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Molecular identification and characterization of sperm maturation factors in lepidopteran insect.

Research Project

Project/Area Number 20580052
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied entomology
Research InstitutionKyoto Institute of Technology

Principal Investigator

NAGAOKA Sumiharu  Kyoto Institute of Technology, 工芸科学研究科, 助教 (00303933)

Co-Investigator(Kenkyū-buntansha) SHIRAI Kohji  信州大学, 繊維学部, 准教授 (00293499)
YAMAMOTO Kohji  九州大学, 農学研究科, 助教 (00346834)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords昆虫 / 酵素 / 生理活性 / 発生・分化 / プロテオーム
Research Abstract

Molecular activation mechanisms of sperm maturation in insects have been studied mainly in the Lepidoptera. In the male silkworm, Bombyx mori, secretions of the prostatic gland which contain trypsin like a serine-type endopeptidase called initiatorin, trigger a cascade reaction that leads to induction of sperm maturation. Here, we identified initiatorin and showed that it efficiently cleaves fluorogenic peptides with a dibasic sequence. Similar processes of endopeptidase-triggered initiation of motility were found in Cephonodes hylas. Moreover, we provide evidence that the action of arginase (AG), nitric oxide synthase (NOS) and di-peptidylpeptidase (angiotensin converting enzyme, ACE) mediates a step for Bombyx sperm maturation.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (58 results)

All 2011 2010 2009 2008

All Journal Article (23 results) (of which Peer Reviewed: 20 results) Presentation (30 results) Book (3 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Identification of two arginases generated by alternative splicing in the silkworm2011

    • Author(s)
      Nagaoka S., Takata Y., Kato K.
    • Journal Title

      Bombyx mori., Arch.Insect Biochem. 76

      Pages: 97-113

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of two arginases generated by alternative splicing in the silkworm, Bombyx mori.2011

    • Author(s)
      Nagaoka, S.
    • Journal Title

      Arch.Insect Biochem.

      Volume: 76 Pages: 97-113

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of the powder of porous titanium carbide ceramics to a reusable adsorbent for environmental pollutants.2011

    • Author(s)
      Moriwaki, H.
    • Journal Title

      J.Hazard.Mater.

      Volume: 185 Pages: 725-731

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of diazinon exposure on antioxidant reactions in the silkmoth, Bombyx mori.2011

    • Author(s)
      Yamamoto, K.
    • Journal Title

      J Appl.Entomol.

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization of an insecticide-induced novel glutathione-transferase in silkworm.2011

    • Author(s)
      Yamamoto, K.
    • Journal Title

      Biochim Biophys Acta.

      Volume: 1810 Pages: 420-426

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Calcium oxalate crystals in mulberry leaves : No negative effect on feeding the silkworm2010

    • Author(s)
      Nagaoka S., Katayama H., Fujibayashi Y., Sugimura Y.
    • Journal Title

      Bombyx mori.J.Insect Biotechnol. Sericol. 79

      Pages: 71-74

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Calcium oxalate crystals in mulberry leaves : No negative effect on feeding the silkworm, Bombyx mori.2010

    • Author(s)
      Nagaoka, S.
    • Journal Title

      J.Insect Biotechnol.Sericol.

      Volume: 79 Pages: 71-74

    • NAID

      10026709215

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characteristics of cellular morphology associated with dermal knob formation in the knobbed mutant (K) of the silkworm, Bombyx mori.2010

    • Author(s)
      Shimura, S.
    • Journal Title

      J.Insect Biotechnol.Sericol.

      Volume: 79 Pages: 21-25

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and expression analysis of minichromosome maintenance proteins in the silkworm, Bombyx mori2010

    • Author(s)
      Yamamoto, K.
    • Journal Title

      J.Insect Science

      Volume: 10 Pages: 48-48

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression analysis of cytochrome P450s in the silkmoth, Bombyx mori.2010

    • Author(s)
      Yamamoto, K.
    • Journal Title

      Pestic.Biochem.Phys.

      Volume: 97 Pages: 1-6

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural properties of silkworm small heat-shock proteins : sHSP 19.9 and sHSP20.8.2010

    • Author(s)
      Hossain, MD.T.
    • Journal Title

      Biosci.Biotech.Biochem.

      Volume: 74 Pages: 1556-1563

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biochemical properties of an omega-class glutathione S-transferase of the silkmoth2009

    • Author(s)
      Yamamoto K., Nagaoka S., Banno Y., Aso Y.
    • Journal Title

      Bombyx mori.Comp.Biochem. Phys. 149C

      Pages: 461-467

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Introduction to the proposed space experiments aboard the ISS using the silkworm, Bombyx mori2009

    • Author(s)
      Furusawa, T.
    • Journal Title

      Biol.Sci.Space 23

      Pages: 61-69

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Larval labial glands of the sweet potato hornworm, Agrius convolvuli : morphology and secretory proteins2009

    • Author(s)
      Zha X.-M.
    • Journal Title

      J.Insect Biotechnol.Sericol. 78

      Pages: 53-60

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of lumen proteins from the labial glands of 5th instar larvae of Agrius convolvuli (Lepidoptera : Sphingidae)2009

    • Author(s)
      Zha X.-M.
    • Journal Title

      J.Insect Biotechnol.Sericol. 78

      Pages: 53-60

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Switchover of protein systhesis occurs during the wandering stage in the labial glands of the sweet potato hornworm, Agrius convolvuli2009

    • Author(s)
      Shirai K.
    • Journal Title

      J.Insect Biotechnol.Sericol. 78

      Pages: 107-112

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Utilization of silkworm cocoon waste as a sorbent for the removal of oil from water2009

    • Author(s)
      Moriwaki, H.
    • Journal Title

      J.Hazard.Mater. 165

      Pages: 266-270

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biochemical properties of an omega-class glutathione S-transferase of the silkmoth, Bombyx mori2009

    • Author(s)
      Yamamoto, K.
    • Journal Title

      Comp.Biochem.Physiol. 149C

      Pages: 461-467

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of glutathione S-transferase of the saturniid moth, Samia cynthia pryeri (Lepidoptera : Saturniidae)2009

    • Author(s)
      Yamamoto, K.
    • Journal Title

      J.Appl.Entomol. 133

      Pages: 278-283

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular and biochemical characterization of a Zeta-class glutathione S-transferase of the silkmoth2009

    • Author(s)
      Yamamoto, K.
    • Journal Title

      Pest.Biochem.Physiol. 95

      Pages: 125-128

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      長岡純治
    • Journal Title

      虫たちが語る生物学の未来(衣笠会)

      Pages: 114-119

    • Related Report
      2009 Annual Research Report
  • [Journal Article]2009

    • Author(s)
      白井孝治
    • Journal Title

      虫たちが語る生物学の未来(衣笠会)

      Pages: 180-183

    • Related Report
      2009 Annual Research Report
  • [Journal Article]2009

    • Author(s)
      古澤壽治, 他, 長岡純治
    • Journal Title

      虫たちが語る生物学の未来(衣笠会)

      Pages: 81-81

    • Related Report
      2009 Annual Research Report
  • [Presentation] 昆虫精子の活性化におけるトリプシン様セリンプロテアーゼの存在2010

    • Author(s)
      長岡純治・藤田菜津子・大槻正嗣
    • Organizer
      日本蚕糸学会合同支部大会
    • Place of Presentation
      浦添市
    • Year and Date
      2010-11-15
    • Related Report
      2010 Final Research Report
  • [Presentation] 昆虫精子の活性化におけるトリプシン様セリンプロテアーゼの存在2010

    • Author(s)
      長岡純治
    • Organizer
      日本蚕糸学会合同支部大会
    • Place of Presentation
      てだこホール(浦添)
    • Year and Date
      2010-11-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 昆虫無細胞タンパク質合成系を用いた有機溶媒耐性チロシナーゼ活性化機構の解析2010

    • Author(s)
      伊東昌章
    • Organizer
      日本蚕糸学会合同支部大会
    • Place of Presentation
      てだこホール(浦添)
    • Year and Date
      2010-11-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 「きぼう」での宇宙放射線研究Rad Silk報告:宇宙放射線によるカイコでの突然変異発現2010

    • Author(s)
      古澤壽治
    • Organizer
      日本放射線影響学会 第53回大会
    • Place of Presentation
      京都テルサ(京都)
    • Year and Date
      2010-10-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 宇宙放射線によるカイコでの突然変異発現2010

    • Author(s)
      古澤壽治
    • Organizer
      日本宇宙生物科学会 第24回大会
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2010-09-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] The identification of the sperm activating factor, initiatorin, which is a prostatic endopeptidase of the silkworm, Bombyx mori.2010

    • Author(s)
      Nagaoka S., Kato K., Takata Y.
    • Organizer
      11^<th> International symposium on spermatology.
    • Place of Presentation
      宜野湾市
    • Year and Date
      2010-06-26
    • Related Report
      2010 Final Research Report
  • [Presentation] The identification of the sperm activating factor, initiatorm, which is a prostatic endopeptidase of the silkworm, Bombyx mori.2010

    • Author(s)
      長岡純治
    • Organizer
      11^<th> International Symposium on Spermatology
    • Place of Presentation
      沖縄コンベンションセンター(宜野湾)
    • Year and Date
      2010-06-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] シュウ酸カルシウムのカイコ摂食性に及ぼす影響2010

    • Author(s)
      杉村順夫
    • Organizer
      日本蚕糸学会 第80回大会
    • Place of Presentation
      信州大学繊維学部(上田)
    • Year and Date
      2010-04-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] カイコガ生殖腺分泌物に含まれるAngiotensin-converting enzymeの存在とその機能2010

    • Author(s)
      長岡純治・河崎紗織
    • Organizer
      日本蚕糸学会第80回大会
    • Place of Presentation
      上田市
    • Year and Date
      2010-04-03
    • Related Report
      2010 Final Research Report
  • [Presentation] カイコガ生殖腺分泌物に含まれるAngiotensin-converting enzymeの存在とその機能2010

    • Author(s)
      長岡純治
    • Organizer
      日本蚕糸学会 第80回大会
    • Place of Presentation
      信州大学繊維学部(上田)
    • Year and Date
      2010-04-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] エビガラスズメ緑色幼虫の体色発現機構:色素顆粒中のiNS凝集成分Xについて2010

    • Author(s)
      白井孝治
    • Organizer
      日本蚕糸学会 第80回大会
    • Place of Presentation
      信州大学繊維学部(上田)
    • Year and Date
      2010-04-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] 熱重量分析法を用いた繭の透湿性測定2010

    • Author(s)
      金子文俊
    • Organizer
      日本蚕糸学会 第80回大会
    • Place of Presentation
      信州大学繊維学部(上田)
    • Year and Date
      2010-04-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] カイコの摂食行動モチベーションを調節する新規ペプチド(HemaP)の作用機構2010

    • Author(s)
      諸岡信克
    • Organizer
      日本蚕糸学会 第80回大会
    • Place of Presentation
      信州大学繊維学部(上田)
    • Year and Date
      2010-04-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] 重イオン照射造血器官の崩壊・再生機構:血球前駆細胞の重イオン照射に対する応答2010

    • Author(s)
      深本花菜
    • Organizer
      日本蚕糸学会 第80回大会
    • Place of Presentation
      信州大学繊維学部(上田)
    • Year and Date
      2010-04-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] 農薬応答性グルタチオン転移酵素2010

    • Author(s)
      山本幸治
    • Organizer
      日本蚕糸学会 第80回大会
    • Place of Presentation
      信州大学繊維学部(上田)
    • Year and Date
      2010-04-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] 新規なカイコ由来グルタチオン転移酵素の発現ならびに機能解析2010

    • Author(s)
      山本幸治
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京 (東京)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 培地へのフラーレン添加が昆虫培養細胞 (Sf9) に与える影響2009

    • Author(s)
      赤崎徹志
    • Organizer
      日本蚕糸学会中部支部・東海支部合同研究発表会
    • Place of Presentation
      名古屋 (愛知)
    • Year and Date
      2009-12-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] カイコにおける抗菌剤ジンクピリチオンの胚毒性2009

    • Author(s)
      倉島征大
    • Organizer
      日本蚕糸学会中部支部・東海支部合同研究発表会
    • Place of Presentation
      名古屋 (愛知)
    • Year and Date
      2009-12-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] 重イオンビーム照射カイコ造血器官の崩壊・再生機構の解明-低線量照射における影響-2009

    • Author(s)
      小林智史
    • Organizer
      日本蚕糸学会中部支部・東海支部合同研究発表会
    • Place of Presentation
      名古屋 (愛知)
    • Year and Date
      2009-12-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒアルロン酸スポンジの家蚕血球に対する親和性2009

    • Author(s)
      石田拓郎
    • Organizer
      日本蚕糸学会中部支部・東海支部合同研究発表会
    • Place of Presentation
      名古屋 (愛知)
    • Year and Date
      2009-12-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] 家蚕幼虫体液の熱凝集タンパク質2009

    • Author(s)
      倉掛麻衣子
    • Organizer
      日本蚕糸学会関西支部・九州支部合同研究発表会
    • Place of Presentation
      名古屋 (愛知)
    • Year and Date
      2009-12-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] 新規カイコ・シトクロムP450の同定ならびに発現解析2009

    • Author(s)
      山本幸治
    • Organizer
      日本農芸化学会支部合同大会
    • Place of Presentation
      沖縄 (沖縄)
    • Year and Date
      2009-10-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] Stability of silkworm small heat shock proteins2009

    • Author(s)
      Md.Tofazzal Hossain
    • Organizer
      日本農芸化学会支部合同大会
    • Place of Presentation
      沖縄 (沖縄)
    • Year and Date
      2009-10-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] カイコにおける薬剤応答因子の探索2009

    • Author(s)
      山本幸治
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸 (兵庫)
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] 家蚕スモールヒートショックタンパク質・熱変性タンパク質複合体形成反応の特性2009

    • Author(s)
      手柴智史
    • Organizer
      化学関連支部合同九州学会
    • Place of Presentation
      北九州 (福岡)
    • Year and Date
      2009-07-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] カイコ由来オメガクラス・グルタチオン転移酵素の発現ならびに機能解析2009

    • Author(s)
      山本幸治
    • Organizer
      日本農芸化学会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] エビガラスズメ幼虫真皮細胞における真皮細胞カロチノイド結合タンパク質〜分泌顆粒中における局在〜2009

    • Author(s)
      福島壽斗
    • Organizer
      日本蚕糸学会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] カイコ・シトクロムP450の構造解析、発現系の構築ならびに基質の探索2009

    • Author(s)
      山本幸治
    • Organizer
      日本蚕糸学会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] 鱗翅目昆虫グルタチオン転移酵素群の機能解析2008

    • Author(s)
      山本幸治
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] デハロゲナーゼ活性を有するカイコ・グルタチオン転移酵素.2008

    • Author(s)
      山本幸治
    • Organizer
      日本農芸化学会西日本支部会
    • Place of Presentation
      長崎
    • Year and Date
      2008-09-20
    • Related Report
      2008 Annual Research Report
  • [Book] バイオマスとその利用(応用生物学入門)2010

    • Author(s)
      山本博規・白井孝治
    • Total Pages
      22
    • Publisher
      オーム社
    • Related Report
      2010 Annual Research Report
  • [Book] 虫たちが語る生物学の未来(精子が動き出す仕組み)2009

    • Author(s)
      長岡純治
    • Publisher
      衣笠会
    • Related Report
      2010 Final Research Report
  • [Book] Plant-herbivore interaction with special reference to mulberry leaves and the silkworm, Bombyx mori2008

    • Author(s)
      Sugimura, Y., Nagaoka, S.
    • Publisher
      Nova Science Publishers
    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] 無細胞系タンパク質合成用抽出液の調整方法、およびカイコ組織用抽出キット2009

    • Inventor(s)
      長岡純治・東出将賢・伊東昌章
    • Industrial Property Rights Holder
      国立大学法人京都工芸繊維大学, 株式会社島津製作所
    • Acquisition Date
      2009-01-20
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 無細胞系タンパク質合成用抽出液の調整方法、およびカイコ組織用抽出キット2009

    • Inventor(s)
      長岡純治, 東出将賢, 伊東昌章
    • Industrial Property Rights Holder
      京都工芸繊維大学, 株式会社島津製作所
    • Acquisition Date
      2009-01-16
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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