Analysis of the signal transduction mechanism of tobacco mosaic virus resistance

• Project/Area Number: 20580049
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: SEO Shigemi National Institute of Agrobiological Sciences, 植物・微生物間相互作用研究ユニット, 主任研究員 (80414910)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 植物病理 / ウイルス / シグナル伝達 / MAPキナーゼ / ジャスモン酸 / サリチル酸 / 抵抗性 / 植物 / シグナル伝逹

Research Abstract

Mitogen-activated protein kinases (MAPKs) are signal factors that exist ubiquitously in eukaryotes. To define the exact roles of WIPK and SIPK, tobacco MAPKs, in tobacco mosaic virus (TMV) resistance, we analyzed transgenic tobacco plants in which WIPK and SIPK were silenced. Resistance to TMV was suppressed in these silenced plants, suggesting that WIPK and SIPK negatively regulate resistance to TMV, a result different from that previously shown.

Research Products

• [Journal Article] タバコモザイクウイルス抵抗性に果たすMAPキナーゼの予期せぬ役割 (2011)
  • Author(s): 小林光智衣、光原一朗、瀬尾茂美
  • Journal Title: 化学と生物 Vol.49 Pages: 226-228
• [Journal Article] タバコモザイクウイルス抵抗性に果たすMAPキナーゼの予期せぬ役割 (2011)
  • Author(s): 小林光智衣
  • Journal Title: 化学と生物 Volume: 49 Pages: 226-228
• [Journal Article] Silencing of WIPK and SIPK mitogen-activated protein kinases reduces Tobacco mosaic virus accumulation but permits systemic viral spread in tobacco possessing the N resistance gene. (2010)
  • Author(s): Kobayashi M., Seo S., Hirai K., Yamamoto-Katou A., Katou S., Seto H., Meshi T., Mitsuhara I., Ohashi Y.
  • Journal Title: Molecular Plant-Microbe Interactions(co-first authors ; corresponding author) 23 Pages: 1032-1041
  • Peer Reviewed
• [Journal Article] Silencing of WIPK and SIPK mitogen-activated protein kinases reduces Tobaccomosaic virus accumulation but permits systemic viral spread in tobacco possessing the N resistance gene (2010)
  • Author(s): Michie Kobayashi
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 23 Pages: 1032-1041
  • Peer Reviewed
• [Presentation] MAPKであるWIPKとSIPKを抑制したタバコにおけるタバコモザイクウイルス増殖抑制機構の解析 (2010)
  • Author(s): 小林光智衣
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-20
• [Presentation] タバコMAPKであるWIPKとSIPKはタバコモザイクウイルスの全身移行を阻害するがウイルス増殖は高める (2009)
  • Author(s): 瀬尾茂美
  • Organizer: 日本植物生理学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-21
• [Remarks]
  • URL: http://www.nias.affrc.go.jp/plantdefense