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Structure analysis of S. cerevisiae Rap1-telomeric DNA complex by electron microscopy

Research Project

Project/Area Number 20579004
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

MAKI Saori  独立行政法人理化学研究所, タンパク質結晶構造解析研究グループ, 研究員 (20513386)

Co-Investigator(Renkei-kenkyūsha) YONEKURA Koji  独立行政法人理化学研究所, 米倉生体機構研究室, 准主任研究員 (50346144)
SAKO Yasushi  独立行政法人理化学研究所, 佐甲細胞情報研究室, 主任研究員 (20215700)
HIROSHIMA Michio  独立行政法人理化学研究所, 佐甲細胞情報研究室, 研究員
ELIZABETH H. Blackburn  University of California, San Francisco Department of Biochemistry and Biophysics, 教授
TANYA L. Williams  University of California, San Francisco Department of Biochemistry and Biophysics, 研究員
Project Period (FY) 2008 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,050,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥800,000 (Direct Cost: ¥800,000)
Keywordsテロメア / タンパク質複合体 / 出芽酵母 / 電子顕微鏡 / 電子顕微鏡法 / 核酸 / 蛋白質 / 老化 / ガン
Research Abstract

Telomeres are specialized nucleoprotein complexes that cap the ends of eukaryotic linear chromosomes, stabilizing them and protecting their integrity. In budding yeast major telomere binding protein Rap1 controls telomere length. We prepared Rap1 and GFP fusion Rap1, and analyzed their structures by electron microscopy. Electron tomography and high resolution fluorescence microscopy revealed three-dimensional structures of unique filaments made of Rap1.

Report

(7 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report   Self-evaluation Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (10 results)

All 2013 2010 2008

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (8 results)

  • [Journal Article] Characterization of the Yeast Telomere Nucleoprotein Core : Rap1 binds independently to each recognition site2010

    • Author(s)
      Tanya L. Williams, Daniel L. Levy, Saori Maki-Yonekura, Koji Yonekura and Elizabeth H. Blackburn
    • Journal Title

      Journal of Biological Chemistry

      Volume: 285 Pages: 35814-35824

    • Related Report
      2012 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Blackburn Characterization of the yeast telomere nucleoprotein core. Rap1 binds independently to each recognition site.2010

    • Author(s)
      Tanya L.William, Daniel L.Levy, Saori Maki-Yonekura, Koji Yonekura, Elizabeth H.
    • Journal Title

      Journal of Biological Chemistry Vol.285, No.46

      Pages: 35814-35824

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Presentation] Structure analysis of S. cereisiae Rap1-telomeric DNA complex by single molecule imaging and electron microscopy2013

    • Author(s)
      Saori Maki-Yonekura, Michio Hiroshima, Tanya L. Williams, Yasushi Sako, Elizabeth H. Blackburn and Koji Yonekura
    • Organizer
      Nagoya Symposium - Frontiers in Structural Physiology
    • Place of Presentation
      名古屋大学豊田講堂(愛知県)
    • Related Report
      2012 Final Research Report
  • [Presentation] Structure analysis of S. cerevisiae Rap1-telomeric DNA complex by single molecule imaging and electron microscopy2013

    • Author(s)
      眞木さおり
    • Organizer
      Nagoya Symposium - Frontiers in Structural Physiology
    • Place of Presentation
      名古屋大学豊田講堂(愛知県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Correlative Imaging---CXDI and Cryo-EM---.2010

    • Author(s)
      Koji Yonekura, Saori Maki-Yonekura
    • Organizer
      The 3^<rd> Workshop on FEL Science : "Emerging X-ray applications in biological systems-II".
    • Place of Presentation
      Kitayuzawa, Hokkaido, Japan
    • Year and Date
      2010-10-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Cryo-electron microscopy and helical reconstruction of biological materials.2010

    • Author(s)
      眞木さおり(代表)
    • Organizer
      日本顕微鏡学会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2010-05-23
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] Cryo-electron microscopy and helical image reconstruction of biological materials.2010

    • Author(s)
      Saori Maki-Yonekura, Koji Yonekura
    • Organizer
      日本顕微鏡学会アジア若手シンポジウム
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2010-05-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] 電子顕微鏡法による出芽酵母Rap1とテロメアDNA複合体の構造解析2008

    • Author(s)
      眞木(米倉)さおり、Tanya L. Williams, Elizabeth H. Blackburn, 米倉 功治
    • Organizer
      第46回日本生物物理学会年会
    • Place of Presentation
      福岡国際センター(福岡県)
    • Year and Date
      2008-12-04
    • Related Report
      2012 Final Research Report
  • [Presentation] 電子顕微鏡法による出芽酵母Rap1とテロメアDNA複合体の構造解析2008

    • Author(s)
      米倉さおり(眞木さおり)
    • Organizer
      第46回日本生物物理学会年会
    • Place of Presentation
      福岡国際センター
    • Year and Date
      2008-12-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] 電子顕微鏡法による出芽酵母Rap1とテロメアDNA複合体の構造解析2008

    • Author(s)
      眞木さおり(代表)
    • Organizer
      日本生物物理学会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2010 Self-evaluation Report

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Published: 2008-04-01   Modified: 2019-07-29  

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