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Analyses of Ralstonia solanacearum type III effector proteins that can suppress plant disease resistance responses mediated by vesicular trafficking

Research Project

Project/Area Number 20580050
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant pathology
Research InstitutionResearch Institute for Biological Science Okayama

Principal Investigator

MUKAIHARA Takafumi  Research Institute for Biological Science Okayama, 専門研究員 (80344406)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords病原性因子 / 植物病原細菌 / 青枯病菌 / 病原性 / III型分泌系 / エフェクター / 小胞輸送 / 防御応答 / 植物 / タイプIII分泌系 / シロイヌナズナ
Research Abstract

Ralstonia solanacearum, the causal agent of bacterial wilt on solanaceous plants, injects virulence effector proteins into host plant cells via a type III secretion system to cause disease. In this study, we found that R. solanacearum possesses over 70 type III effectors. We constructed a series of recombinant plants and yeasts that express individual effector protein and identified effector proteins that show an ability to suppress vesicular trafficking in eukaryote cells.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (16 results)

All 2011 2010 2009 2008 Other

All Journal Article (8 results) (of which Peer Reviewed: 6 results) Presentation (8 results)

  • [Journal Article] Extracts from Ralstonia solanacearum induce effective resistance to the pathogen in both Arabidopsis and solanaceous plants.2011

    • Author(s)
      Takabatake, R., Mukaihara T.
    • Journal Title

      Journal of General Plant Pathology 77

      Pages: 33-42

    • NAID

      10029720524

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Extracts from Ralstonia solanacearum induce effective resistance to the pathogen in both Arabidopsis and solanaceous plants2011

    • Author(s)
      Reona Takabatake
    • Journal Title

      Journal of General Plant Pathology

      Volume: 77 Pages: 33-42

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome-wide identification of a large repertoire of Ralstonia solanacearum type III effector proteins by a new functional screen.2010

    • Author(s)
      Mukaihara T., Tamura N., Iwabuchi, M.
    • Journal Title

      Molecular Plant-Microbe Interactions 23

      Pages: 251-262

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gnemome-wide identification of a large repertoire of Ralstonia solanacearum type III effector proteins by a new functional screen2010

    • Author(s)
      Takafumi Mukaihara
    • Journal Title

      Molecular Plant-Microbe Interactions

      Volume: 23 Pages: 251-262

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of novel Ralstonia solanacearum type III effector proteins through translocation analysis of hrpB-regulated gene products.2009

    • Author(s)
      向原隆文, 田村尚之
    • Journal Title

      Microbiology 155

      Pages: 2235-2244

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of novel Ralstonia solanacearum type III effector proteins through translocation analysis of hrpB-regulated gene products.2009

    • Author(s)
      Takafumi Mukaihara
    • Journal Title

      Microbiology 155

      Pages: 2235-2244

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細胞融合によるナス台木の青枯病抵抗性育種2008

    • Author(s)
      田村尚之、向原隆文
    • Journal Title

      植物防疫 vol.62

      Pages: 64-67

    • Related Report
      2010 Final Research Report
  • [Journal Article] 細胞融合によるナス台木の青枯病抵抗性育種2008

    • Author(s)
      田村尚之
    • Journal Title

      植物防疫 62

      Pages: 10-13

    • Related Report
      2008 Annual Research Report
  • [Presentation] ゲノムワイドな機能的スクリーニングから見えてきた青枯病菌III型エフェクター群の全貌2009

    • Author(s)
      向原隆文
    • Organizer
      平成21年度日本植物病理学会大会
    • Place of Presentation
      山形
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] 青枯病菌 Ralstonia solanacearum のIII型分泌系を介した植物感染機構2009

    • Author(s)
      向原隆文
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] 枯病菌Ralstonia solanacearumのIII型分泌系を介した植物感染機構2009

    • Author(s)
      向原隆文
    • Organizer
      第82回日本細菌学会総会シンポジウム
    • Place of Presentation
      名古屋
    • Related Report
      2010 Final Research Report
  • [Presentation] ゲノムワイドな機能的スクリーニングから見えてきた青枯病菌III型エフェクター群の全貌2009

    • Author(s)
      向原隆文
    • Organizer
      平成21年度日本植物病理学会大会課題別シンポジウム
    • Place of Presentation
      山形
    • Related Report
      2010 Final Research Report
  • [Presentation] 青枯病菌の菌体成分による植物の基礎的抵抗性反応の誘導2008

    • Author(s)
      高畠令王奈
    • Organizer
      平成20年度日本植物病理学会大会
    • Place of Presentation
      松江
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] 新規手法による青枯病菌III型エフェクター遺伝子の機能的スクリーニング2008

    • Author(s)
      向原隆文
    • Organizer
      平成20年度日本植物病理学会大会
    • Place of Presentation
      松江
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] 青枯病菌の菌体成分による植物免疫反応の誘導およびエフェクターによる抑制2008

    • Author(s)
      高畠令王奈
    • Organizer
      2008年日本分子生物学会年会・日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] 真核細胞の小胞輸送を阻害する青枯病菌Ralstonia solanacearumタイプIIIエフェクターの同定

    • Author(s)
      高畠令王奈
    • Organizer
      第49回日本植物生理学会
    • Place of Presentation
      札幌
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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