A study on a mechanism of tipburn incidence and selection of resistant cultivars in lettuce

• Project/Area Number: 20580026
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Horticulture/Landscape architecture
• Research Institution: Kobe University
• Principal Investigator: UNO Yuichi 神戸大学, 大学院・農学研究科, 准教授 (90304120)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: カルシウム / Ca-Hアンチポーター / 欠乏症 / ストレス / チップバーン / 抵抗性 / レタス / 生理障害 / 診断法 / CAX / チッブバーン

Research Abstract

Lettuce(Lactuca sativa L.) tipburn is a physiological disorder caused by calcium deficiency that decreases the crop value. In this study, an efficient method was established to evaluate lettuce resistance to tipburn in vitro. A sensitive cultivar identified by this method was validated its utility as indicator plants to monitor environmental conditions. On the other hand, to identify the responsible gene for tipburn incidence, four cDNAs corresponding to cation/H^+(CAX) exchanger were isolated from lettuce(LsCAX2, 3a, 3b, and 5). Sequence alignment revealed that both A and B domains which involved in calcium selection were conserved in two LSCAXs. The different expression of LsCAX genes by CaCl_2 between sensitive and resistant cultivars suggested the relevance of these genes to tipburn incidence. AtDREB1A was supposed to be another responsible gene. Overexpression of AtDREB1A in lettuce resulted in increasing tip-burned leaves. This result indicated that tipburn incidence is involved in stress response of lettuce.

Research Products

• [Journal Article] In vitro evaluation of tipburn resistance in lettuce (2012)
  • Author(s): Koyama, R., Sanada, M., Itoh, H., Kanechi, M., Inagaki, N. and Uno, Y.
  • Journal Title: Plant Cell, Tissue and Organ Culture Volume: 108 Pages: 221-227
  • Peer Reviewed
• [Journal Article] In vitro evaluation of tipburn resistance in lettuce (Lactuca sativa L.) (2012)
  • Author(s): Koyama, R., Sanada, M., Itoh, H., Kanechi, M., Inagaki, N., Uno, Y
  • Journal Title: Plant Cell, Tissue and Organ Culture Volume: 108 Issue: 2 Pages: 221-227
  • DOI: 10.1007/s11240-011-0033-5
  • Peer Reviewed
• [Presentation] In vitro evaluation of tipburn resistance in lettuce (Lactuca sativa L.)., ASHS annual conference oral session abstracts, 54-55 (2011)
  • Author(s): Koyama, R., Sanada, M., Okubo, H., Itoh, H., Kanechi, M., Inagaki, N., Uno, Y.
  • Organizer: Annual Conference of the American Society for Horticultural Science
  • Place of Presentation: Hilton Waikoloa Village (Waikoloa, Hawaii, USA)
  • Year and Date: 2011-09-26
• [Presentation] 指標品種の利用によるレタスのチップバーン抑制技術の開発 (2011)
  • Author(s): 大久保裕史, 小山竜平, 稲垣昇, 金地通生, 宇野雄一
  • Organizer: 園芸学会近畿支部滋賀大会
  • Place of Presentation: 大津
  • Year and Date: 2011-08-31
• [Presentation] 指標品種の利用によるレタスのチップバーン抑制技術の開発 (2011)
  • Author(s): 大久保裕史・小山竜平・稲垣昇・金地通生・宇野雄一
  • Organizer: 平成23年度園芸学会近畿支部滋賀大会
  • Place of Presentation: ピアザ淡海(滋賀県)
  • Year and Date: 2011-08-31
• [Presentation] In vitro evaluation of tipburn resistance in lettuce(Lactuca sativa L.) (2011)
  • Author(s): Koyama, R., Sanada, M., Okubo, H., Itoh, H., Kanechi, M., Inagaki, N., Uno, Y.
  • Organizer: Annual Conference of the American Society for Horticultural Science
  • Place of Presentation: Hawaii
• [Presentation] レタスのチップバーン抵抗性診断法の評価 (2010)
  • Author(s): 小山竜平, 真田光浩, 伊藤博通, 金地通生, 稲垣昇, 宇野雄一
  • Organizer: 平成22年度園芸学会近畿支部兵庫大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-31
• [Presentation] レタスのCAX(cation exchanger)相同性遺伝子の同定と発現解析 (2010)
  • Author(s): 山口裕貴, 難波亨輔, 金地通生, 稲垣昇, 宇野雄一
  • Organizer: 平成22年度園芸学会近畿支部大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-31
• [Presentation] レタスのチップバーン抵抗性診断法の評価 (2010)
  • Author(s): 小山竜平, 真田光浩, 伊藤博通, 金地通生, 稲垣昇, 宇野雄一
  • Organizer: 平成22年度園芸学会近畿支部大会 要旨集p.21
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-31
• [Presentation] レタスのCAX(cation exchanger)相同性遺伝子の同定と発現解析 (2010)
  • Author(s): 山口裕貴, 難波亨輔, 金地通生, 稲垣昇, 宇野雄一
  • Organizer: 平成22年度園芸学会近畿支部大会 要旨集p.21
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-31
• [Presentation] レタスのチップバーン抵抗性診断法の評価 (2010)
  • Author(s): 小山竜平・真田光浩・伊藤博通・金地通生・稲垣昇・宇野雄一
  • Organizer: 平成22年度園芸学会近畿支部大会
  • Place of Presentation: 神戸大学(神戸市)
  • Year and Date: 2010-08-31
• [Presentation] レタスのCAX (cation exchanger)相同性遺伝子の同定と発現解析 (2010)
  • Author(s): 山口裕貴・難波亨輔・金地通生・稲垣昇・宇野雄一
  • Organizer: 平成22年度園芸学会近畿支部大会
  • Place of Presentation: 神戸大学(神戸市)
  • Year and Date: 2010-08-31
• [Presentation] In vitroにおけるレタスのチップバーン抵抗性診断法の確立 (2008)
  • Author(s): 真田光浩・伊藤博通・小山竜平・森岡愛・木村周二・金地通生・稲垣昇・宇野雄一
  • Organizer: 平成20年度園芸学会近畿支部会
  • Place of Presentation: 京都
  • Year and Date: 2008-09-05
• [Presentation] in vitroにおけるレタスのチップバーン抵抗性診断法の確立 (2008)
  • Author(s): 真田光浩・伊藤博通・小山竜平・森岡愛・木村周二・金地通生・稲垣昇・宇野雄一
  • Organizer: 平成20年度園芸学会近畿支部会 要旨集p.11
  • Place of Presentation: 京都
  • Year and Date: 2008-09-05
• [Presentation] in vitroにおけるレタスのチップバーン抵抗性診断法の確立 (2008)
  • Author(s): 真田光浩, 伊藤博通, 小山竜平, 森岡愛, 木村周二, 金地通生, 稲垣昇, 宇野雄一
  • Organizer: 平成20年度園芸学会近畿支部会
  • Place of Presentation: 京都市
  • Year and Date: 2008-09-05