Analysis on transmission of RNA silencing through grafting and its application
| Project/Area Number |
20580046
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant pathology
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| Research Institution | Ehime University |
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Principal Investigator |
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | RNAサイレンシング / 遺伝子サイレンシング / ウイルス / 接ぎ木 / バイオテクノロジー / ゲノム / 遺伝子 / 農林水産物 |
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| Research Abstract |
The spreading of RNA silencing occurred from 5' to 3' direction along the transgene, but not vice versa in grafted scions of transgenic Nicotiana benthamiana carrying the entire of coat protein gene (CP) of Sweet potato feathery mottle virus (SPFMV) onto the silenced rootstocks with target specificity in 3' or 5' region of SPFMC CP. The same result was obtained by crossing. The transgene methylation was induced by crossing but not by grafting. RNA silencing through grafting was also induced in non transgenic scions grafted on endogenous gene silenced rootstocks of N. tobacum.
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Report
(4 results)
Research Products
(28 results)
