Molecular characterization of the TIA-1 like RNA binding protein of the silk worm, Bombyx mori.

• Project/Area Number: 20580051
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: KOTANI Eiji Kyoto Institute of Technology, 工芸科学研究科, 准教授 (10273541)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: チョウ目昆虫 / NA結合蛋白質 / 転写産物制御 / RNA結合蛋白質 / RNA結合タンパク質 / カイコ / タンパク質生産 / ウイルス抵抗性

Research Abstract

BmTRN-1 from the silkworm, Bombyx mori, is an RNA-binding protein homologous to mammalian TIA-1/TIAR, that possesses three RNA recognition motifs (RRMs) followed by a C-terminal auxiliary domain. Previous studies showed that the function of the BmTRN-1 is possibly involved in several mechanisms of RNA metabolism including the regulation of transcripts in the Bombyx cultured cells. In this study, the RRM2 and RRM1 domain of BmTRN-1 were found to be related to its nuclear export and nuclear accumulation, respectively. Also, Analyses by using the expressed GFP-BmTRN-1 fusion protein in the cells indicated that the BmTRN-1 distribution is drastically changed, and further indicated that the protein is distributed in the cytoplasm and forms aggregate structure, especially when the cells are infected by a baculovirus, Bombyx mori nucleopolyhedrovirus (BmNPV). Protein expression level of a BmNPV reporter protein was shown to be altered both by the BmTRN-1-overexpression and BmTRN-1-knockdown in the cultured cells and the transgenic individual tissues. In addition, we found that BmTRN-1 interacts with one of the BmNPV's nucleic acid-binding protein and the C-terminal auxiliary domain of BmTRN-1 is important for its interaction with the viral protein. Thus, it was indicated from these findings that the interaction between BmTRN-1 and the viral protein relates to the possible cellular function of resisting baculovirus proliferation.

Research Products

• [Journal Article] Immobilization of protein kinase C into cypovirus polyhedra. (2010)
  • Author(s): Ijiri, H., Nakatani, T., Ido, H., Hamada, N., Kotani, E., Mori, H.
  • Journal Title: J.Insect Biotech. Sericol. 79 Pages: 15-20
  • NAID: 10026132179
  • Peer Reviewed
• [Journal Article] Immobilization of protein kinase C into cypovirus polyhedra. (2010)
  • Author(s): Ijiri, H., Nakatani, T., Ido, H., Hamada, N., Kotani, E., Mori, H.
  • Journal Title: Journal of.Insect Biotechnology and Sericology. Volume: 79 Pages: 15-20
  • NAID: 10026132179
  • Peer Reviewed
• [Journal Article] Structure-based targeting of bioactive proteins into cypovirus polyhedra and application to immobilized-cytokines for mammalian cell culture. (2009)
  • Author(s): Ijiri, H., Coulibaly, F., Nishimura, G., Nakai, D., Chiu, E., Takenaka, C., Ikeda, K., Nakazawa, H., Hamada, N., Kotani, E., Metcalf, P., Kawamata, S., Mori, H.
  • Journal Title: Biomaterials 30 Pages: 4297-4308
  • Peer Reviewed
• [Journal Article] Functional analysis of the gene of BmTRN-1, an RNA-binding protein homologous to mammalian TIA-1 from the silkworm, Bombyx mori ; a study of it's overexpression and sub-cellular distribution during the baculovirus infection process. (2009)
  • Author(s): Muto, S., Matsumoto, E., Tanabe, T., Mori, H., Kotani, E.
  • Journal Title: J.Insect Biotech. Sericol. 78 Pages: 39-51
  • NAID: 10024833667
  • Peer Reviewed
• [Journal Article] Molecular Characterization of a TIA-1 like RNA-binding protein in the cells derived from the fall armyworm, Spodoptera frugiperda(Lepidoptera : Noctuidae). (2009)
  • Author(s): Muto, S., Tanabe, T., Matsumoto, E., Mori, H., Kotani, E.
  • Journal Title: Bioscience Biotechnology and Biochemistry 73 Pages: 648-656
  • NAID: 10027538769
  • Peer Reviewed
• [Journal Article] Functional analysis of the gene of BmTRN-1, an RNA-binding protein homologous to mammalian TIA-1 from the silkworm, Bombyx mori, by its overexpression and subcellular distribution study during the baculovirus infection process (2009)
  • Author(s): Muto, S., Matsumoto, E., Tanabe, T., Mori, H., Kotani, E.
  • Journal Title: Journal of Insect Biotechnology and Sericology 78 Pages: 39-51
  • Peer Reviewed
• [Journal Article] Structure-based targeting of bioactive proteins into cypovirus polyhedra and application to immobilized cytokines for mammalian cell culture (2009)
  • Author(s): Ijiri H, Coulibaly F, Nishimura G, Nakai D, Chiu E, Takenaka C, Ikeda K, Nakazawa H, Hamada N, Kotani E, Metcalf P, Kawamata S, Mori H.
  • Journal Title: Biomaterials 30 Pages: 4297-4308
  • Peer Reviewed
• [Journal Article] Molecular Characterization of a TIA-1-like RNA-binding protein in cells derived from the fall armyworm, Spodoptera frugiperda(Lepidoptera : Noctuidae) (2009)
  • Author(s): Muto, S., Tanabe, T., Matsumoto, E., Mori, H., Kotani, E
  • Journal Title: Bioscience, Biotechnology and Biochemistry 73 Pages: 648-656
  • NAID: 10027538769
  • Peer Reviewed
• [Presentation] ウイルス感染に伴うカイコTIA-1相同性タンパク質の細胞内分布変化の解析 (2010)
  • Author(s): 小谷英治・武藤清佳・福嶋真実・森肇
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県神戸市)
  • Year and Date: 2010-12-09
• [Presentation] ウイルス感染に伴うカイコTIA-1相同性タンパク質の細胞内分布変化の解析 (2010)
  • Author(s): 小谷英治
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] カイコのTIA-1ホモローグ(BmTRN-1)と相互作用するタンパク質の検索 (2010)
  • Author(s): 小谷英治・武藤清佳・福嶋真実・森肇
  • Organizer: 平成22年度日本蚕糸学会支部合同大会
  • Place of Presentation: てだこホール(沖縄県浦添市)
  • Year and Date: 2010-11-14
• [Presentation] バキュロウイルス感染によるカイコRNA結合タンパク質(BmTRN-1)の局在変化 (2010)
  • Author(s): 武藤清佳, 松本恵実, 田辺徹, 中村仁, 山本賢二, 森肇, 小谷英治
  • Organizer: 平成22年度日本蚕糸学会第80回記念大会蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 信州大学
  • Year and Date: 2010-04-04
• [Presentation] バキュロウイルス感染によるカイコRNA結合タンパク質(BmTRN-1)の局在変化 (2010)
  • Author(s): 武藤清佳・松本恵実・田辺徹・中村仁・山本賢二・森肇・小谷英治
  • Organizer: 平成22年度蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 信州大学繊維学部(長野県上田市)
  • Year and Date: 2010-04-03
• [Presentation] カイコ細胞におけるTIA-1様RNA結合タンパク質の機能解析 (2009)
  • Author(s): 小谷英治, 武藤清佳, 森肇
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-12-10
• [Presentation] カイコ細胞におけるTIA-1様RNA結合タンパク質の機能解析 (2009)
  • Author(s): 小谷英治, 武藤清佳, 森肇
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] カイコ細胞におけるRNA結合タンパク質(BmTRN-1)の細胞内分布の解析 (2009)
  • Author(s): 武藤清佳, 松本恵実, 田辺徹, 森肇, 小谷英治
  • Organizer: 平成21年度蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 東京農工大
  • Year and Date: 2009-03-23