Analysis on transmission of RNA silencing through grafting and its application

• Project/Area Number: 20580046
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Ehime University
• Principal Investigator: NISHIGUCHI Masamichi Ehime University, 農学部, 教授 (40343313)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: RNAサイレンシング / 遺伝子サイレンシング / ウイルス / 接ぎ木 / バイオテクノロジー / ゲノム / 遺伝子 / 農林水産物

Research Abstract

The spreading of RNA silencing occurred from 5' to 3' direction along the transgene, but not vice versa in grafted scions of transgenic Nicotiana benthamiana carrying the entire of coat protein gene (CP) of Sweet potato feathery mottle virus (SPFMV) onto the silenced rootstocks with target specificity in 3' or 5' region of SPFMC CP. The same result was obtained by crossing. The transgene methylation was induced by crossing but not by grafting. RNA silencing through grafting was also induced in non transgenic scions grafted on endogenous gene silenced rootstocks of N. tobacum.

Research Products

• [Journal Article] Spreading of RNA silencing and DNA methylation in transgenic hybrid plants with the coat protein gene of Sweet potato feathery mottle viru (2010)
  • Author(s): N.Haque, N.Yamaoka, M.Nishiguchi
  • Journal Title: Breed.Sci. 60 Pages: 361-370
  • NAID: 10027120176
  • Peer Reviewed
• [Journal Article] Analysis of rice RNA-dependent RNA polymerase 1 (OsRDR1) in virus mediated RNA silencing by particle bombardment. (2010)
  • Author(s): H.Chen, A.Tamai, M.Mori, M.Ugaki, Y.Tanaka, P.P.Samadder, A.Miyao, H.Hirochika, N.Yamaoka, M.Nishiguchi
  • Journal Title: J.Gen.Plant Pathol. 76 Pages: 152-160
  • Peer Reviewed
• [Journal Article] Spreading of RNA silencing and DNA methylation in transgenic hybrid plants with the coat protein gene of sweet potato feathery mottle virus (2010)
  • Author(s): N.Haque, N.Yamaoka, M.Nishiguchi
  • Journal Title: Breeding Science Volume: 60 Pages: 361-370
  • NAID: 10027120176
  • Peer Reviewed
• [Journal Article] Analysis of rice RNA-dependent RNA polymerase 1(OsRDR1)in virus mediated RNA silencing by particle bombardment (2010)
  • Author(s): H.Chen, et al.
  • Journal Title: J.Gen.Plant Pathol. 76 Pages: 152-160
  • Peer Reviewed
• [Journal Article] 遺伝子サイレンシングの解析とその応用 (2009)
  • Author(s): 西口正通
  • Journal Title: 愛媛ジャーナル 11月号 Pages: 80-83
• [Journal Article] 遺伝子サイレンシングの解析とその応用 (2009)
  • Author(s): 西口正通
  • Journal Title: 愛媛ジャーナル 23巻 Pages: 80-83
• [Journal Article] 植物におけるRNAサイレンシングの移行と拡大 (2008)
  • Author(s): 西口正通・A.K.M.N.Haque
  • Journal Title: 植物ウイルス病研究会レポート Pages: 1-10
• [Journal Article] 植物におけるRNAサイレンシングの移行と拡大 (2008)
  • Author(s): 西口正通
  • Journal Title: 植物ウイルス病研究会レポート. 9 Pages: 1-10
• [Journal Article] Attenuated plant viruses : a protective means to prevent virus diseases and its molecular understanding
  • Author(s): M.Nishiguchi, K.Kobayshi
  • Journal Title: J.Gen.Plant Pathol. (in press)
  • Peer Reviewed
• [Presentation] 植物におけるRNAサイレンシングとウイルス:イネイネRNA依存RNAポリメラーゼ遺伝子(OsRDR1)の解析 (2011)
  • Author(s): 西口正通
  • Organizer: 第26回中国四国ウイルス研究会
• [Presentation] Analysis of rice CHROMOMETHYLASE 3 (OsCMT3)in RNA silencing. (2010)
  • Author(s): Nishiguchi, M., et al.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-11
• [Presentation] Molecular analysis of rice RNA dependent RNA polymerase 1 (OsRDR1) in RNA silencing and antiviral pathway (2010)
  • Author(s): Nishiguchi, M., et al.
  • Organizer: 第58回日本学会学術集会ウイルス
  • Place of Presentation: 徳島市
  • Year and Date: 2010-11-09
• [Presentation] Analysis of rice genes required for RNA silencing : RNA-dependent RNA polymerase 1 (OsRDR1) (2010)
  • Author(s): Chen, H., et al.
  • Organizer: 平成22年度日本植物病理学会物関西部会
  • Place of Presentation: 福井市
  • Year and Date: 2010-09-30
• [Presentation] Analysis of RNA silencing using Brome mosaic virus in rice RNA-dependent RNA polymerase 1 (OsRDR1) mutant lines (2010)
  • Author(s): Chen, H., et al.
  • Organizer: 平成22年度日本植物病理学会
  • Place of Presentation: 京都市
  • Year and Date: 2010-04-19
• [Presentation] Characterization of Rice RNA-dependent RNA Polymerase 1(OsRDR1)in RNA silencing and antiviral pathway (2009)
  • Author(s): Chen, H., et al.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] Analysis of rice RNA-dependent RNA polymerase 1(OsRDR1)mediated RNA silencing and antiviral pathway. Abstract CD, p248(2009) (2009)
  • Author(s): Chen, H., et al.
  • Organizer: 9^<th> International Plant and Molecular Biology Congress
  • Place of Presentation: St.Louis, USA
  • Year and Date: 2009-10-27
• [Presentation] Analysis of rice RNA-dependent RNA polymerase 1(OsRDR1)in virus mediated RNA silencing. 日植病報 76(1): 73-74(2010) (2009)
  • Author(s): Chen, H., et al.
  • Organizer: 平成22年度日本植物病理学会物関西部会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-18
• [Presentation] 植物におけるRNAサイレンシングの移行と拡大 (2008)
  • Author(s): 西口正通
  • Organizer: 植物ウイルス病研究会
  • Place of Presentation: 倉敷
  • Year and Date: 2008-04-29
• [Presentation] A silencing and transgene methylation : Analysis of Transgenic Nicotiana benthamiana with the Coat Protein Gene of Sweet potato feathery mottle virus. 日植病報74(3) : 244. (2008) (2008)
  • Author(s): Haque, N. A. K. M.
  • Organizer: 平成20年度日本植物病理学会大会
  • Place of Presentation: 松江
  • Year and Date: 2008-04-27
• [Presentation] 植物におけるRNAサイレンシングの移行と拡大 (2008)
  • Author(s): 西口正通・A.K.M.N.Haque
  • Organizer: 植物ウイルス病研究会
• [Presentation] 接ぎ木によるRNAサイレンシングの移行:トバモウイルス抵抗性の付与 (2008)
  • Author(s): 玉木洋平・石川雅之・山岡直人・西口正通
  • Organizer: 植物感染生理談話会
• [Presentation] Spreading of RNA silencing and transgene methylation : analysis of transgenic Nicotiana benthamiana with the coat protein gene of Sweet potato feathery mottle virus (2008)
  • Author(s): A.K.M.N.Haque, N.Yamaoka, M.Nishiguchi
  • Organizer: 日本植物病理学会大会
• [Book] Bisulfite sequencing for cytosine-methylation analysis in plants, In : RNAi and Plant Gene Function Analysis : Methods Methods and Protocols, no. 744, Methods in Molecular Biology (eds. by H.Kodama and A.Komamine) (2011)
  • Author(s): N.Haque, M.Nishiguchi, Humana Press
• [Book] Bisulfite Sequencing for Cytosine-Methylation Analysis in Plants. In "RNAi and Plant Gene Function Analysis : Methods and Protocols"(ed.by H.Kodama and A.Komamine.) (2011)
  • Author(s): N.Haque, M.Nishiguchi
  • Total Pages: 11
  • Publisher: Humana Press(in press)
• [Book] Nova Science Publisher, RNA silencing and DNA methylation in plants, In RNA Interference Research Progress, (eds. by R.T.Lyland and I.B.Browning) (2008)
  • Author(s): A.K.M.N.Haque, M. Nishiguchi
• [Book] RNA silencing and DNA methylation in plants. In“RNA Interference Research Progress" ed. by Roger T. Lyland and Irving B. Browningr. . (2008)
  • Author(s): A. K. M. N. Haque Nazmul
  • Total Pages: 7
  • Publisher: Nova Science Publisher
• [Remarks]
  • URL: http://web.agr.ehime-u.ac.jp/~bunshishigen/index.html
• [Remarks]
  • URL: http://web.agr.ehime-u.ac.jp/?bunshishigen/index.html