大学院・理学研究科, 助教 (10363045)
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| Co-Investigator(Renkei-kenkyūsha) |
MORIGUCHI Kazuki 広島大学, 大学院・理学研究科, 講師 (30294523)
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| Research Collaborator |
TANAKA Katsuyuki 株式会社建設環境研究所 (10363045)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 水平遺伝子伝達 / 生物界間接合 / T-DNA輸送 / 細菌 / アグロバクテリア / 酵母 / 真核生物 / 接合遺伝子 / vir遺伝子 |
|---|
| Research Abstract |
Agrobacterial T-DNA transfer and Trans-kingdom conjugation from Gram-negative bacteria to yeasts are interesting phenomena in that bacteria actively transfer DNA horizontally to eukaryotes. This study with a yeast as a model eukaryotic recipient revealed that yeast genes mainly for vATPase subunits are important for Trans-kingdom conjugation, and that yeast genes including RAD2 and SRS2 are important for the agrobacteria-mediated transformation. Each of the genes is likely to function in a process which had been specialized between the two phenomena. This study also developed useful tools for accurate but easy modification on huge-size plasmids and enabled highly efficient but easy transformation.
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