The visualization of amitosis cells and genetic analysis of somatic hybrids in Citrus species
Project/Area Number |
20580034
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Horticulture/Landscape architecture
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Research Institution | University of Miyazaki |
Principal Investigator |
KUNITAKE Hisato University of Miyazaki, 農学部, 教授 (80289628)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
Keywords | カンキツ / 細胞融合 / アミトーシス / 育種 / 染色体 / 細胞癒合 / ウンシュウミカン / 倍数体 / 懸濁培養細胞 |
Research Abstract |
Tetraploid culture cells were induced successfully from the long term-subcultured embryogenic calli of Satsuma mandarin (Citrus unshiu Marc.), although it was not possible to observe the amitosis cells. Genetic analysis of the putative triploid somatic hybrid SG1 obtained from electrofusion between Shougun mandarin (C.reticulata Blanc., 2n=2x=18)and L-1 grapefruit (C.paradisi Macf., 2n=2x=18)were investigated. Results of flow cytometry and RAPD analyses suggested that this was a hybrid with the haploid genome of the shougun mandarin. This triploid somatic hybrid SG1 was also the grapefruit with excellent characteristics such as seedless and parthenocarpy.
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Report
(4 results)
Research Products
(31 results)
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[Presentation]2008
Author(s)
安田喜一, 國武久登, 中川匠子, 黒木宏憲, 八幡昌紀, 平田力也, 吉倉幸博, 川上郁夫, 杉本安寛
Organizer
平成20年度園芸学会秋季大会
Place of Presentation
三重大学
Related Report
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[Book] 生物学辞典2010
Author(s)
國武久登, 他多数
Total Pages
1615
Publisher
株式会社 東京化学同人
Related Report
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