| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Research Abstract |
Non-homologous end-joining (NHEJ) is a major repair pathway of DNA double-strand breaks (DSBs) in human cells. Ku and XLF coordinately function in the recognition of DSBs in NHEJ. In this study, we showed that inositol-6-phosphate enhances the recognition of DSBs by XLF in the presence of Ku. Furthermore, we showed a critical role of Ku-XLF interaction in the molecular assembly of NHEJ factors on DSBs and biological significance of a genetic mutation found in patients of human genetic disorder. Based on these results, we proposed a new model for the DSB recognition in NHEJ.
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