Structure analysis of S. cerevisiae Rap1-telomeric DNA complex by electron microscopy
| Project/Area Number |
20579004
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
MAKI Saori 独立行政法人理化学研究所, タンパク質結晶構造解析研究グループ, 研究員 (20513386)
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| Co-Investigator(Renkei-kenkyūsha) |
YONEKURA Koji 独立行政法人理化学研究所, 米倉生体機構研究室, 准主任研究員 (50346144)
SAKO Yasushi 独立行政法人理化学研究所, 佐甲細胞情報研究室, 主任研究員 (20215700)
HIROSHIMA Michio 独立行政法人理化学研究所, 佐甲細胞情報研究室, 研究員
ELIZABETH H. Blackburn University of California, San Francisco Department of Biochemistry and Biophysics, 教授
TANYA L. Williams University of California, San Francisco Department of Biochemistry and Biophysics, 研究員
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| Project Period (FY) |
2008 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,050,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | テロメア / タンパク質複合体 / 出芽酵母 / 電子顕微鏡 / 電子顕微鏡法 / 核酸 / 蛋白質 / 老化 / ガン |
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| Research Abstract |
Telomeres are specialized nucleoprotein complexes that cap the ends of eukaryotic linear chromosomes, stabilizing them and protecting their integrity. In budding yeast major telomere binding protein Rap1 controls telomere length. We prepared Rap1 and GFP fusion Rap1, and analyzed their structures by electron microscopy. Electron tomography and high resolution fluorescence microscopy revealed three-dimensional structures of unique filaments made of Rap1.
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Report
(7 results)
Research Products
(10 results)
