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Establishment and utilization of simple and rapid soybean transformation system in a Japanese variety, Kariyutaka

Research Project

Project/Area Number 20580003
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionHokkaido University

Principal Investigator

YAMADA Tetsuya  Hokkaido University, 大学院・農学研究院, 助教 (70374618)

Co-Investigator(Kenkyū-buntansha) KITAMURA Keisuke  北海道大学, 大学院・農学研究院, 教授 (50111240)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords形質転換 / 大豆 / アグロバクテリウム / 種子 / Myb転写因子 / フラボノイド / ダイズ / 転写因子
Research Abstract

An efficient and stable transformation technology in higher plants is used to identify the gene function or to develop new varieties. Soybean transformation has continued to be laborious and the frequency of recovered transgenic plants is still low. We found that a Japanese soybean variety, Kariyutaka, possessed advantageous characters for transformation. We developed a simple and rapid transformation system in Kariyutaka. The transformation frequency in the method presented in this study was 4 times as high as in that obtained with our previous method.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (14 results)

All 2011 2010 2009 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (9 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Cotyledonary node pre-wounding with a micro-brush increased frequency of Agrobacterium-mediated transformation in soybean.2010

    • Author(s)
      T Yamada, S Watanabe, M Arai, K Harada, K Kitamura
    • Journal Title

      Plant Biotechnology 27

      Pages: 217-220

    • NAID

      10026527390

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Soybean stem growth habit gene Dt1 is an orthologue of Arabidopsis TFL1.2010

    • Author(s)
      Liu B, S Watanabe, T Uchimiya, F Kong, A Kanazawa, Z Xia, A Nagamatsu, M Arai, T Yamada, K Kitamura, Masuta C, K Harada, J Abe
    • Journal Title

      Plant Physiology 153

      Pages: 198-210

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cotyledonary node pre-wounding with a micro-brush increased frequency of Agrobacterium-mediated transformation in soybean2010

    • Author(s)
      Tetsuya Yamada, et al.
    • Journal Title

      Plant Biotechnology (印刷中)

    • NAID

      10026527390

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 形質転換技術を利用したダイズ種子成分の代謝工学2011

    • Author(s)
      山田哲也
    • Organizer
      第5回ダイズ研究会(招待講演)
    • Place of Presentation
      北海道大学
    • Year and Date
      2011-03-05
    • Related Report
      2010 Final Research Report
  • [Presentation] 形質転換技術を利用したダイズ種子成分の代謝工学2011

    • Author(s)
      山田哲也
    • Organizer
      第5回大豆研究会
    • Place of Presentation
      北海道大学 招待講演
    • Year and Date
      2011-03-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] 形質転換ダイズを利用した種子貯蔵タンパク質11Sグロブリンサブユニット遺伝子のプロモーター解析2010

    • Author(s)
      中村賢太郎, 他
    • Organizer
      種子生理生化学研究会
    • Place of Presentation
      滋賀県・休暇村近江八幡
    • Year and Date
      2010-12-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヘキソースリン酸トランスポーター遺伝子の過剰発現ダイズにおける脂肪酸組成と脂質含量2010

    • Author(s)
      山田哲也, 他
    • Organizer
      日本植物細胞分子生物学会
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] アグロバクテリウムを介したダイズ形質転換系の確立とその利用2009

    • Author(s)
      山田哲也・松田史生・斉藤和季・新井麻衣子・渡辺啓史・原田久也・喜多村啓介
    • Organizer
      第27回日本植物細胞分子生物学会
    • Place of Presentation
      日本大学
    • Year and Date
      2009-07-31
    • Related Report
      2010 Final Research Report
  • [Presentation] アグロバクテリウムを介したダイズ形質転換系の確立とその利用2009

    • Author(s)
      山田哲也, 他
    • Organizer
      第27回日本植物細胞分子生物学会
    • Place of Presentation
      藤沢市,日本大学
    • Year and Date
      2009-07-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] 国内ダイズ品種カリユタカを用いた高効率かつ迅速な形質転換系の開発2009

    • Author(s)
      山田哲也
    • Organizer
      日本育種学会
    • Place of Presentation
      茨城県つくば市つくば国際会議場
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] Establishment of stable transformation system mediated by Agrobacterium in Japanese soybean variety.2009

    • Author(s)
      山田哲也, 新井麻衣子, 松田史生, 斉藤和季, 喜多村啓介
    • Organizer
      World Soybean Conference Research VIII P.232
    • Place of Presentation
      中国北京
    • Related Report
      2010 Final Research Report
  • [Presentation] Establishment of stable transformation system mediated by Agrobacterium in Japanese soybean variety.2009

    • Author(s)
      Tetsuya Yamada, et al.
    • Organizer
      World Soybean Conference Research VIII
    • Place of Presentation
      北京,中国
    • Related Report
      2009 Annual Research Report
  • [Book] ダイズのゲノム科学と組換え育種の現状と展望、サイエンスフォーラム大豆のすべて(山田哲也担当)2010

    • Author(s)
      喜多村啓介編集
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.agr.hokudai.ac.jp/ikushu/idenshigen/

    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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