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Integration of chromosome maps of allogamous diploid red clover.

Research Project

Project/Area Number 20580006
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionKobe University

Principal Investigator

OHMIDO Nobuko  Kobe University, 人間発達環境学研究科, 教授 (30343263)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsマメ科植物 / 染色体地図 / 遺伝子地図 / FISH / 画像解析 / アカクローバ / ゲノム解析
Research Abstract

Red clover (Trifolium pratense L.) is a forage legume, an allogamous diploid (2n=2x=14) with 440Mb genome size. Seven prometaphase chromosomes were identified by fluorescence in situ hybridization (FISH) using 26S and 5S rDNA probes and bacterial artificial chromosome (BAC) clones and chromosome image analysis system ver.4.0 (CHIAS4). 14 BAC clones located close to the both end of each linkage group (LG), representatively LG1-7, exclusively hybridized to the distal regions of Chromosome 4, 2, 6, 5, 1, 7 and 3, respectively. Chromosomes images of FISH mapping were quantitatively analyzed based on CHIAS4 and construct of quantitative idiogram. This is the first complete chromosome map of red clover and integration of the genetic linkage maps and the quantitative chromosome maps provides valuable information on the genetic data and insight into the legumes genetics. This study should contribute to provide significant data for DNA markers and molecular breeding in legume varieties.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (23 results)

All 2010 2009 2008 Other

All Journal Article (11 results) (of which Peer Reviewed: 5 results) Presentation (11 results) Remarks (1 results)

  • [Journal Article] Recent advances in rice genome and chromosome structure research by fluorescence in situ hybridization (FISH) The Proceedings of the Japan Academy2010

    • Author(s)
      N.Ohmido, K.Fukui, T.Kinoshita
    • Journal Title

      Series B12. 86

      Pages: 103-116

    • Related Report
      2010 Final Research Report
  • [Journal Article] Physical mapping of ribosomal DNA genes on Jatropha curcas chromosomes by multicolor FISH.2010

    • Author(s)
      M.Witkowska, N.Ohmido, J.Cartagena, N.Shibagaki, S.Kajiyama, K.Fukui
    • Journal Title

      Cytologia 74

      Pages: 133-139

    • NAID

      10025405446

    • Related Report
      2010 Final Research Report
  • [Journal Article] Integration of cytogenetic and genetic linkage maps of Lotus japonicus, a model plant for legumes.2010

    • Author(s)
      N.Ohmido, A.Ishimaru, S.Kato, S.Sato, S.Tabata, K.Fukui
    • Journal Title

      Chromosome Res 18

      Pages: 287-299

    • Related Report
      2010 Final Research Report
  • [Journal Article] Image analysis of small plant chromosomes by using an improved system, CHIAS IV2010

    • Author(s)
      S.Kato, N.Ohmido, M.Hara, R.Kataoka, K.Fukui
    • Journal Title

      Chromosome Sci.

      Volume: 12 Pages: 43-50

    • NAID

      10026671047

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sequence Analysis of the Genome of an Oil-Bearing Tree, Jatropha curcas L.2010

    • Author(s)
      Sato S, Hirakawa H, Isobe S, Fukai E, Watanabe A, Kato M, Kawashima K, Minami C, Muraki A, Nakazaki N, Takahashi C, Nakayama S, Kishida Y, Kohara M, Yamada M, Tsuruoka H, Sasamoto S, Tabata S, Aizu T, Toyoda A, Shin-I T, Minakuchi Y, Kohara Y, Fujiyama A, Tsuchimoto S, Kajiyama S, Makigano E, Ohmido N, Shibagaki N, Cartagena JA, Wada N, Kohinata T, Atefeh A, Yuasa S, Matsunaga S, Fukui K.
    • Journal Title

      DNA Res.

      Pages: 1-12

    • NAID

      120006994998

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Integration of cytogenetic and genetic linkage maps of Lotus japonicus, a model plant for legumes2010

    • Author(s)
      Nobuko Ohmido, Akiko Ishimaru, Seiji Kato, Shusei Sato, Satoshi Tabata, Kiichi Fukui
    • Journal Title

      Chromosome Research 18(未定(現時点オンライン版))

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recent advances in rice genome and chromosome structure research by fluorescence in situ hybridization(FISH)2010

    • Author(s)
      Nobuko Ohmido, Kiichi Fukui, Toshiro Kinoshita
    • Journal Title

      Proc.Jpn.Acad.Ser.B Phys.Biol.Sci 86

      Pages: 101-116

    • NAID

      130000168767

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anti-peptide antibodies for examining the conformation, molecular assembly, and localization of an intracellular protein, ribosormal protein S6, in vivo.2008

    • Author(s)
      M.Nakagawa, N.Ohmido, K.Ishikawa, S.Uchiyama, K.Fukui, T.Azuma
    • Journal Title

      J.Biochem. 143

      Pages: 325-332

    • Related Report
      2010 Final Research Report
  • [Journal Article] Genome structure of the legume, Lotus japonicus.2008

    • Author(s)
      S.Sato, Y.Nakamura, T.Kaneko, E.Asamizu, T.Kato, M.Nakao, S.Sasamoto, A.Watanabe, A.Ono, K.Kawashima, T.Fujishiro, M.Katoh, M.Kohara, Y.Kishida, C.Minami, S.Nakayama, N.Nakazaki, Y.Shimizu, S.Shinpo, C.Takahashi, T.Wada, M.Yamada, N.Ohmido, M.Hayashi, K.F
    • Journal Title

      DNA Res. 15

      Pages: 227-239

    • NAID

      120006995002

    • Related Report
      2010 Final Research Report
  • [Journal Article] Nuclear organization analyzed by visualization.2008

    • Author(s)
      Ohmido N, Wako T, Fukui K.
    • Journal Title

      The Nucleus 50

      Pages: 473-489

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Genome Structure of the Legume、Lotus japonicus.2008

    • Author(s)
      Sato S, Nakamura Y, KanekoT, Asamizu E, Kato T, Nakao M, Sasamoto S, Watanabe A, Ono A, Kawashima K, Fujishiro T, Katoh M, Kohara M, Kishida Y, Minami C, Nakayama S, Nakazaki N, Shimizu Y, Shinpo S, Takahashi C, Wada T, Yamada M, Ohmido N, HayasM M, Fukui K, Baba T, Nakamichi T, Mori H, Tabata S.
    • Journal Title

      DNA Research 15

      Pages: 227-239

    • NAID

      120006995002

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 新規油糧植物ジャトロファの組織培養と染色体構造の解析2010

    • Author(s)
      西川岳志・牧ヶ野衣里・姫野真由美・Cartagena Joyce・Witkowska Magdalena・土本卓・福井希一・近江戸伸子
    • Organizer
      日本育種学会第117回講演会
    • Place of Presentation
      京都市
    • Year and Date
      2010-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] ヨシの倍数性と栄養成長に関する変異について2010

    • Author(s)
      荒木哲朗・近江戸伸子・清水顕史・長谷川博
    • Organizer
      日本育種学会第117回講演会
    • Place of Presentation
      京都市
    • Year and Date
      2010-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] Characterization of Dactylis subspecies by using some classical and molecular cytogenetic methods.2010

    • Author(s)
      M.Tuna, A.Buyukbasar, E.E.Teykin, H.Budak, N.Ohmido, T.Yamada
    • Organizer
      Plant and Animal Genome XVIII
    • Place of Presentation
      San Diego
    • Year and Date
      2010-01-15
    • Related Report
      2010 Final Research Report
  • [Presentation] マメ科植物のシンテニーを利用したダイズ染色体の解析2009

    • Author(s)
      近江戸伸子・佐藤修正・原田久也
    • Organizer
      第60回染色体学会
    • Place of Presentation
      松江市
    • Year and Date
      2009-11-14
    • Related Report
      2010 Final Research Report
  • [Presentation] マメ科植物のシンテニーを利用したダイズ染色体の解析2009

    • Author(s)
      近江戸伸子・佐藤修正・原田久也
    • Organizer
      染色体学会2009年度年会
    • Place of Presentation
      くにびモチッセ(島根県)
    • Year and Date
      2009-11-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] High-resolution FISH for gene mapping and molecular analysis of plant chromosomes2008

    • Author(s)
      Ohmido N.
    • Organizer
      Asian Chromosome colloquium 3
    • Place of Presentation
      大阪大学
    • Year and Date
      2008-12-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] Comprehensive chromosome map of red clover (Trifolium pratense L. ).2008

    • Author(s)
      Kataoka R, Hara M, Kato S, Isobe S, Sato S, Tabata S, Ohmido N.
    • Organizer
      Asian Chromosome colloquium 3
    • Place of Presentation
      大阪大学
    • Year and Date
      2008-12-02
    • Related Report
      2008 Annual Research Report
  • [Presentation] マメ科3植物の染色体とゲノム研究2008

    • Author(s)
      近江戸伸子
    • Organizer
      日本育種学会
    • Place of Presentation
      滋賀県立大学
    • Year and Date
      2008-10-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] マメ科3植物の染色体とゲノム研究2008

    • Author(s)
      近江戸伸子
    • Organizer
      日本育種学会第114回講演会シンポジュウム
    • Place of Presentation
      彦根市
    • Related Report
      2010 Final Research Report
  • [Presentation] Comprehensive chromosome map of red clover (Trifolium pratense L.)2008

    • Author(s)
      R.Kataoka, M.Hara, S.Kato, S.Isobe, S.Sato, S.Tabata, N.Ohmido
    • Organizer
      The 3rd Asian Chromosome Colloquium
    • Place of Presentation
      Osaka
    • Related Report
      2010 Final Research Report
  • [Presentation] High-resolution FISH for gene mapping and molecular analysis of plant chromosomes.2008

    • Organizer
      The 3rd Asian Chromosome Colloquium
    • Place of Presentation
      Osaka
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ

    • URL

      http://www2.kobe-u.ac.jp/~ohmido/Ohmido%20Lab/

    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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