Karyotyping and visualization of chromosomal sites of useful genes in pear

• Project/Area Number: 20580035
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Horticulture/Landscape architecture
• Research Institution: Kagoshima University
• Principal Investigator: YAMAMOTO Masashi 鹿児島大学, 農学部, 准教授 (00305161)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Toshiya 独立行政法人農業食品産業技術総合研究機構, 果樹研究所, 研究チーム長 (60355360) ; TAKADA Norio 独立行政法人農業食品産業技術総合研究機構, 果樹研究所, 主任研究員 (50355369)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: CMA / FISH / rDNA / 蛍光染色 / ゲノム / 染色体 / ナシ / レトロトランスポゾン / DAPI / in situハイブリダイゼーション / リボゾームRNA遺伝子 / 18S-5.8S-25S / 細胞遺伝学 / 酵素解離 / クロモマイシン / PI

Research Abstract

Studies of chromosome were conducted to progress breeding of pear through genome studies. The results are as follows ;(1) Preparation of good chromosome sample using enzymatic maceration and air drying method was established, (2) The chromosome configuration was elucidated by banding technique using fluorochrome of CMA and DAPI, (3) The gene sites of rDNA and retrotransposons on chromosomes were detected by FISH(fluorescent in situ hybridization).

Research Products

• [Journal Article] Fluorescent staining and fluorescence in situ hybridization of rDNA of chromosomes in pear(Pyrus spp.) (2012)
  • Author(s): Yamamoto, M., Takada, N., Yamamoto, T., Terakami, S., Shigeta, N., Kubo, T. and Tominaga, S.
  • Journal Title: Journal of the Japanese Society for Horticultural Science Volume: 81(1) Pages: 35-40
  • NAID: 40019137054
  • Peer Reviewed
• [Journal Article] Fluorescent staining and fluorescence in situ hybridization of rDNA of chromosomes in pear (Pyrus spp.) (2012)
  • Author(s): Yamamoto, M., Takada, N., Yamamoto, T., Terakami, S., Shigeta, N., Kubo, T., Tominaga, S.
  • Journal Title: Journal of the Japanese Society for Horticultural Science Volume: 81 Pages: 35-40
  • NAID: 40019137054
  • Peer Reviewed
• [Journal Article] Molecular characterization of novel Ty1-copia-like retrotransposons in pear(Pyrus pyrifolia) (2011)
  • Author(s): Kim, H., Yamamoto, M., Hosaka, F., Terakami, S., Nishitani, C., Sawamura, Y., Yamane, H., Wu, J., Matsumoto, T., Matsuyama, T. and Yamamoto, T.
  • Journal Title: Tree Genetics & Genomes Volume: 7 Pages: 845-856
  • Peer Reviewed
• [Journal Article] Molecular characterization of novel Tyl-copia-like retrotransposons in pear (Pyrus pyrifolia) (2011)
  • Author(s): Kim, H., Yamamoto, M., Hosaka, F., Terakami, S., Nishitani, C., Sawamura, Y., Yamane, H., Wu, J., Matsumoto, T., Matsuyama, T., Yamamoto, T
  • Journal Title: Tree Genetics & Genomes Volume: 7 Pages: 845-856
  • Peer Reviewed
• [Journal Article] Detection of the ribosomal RNA gene in pear(Pyrus spp.) using fluorescence in situ hybridization (2010)
  • Author(s): Yamamoto, M., Terakami, S., Yamamoto, T., Takada, N., Kubo, T. and Tominaga, S.
  • Journal Title: Journal of the Japanese Society for Horticultural Science Volume: 79(4) Pages: 335-339
  • NAID: 130004951614
  • Peer Reviewed
• [Journal Article] Fluorescentstaining analysis of chromosomes in pear(Pyrus spp.) (2010)
  • Author(s): Yamamoto, M., Takada, N., Hirabayashi, T., Kubo, T. and Tominaga, S.
  • Journal Title: Journal of the Japanese Society for Horticultural Science Volume: 79(1) Pages: 23-26
  • Peer Reviewed
• [Journal Article] Fluorescent staining analysis of chromosomes in pear(Pyrus spp.) (2010)
  • Author(s): Yamamoto, M., Takada, N., Hirabayashi, T., Kubo, T., Tominaga, S.
  • Journal Title: Journal of the Japanese Society for Horticultural Science 79 Pages: 23-26
  • NAID: 40016940762
  • Peer Reviewed
• [Presentation] ナシ(Pyrus spp.)における染色体の蛍光染色およびリボゾームDNAの蛍光in situハイブリダイゼーション (2011)
  • Author(s): 山本雅史・高田教臣・山本俊哉・寺上伸吾・滋田徳美・久保達也・冨永茂人
  • Organizer: 園芸学会平成23年度春季大会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2011-03-20
• [Presentation] ニホンナシにおけるcopia型トランスポゾンの発現と系統解析 (2010)
  • Author(s): Hoytaek Kim・山本雅史・保坂ふみ子・寺上伸吾・西谷千佳子・澤村豊・松山知樹・山本俊哉
  • Organizer: 園芸学会平成22年度春季大会
  • Place of Presentation: 日本大学
  • Year and Date: 2010-03-22
• [Presentation] ナシ(Pyrus spp)における染色体の蛍光染色 (2010)
  • Author(s): 山本雅史・高田教臣・平林利郎・久保達也・冨永茂人
  • Organizer: 園芸学会平成21年度春季大会
  • Place of Presentation: 明治大学
  • Year and Date: 2010-03-20
• [Presentation] ナシ(Pyrus spp.)における染色体の蛍光染色 (2009)
  • Author(s): 山本雅史, 他4名
  • Organizer: 園芸学会平成21年度春季大会
  • Place of Presentation: 明治大学駿河台キャンパス(東京)
  • Year and Date: 2009-03-20