Analysis of a novel signaling pathway that regulates DNA damage recognition mediated by XLF
| Project/Area Number |
20579003
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Kumamoto University |
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Principal Investigator |
YANO Ken-ichi Kumamoto University, バイオエレクトリクス研究センター, 教授 (70311230)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | DNA損傷 / DNA二重鎖切断 / DNA修復 / 非相同末端連結 / XLF / Ku / XRCC4 / イノシトール6リン酸 / 細胞内情報伝達 |
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| Research Abstract |
Non-homologous end-joining (NHEJ) is a major repair pathway of DNA double-strand breaks (DSBs) in human cells. Ku and XLF coordinately function in the recognition of DSBs in NHEJ. In this study, we showed that inositol-6-phosphate enhances the recognition of DSBs by XLF in the presence of Ku. Furthermore, we showed a critical role of Ku-XLF interaction in the molecular assembly of NHEJ factors on DSBs and biological significance of a genetic mutation found in patients of human genetic disorder. Based on these results, we proposed a new model for the DSB recognition in NHEJ.
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Report
(4 results)
Research Products
(38 results)
