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Analysis and application of salt tolerance mechanism in B. gymnorhiza

Research Project

Project/Area Number 20580008
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionTokyo University of Technology

Principal Investigator

TADA Yuichi  Tokyo University of Technology, 応用生物学部, 教授 (80409789)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords耐塩性 / マングローブ / マングローブ(オヒルギ) / 環境 / ストレス / 遺伝子組換え / シロイヌナズナ / プロテオーム / トランスクリプトーム
Research Abstract

To identify key genes in the regulation of salt tolerance in B. gymnorhiza, we performed three kinds of omics experiments. Those are the screening of salt responsive genes, followed by their functional analysis, the comprehensive functional screening of the Agrobacterium libraries expressing the mangrove cDNAs, and the identification of salt responsive proteins by proteome analysis of the mangrove plant. The selected genes were transferred into model plants and it was confirmed that some genes could confer salt tolerance to Arabidopsis.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (42 results)

All 2011 2010 2009 2008 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (25 results) Book (4 results) Remarks (5 results)

  • [Journal Article] Expression of Bruguiera gymnorhiza BgARP1 enhances salt tolerance in transgenic Arabidopsis plants.2011

    • Author(s)
      Miyama M, Tada Y
    • Journal Title

      Euphytica 17

      Pages: 383-392

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of Bruguiera gymnorhiza BgARP1 enhances salt tolerance in transgenic Arabidopsis plants.2011

    • Author(s)
      Miyama M, Tada Y
    • Journal Title

      Euphytica

      Volume: 177 Pages: 383-392

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic analysis of salt-responsive proteins in mangrove plant, Bruguiera gymnorhiza.2009

    • Author(s)
      Tada Y, Kashimura, T
    • Journal Title

      Plant Cell Physiol. 50

      Pages: 439-446

    • NAID

      130006994311

    • Related Report
      2010 Final Research Report 2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptome profiling of the mangrove plant Bruguiera Gymnorhiza and identification of salt tolerance genes by Agrobacterium functional screening.2009

    • Author(s)
      Yamanaka T, Miyama M, Tada Y
    • Journal Title

      Biosci.Biotechnol.Biochem. 73

      Pages: 304-310

    • NAID

      10027537155

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of salt tolerance genes from the mangrove plant Bruguiera gymnorhiza using Agrobacterium functional screening.2009

    • Author(s)
      Ezawa S, Tada Y
    • Journal Title

      Plant Sci. 176

      Pages: 272-278

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptome profiling of mangrove plant, Bruguiera gymnorhiza and identification of salt tolerance genes by Agrobacterium functional screening.2009

    • Author(s)
      T. Yamanaka, M. Miyama, Y. Tada
    • Journal Title

      Biosci. Biotechnol. Biochem. 73

      Pages: 304-310

    • NAID

      10027537155

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of salt tolerance genes from the mangrove plant Bruguiera gymnorhiza using Agrobacterium functional screening.2009

    • Author(s)
      S. Ezawa, Y. Tada
    • Journal Title

      Plant Sci. 176

      Pages: 272-278

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptional and physiological study of the response of Burma mangrove (Bruguiera gymnorhiza) to salt and osmotic stress.2008

    • Author(s)
      Miyama M, Tada Y
    • Journal Title

      Plant Mol.Biol. 68

      Pages: 119-129

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] Omics-based identification of salt-tolerance genes from the mangrove plant Bruguiera gymnorhiza.2011

    • Author(s)
      Yuichi Tada, Yuta Nagase, Chisato Endo
    • Organizer
      Plant Gene Discovery Technologies
    • Place of Presentation
      Vienna
    • Related Report
      2010 Final Research Report
  • [Presentation] Omics-based identification of salt-tolerance genes from the mangrove plant Bruguiera gymnorhiza.2011

    • Author(s)
      Yuichi Tada, Yuta Nagase, Chisa to Endo
    • Organizer
      Plant Gene Discovery Technologies
    • Place of Presentation
      Vienna(オーストリア)
    • Related Report
      2010 Annual Research Report
  • [Presentation] オヒルギのアルミニウム応答性たんぱく質類似遺伝子を導入したシロイヌナズナの耐塩性2010

    • Author(s)
      遠藤千里、多田雄一
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2010 Final Research Report
  • [Presentation] オヒルギのオスモチン様遺伝子と病害応答性タンパク質様遺伝子を導入したシロイヌナズナの耐塩性2010

    • Author(s)
      長瀬優太、多田雄一
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2010 Final Research Report
  • [Presentation] オヒルギのアルミニウム応答性たんぱく質類似遺伝子を導入したシロイヌナズナの耐塩性2010

    • Author(s)
      遠藤千里、多田雄一
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫)
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] オヒルギのオスモチン様遺伝子と病害応答性タンパク質様遺伝子を導入したシロイヌナズナの耐塩性2010

    • Author(s)
      長瀬優太、多田雄一
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫)
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Identification of salt tolerance genes from Bruguiera gymnorhiza and Sprobolus virginicus (L).2010

    • Author(s)
      Tada Y
    • Organizer
      Seminar at Rajshahi University
    • Place of Presentation
      Rajshahi、Bangradesh(Invited)
    • Year and Date
      2010-11-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] オヒルギ・ソナレシバの耐塩性遺伝子スクリーニング2010

    • Author(s)
      多田雄一、遠藤千里、長瀬優太、玉城功
    • Organizer
      日本育種学会第118回講演会
    • Place of Presentation
      秋田県立大学
    • Year and Date
      2010-09-24
    • Related Report
      2010 Final Research Report
  • [Presentation] オヒルギ・ソナレシバの耐塩性遺伝子スクリーニング2010

    • Author(s)
      多田雄一, 他3名
    • Organizer
      日本育種学会第118回講演会
    • Place of Presentation
      神戸ポートアイランド(兵庫)
    • Year and Date
      2010-09-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] Identification of salt tolerance genes from the mangrove plant Bruguiera gymnorhiza by functional analysis in transgenic Arabidopsis.2010

    • Author(s)
      Yuichi Tada, Yuta Nagase, Satsuki Sawai
    • Organizer
      21st International Conference on Arabidopsis Research
    • Place of Presentation
      Yokohama
    • Year and Date
      2010-07-06
    • Related Report
      2010 Final Research Report
  • [Presentation] Identification of salt tolerance genes from the mangrove plant Bruguiera gymnorhiza by functional analysis in transgenic Arabidopsis.2010

    • Author(s)
      Yuichi Tada, Yuta Nagase, Satsuki Sawai
    • Organizer
      21st International Conference on Arabidopsis Research
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Year and Date
      2010-06-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] オヒルギ遺伝子を導入したシロイヌナズナの耐塩性と耐重金属耐性2010

    • Author(s)
      多田雄一
    • Organizer
      日本育種学会第117回講演会
    • Place of Presentation
      京都大学
    • Year and Date
      2010-03-26
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] アグロバクテリウムを宿主とした耐塩性スクリーニングで選抜したオヒルギ遺伝子を導入したシロイヌナズナの解析2010

    • Author(s)
      澤井五月・多田雄一
    • Organizer
      日本育種学会第117回講演会
    • Place of Presentation
      京都大学
    • Year and Date
      2010-03-26
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] オヒルギ遺伝子を導入した耐塩性シロイヌナズナの解析2009

    • Author(s)
      多田雄一・深山真史
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-14
    • Related Report
      2010 Final Research Report
  • [Presentation] オヒルギ遺伝子を導入した耐塩性シロイヌナズナの解析2009

    • Author(s)
      多田雄一・深山真史
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] オヒルギ遺伝子を導入した耐塩性シロイヌナズナの解析2009

    • Author(s)
      多田雄一・深山真史
    • Organizer
      日本育種学会第116回講演会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-09-26
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 砂漠緑化と食糧増産のための耐塩性植物の開発2009

    • Author(s)
      多田雄一
    • Organizer
      国際バイオEXPO2009
    • Place of Presentation
      東京ビッグサイト
    • Year and Date
      2009-07-03
    • Related Report
      2010 Final Research Report
  • [Presentation] Identification of genes involved in salt tolerance of the mangrove plant Bruguiera gymnorhiza.2009

    • Author(s)
      Yuichi Tada, Masashi Miyama, Shota Ezawa
    • Organizer
      Plant Biology 2009
    • Place of Presentation
      Honolulu
    • Related Report
      2010 Final Research Report
  • [Presentation] オヒルギの塩応答性タンパク質のプロテオーム解析2009

    • Author(s)
      柏村高朗、多田雄一
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2010 Final Research Report
  • [Presentation] Identification of genes involved in salt tolerance of the mangrove plant Bruguiera gymnorhiza2009

    • Author(s)
      Yuichi Tada, Masashi Miyama, Shota Ezawa
    • Organizer
      Plant Biology 2009
    • Place of Presentation
      Honolulu
    • Related Report
      2009 Annual Research Report
  • [Presentation] オヒルギの塩応答性タンパク質のプロテオーム解析2009

    • Author(s)
      柏村高朗, 多田雄一
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2008 Annual Research Report
  • [Presentation] オヒルギの遺伝子を導入したシロイヌナズナの耐塩性の解析2008

    • Author(s)
      多田雄一,江澤祥太,深山真史
    • Organizer
      第31回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-12
    • Related Report
      2010 Final Research Report
  • [Presentation] オヒルギの遺伝子を導入したシロイヌナズナの耐塩性の解析2008

    • Author(s)
      多田雄一, 江澤祥太, 深山真史
    • Organizer
      第31回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] Agrobacteriumをホストとした耐塩性スクリーニングで選抜したオヒルギ遺伝子を導入したシロイヌナズナの解析2008

    • Author(s)
      江澤祥太,多田雄一
    • Organizer
      日本育種学会第114回講演会
    • Place of Presentation
      滋賀県立大学
    • Year and Date
      2008-10-12
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] オヒルギの塩応答性遺伝子を導入したシロイヌナズナの解析2008

    • Author(s)
      多田雄一,深山真史
    • Organizer
      日本育種学会第114回講演会
    • Place of Presentation
      滋賀県立大学
    • Year and Date
      2008-10-11
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Book] Plant Biotechnology and Transgenic Research & Molecular Agrobiology(Eds, Thangadurai D, Othman RY, Biradar DP)(Molecular dissection of salinity tolerance mechanisms in mangrove plants for molecular breeding of salt-tolerant plants)2011

    • Author(s)
      Tada Y
    • Publisher
      Bentham Science Publishers Ltd. IL, USA(in press)
    • Related Report
      2010 Final Research Report
  • [Book] Agricultural Research Updates(Ed, Hendricks BP)(Molecular mechanisms of salt tolerance in mangrove plants Volume 1 ISBN:978-1-61324-292-6)2011

    • Author(s)
      Tada Y
    • Publisher
      Nova Science Publishers, Inc., NY, USA
    • Related Report
      2010 Final Research Report
  • [Book] Molecular mechanisms of salt tolerance in mangrove plants. In "Mangroves : Ecology, Biology and Taxonomy"2010

    • Author(s)
      Tada Y
    • Publisher
      Nova Science Publishers, Inc.(印刷中, 未定)
    • Related Report
      2009 Annual Research Report
  • [Book] Molecular dissection of salinity tolerance mechanisms in mangrove plants for molecular breeding of salt-tolerant plants, In "Plant Biotechnology and Transgenic Research & Molecular Agrobiology".(Eds, Thangadurai D, Othman RY, Biradar DP)2010

    • Author(s)
      Tada Y
    • Publisher
      Bentham Science Publishers Ltd.(印刷中, 未定)
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report
  • [Remarks] マングローブプロジェクト

    • URL

      http://www.teu.ac.jp/tada/Mangrove%20project1.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.teu.ac.jp/tada/Mangrove%20project1.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.teu.ac.jp/tada/Mangrove%20projectl.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.teu.ac.jp/tada/Mangrove%20projectl.html

    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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