| Project/Area Number |
20580045
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant pathology
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
MORIYAMA Hiromitsu Tokyo University of Agriculture and Technology, 大学院・農学研究院, 講師 (20392673)
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| Co-Investigator(Renkei-kenkyūsha) |
FUKUHARA Toshiyuki 東京農工大学, 大学院・農学研究院, 教授 (90228924)
TERAOKA Tohru 東京農工大学, 大学院・農学研究院, 教授 (60163903)
ARIE Tsutomu 東京農工大学, 大学院・農学研究院, 教授 (00211706)
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| Research Collaborator |
KODAMA Motoichiro 鳥取大学, 農学部, 教授 (00183343)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 病原性因子 / マイコウイルス / 弱毒化ウイルス / 2本鎖RNAウイルス / イネいもち病菌 / アルタナリア・アルタナータ菌 / 生物防除資材 / ウイルス農薬 / 環境低負荷型農業 / 弱毒化 / アルタナリア・アルタナータ / 微生物農薬 |
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| Research Abstract |
Mycovirus causing impaired growth and abnormal pigmentation of the host was found in rice blast fungus, Magnaporthe oryzae. Four double-stranded RNAs were detected in isolate S-0412-II 1a of M. oryzae. The buoyant densities of isometric virus particles (about 35 nm) containing these dsRNAs in CsCl ranged from 1.37-1.40 g/cm^3. It is noteworthy that the S-0412-II 1a mycovirus was detected not only in host cells but also in culture supernatant. Futhermore, abnormal aggregation of mycelia was observed after adding the mycovirus-containing culture supernatant to an uninfected strain of M. oryzae, and mycoviral dsRNAs were detectable from the aggregated mycelia.
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