| Project/Area Number |
20580035
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Toshiya 独立行政法人農業食品産業技術総合研究機構, 果樹研究所, 研究チーム長 (60355360)
TAKADA Norio 独立行政法人農業食品産業技術総合研究機構, 果樹研究所, 主任研究員 (50355369)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | CMA / FISH / rDNA / 蛍光染色 / ゲノム / 染色体 / ナシ / レトロトランスポゾン / DAPI / in situハイブリダイゼーション / リボゾームRNA遺伝子 / 18S-5.8S-25S / 細胞遺伝学 / 酵素解離 / クロモマイシン / PI |
|---|
| Research Abstract |
Studies of chromosome were conducted to progress breeding of pear through genome studies. The results are as follows ;(1) Preparation of good chromosome sample using enzymatic maceration and air drying method was established, (2) The chromosome configuration was elucidated by banding technique using fluorochrome of CMA and DAPI, (3) The gene sites of rDNA and retrotransposons on chromosomes were detected by FISH(fluorescent in situ hybridization).
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