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Functional analysis of an alcohol acetyltransferase gene involved in sex pheromone biosynthesis

Research Project

Project/Area Number 20580058
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied entomology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

MOTO Ken-Ichi  The Institute of Physical and Chemical Research, 松本分子昆虫学研究室, 専任研究員 (90333335)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsアルコールアセチルトランスフェラーゼ / アワヨトウ / カイコガ / 性フェロモン / EST / cDNAライブラリー / EST解析 / フェロモン腺
Research Abstract

To isolate a novel alcohol acetyltransferase (AAT) gene involved in sex pheromone biosynthesis from of army worm, Pseudaletia separata, 1,000 pheromone gland EST clones were analyzed. Since the silkmoth, Bombyx mori, has no AAT, the pheromone gland EST colnes of P. separata were compared with that of B. mori. Finally, 3 novel genes were isolated as AAT candidates.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (22 results)

All 2011 2010 2009 2008 Other

All Journal Article (6 results) (of which Peer Reviewed: 4 results) Presentation (14 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Studies of sex pheromone production under neuroendocrine control by analytical and morphological means in the oriental armyworm, Pseudaletia separata, Walker (Lepidoptera : Noctuidae).2011

    • Author(s)
      Fonagy A., Moto K., Ohnishi A., Kurihara M., Kis J., Matsumoto S.
    • Journal Title

      Gen Comp Endocrinol. 172

      Pages: 62-76

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 蛾類昆虫の性フェロモンとその生合成メカニズム2010

    • Author(s)
      本賢一, 他
    • Journal Title

      ビオフィリア

      Volume: 23 Pages: 75-80

    • NAID

      40017303401

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Pheromone-gland-specific fatty-acyl reductase in the adzuki bean borer, Ostrinia scapulalis (Lepidoptera : Crambidae).2009

    • Author(s)
      Binu A., Fujii T., Moto K., Matsumoto S., Fukusawa M., Tatsuki S., Ishikawa Y.
    • Journal Title

      Insect Biochem.Molec.Biol. 39

      Pages: 90-95

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] NMR solution structure analysis of the C-terminal lenear and cyclic prptides of pheromone biosynthesis-activating neuropeptide(PBAN)from the silkmoth Bombyx mori2009

    • Author(s)
      Nagata, K., et al.
    • Journal Title

      Biopolymers 92

      Pages: 335-335

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] NMR solution structure analysis of the active and inactive fragments of pheromone biosynthesis-activating neuropeptide (PBAN) from the silkmoth Bombyx mori2008

    • Author(s)
      Nagata K., et al.
    • Journal Title

      J Pept Sci. 14

      Pages: 168-168

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Pheromone-gland-specific fatty-acyl reductase in the adzuki bean borer, Ostrinia scapulalis (Lepidoptera : Crambidae)

    • Author(s)
      Antony B., et al.
    • Journal Title

      Insect Biochem Mol Biol.

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] アワヨトウ性フェロモン生合成に関わるアシル基還元酵素遺伝子の単離と機能解析2011

    • Author(s)
      本賢一、李載みん、栗原政明、永田宏次、田之倉優、長澤寛道、松本正吾
    • Organizer
      第55回日本応用動物昆虫学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2011-03-29
    • Related Report
      2010 Final Research Report
  • [Presentation] アワヨトウ性フェロモン生合成に関わるアシル基還元酵素遺伝子の単離と機能解析2011

    • Author(s)
      本賢一, 他
    • Organizer
      日本応用動物昆虫学会
    • Place of Presentation
      福岡
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] Studies of sex pheromone production under neuroendocrine control by analytical and morphological means in the oriental armyworm, Pseudaletia separata, Walker (Lepidoptera : Noctuidae)2010

    • Author(s)
      Adrien F., Moto K., Ohnishi A., Matsumoto S.
    • Organizer
      25th Conference of European comparative endocrinologists.
    • Place of Presentation
      Pecs, Hungary
    • Year and Date
      2010-09-03
    • Related Report
      2010 Final Research Report
  • [Presentation] Studies of sex pheromone production under neuroendocrine control by analytical and morphological means in the oriental armyworm, Pseudaletia separata, Walker (Lepidoptera : Noctuidae).2010

    • Author(s)
      Adrien F., et al.
    • Organizer
      25th Conference of European comparative endocrinologists
    • Place of Presentation
      Pecs, Hungary
    • Year and Date
      2010-09-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] Molecular analysis of sex pheromone production-related gene in the silkmoth, Bombyx mori.2010

    • Author(s)
      Moto K., et al.
    • Organizer
      9th European congress of entomology
    • Place of Presentation
      Budapest, Hungary
    • Year and Date
      2010-08-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Molecular analysis of sex pheromone production-related gene in the silkmoth, Bombyx mori.2010

    • Author(s)
      Moto K., Matsumoto S.
    • Organizer
      9th European congress of entomology.
    • Place of Presentation
      Budapest, Hungary
    • Year and Date
      2010-08-25
    • Related Report
      2010 Final Research Report
  • [Presentation] アワヨトウ性フェロモン生合成に関わるアシル基還元酵素遺伝子の単離2010

    • Author(s)
      本賢一、李載みん、栗原政明、永田宏次、田之倉優、長澤寛道、松本正吾
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-30
    • Related Report
      2010 Final Research Report
  • [Presentation] アワヨトウ性フェロモン生合成に関わるアシル基還元酵素遺伝子の単離2010

    • Author(s)
      本賢一, 他
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 害虫の繁殖抑制に応用可能なリガンドと受容体膜タンパク質の機能解析:フェロモン産生関連分子の遺伝子および機能解析2010

    • Author(s)
      李載〓、本賢一、大西敦、永峰俊弘、田之倉優、長澤寛道、永田宏次、松本正吾
    • Organizer
      [平成22年度]ターゲットタンパク研究プログラムから見える未来3
    • Place of Presentation
      東京
    • Year and Date
      2010-03-05
    • Related Report
      2010 Final Research Report
  • [Presentation] カイコガ性フェロモン生合成活性化神経ペプチド(PBAN)の活性型立体構造の解析2009

    • Author(s)
      永田宏次、岡田晃季、河合岳志、大塚淳、Jimmy Joe Hull、本賢一、松本正吾、長澤寛道、田之倉優
    • Organizer
      第9回日本蛋白質科学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2009-05-22
    • Related Report
      2010 Final Research Report
  • [Presentation] アワヨトウ性フェロモン生合成に関わる脂肪酸不飽和化酵素2009

    • Author(s)
      本賢一、栗原政明、李載〓、永田宏次、長澤寛道、松本正吾
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-29
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] カイコガPBAN受容体(PBANR)アイソフォームの同定とその遺伝子発現解析2009

    • Author(s)
      李載〓、Jimmy Joe Hull、本賢一、大西敦、永田宏次、長澤寛道、松本正吾
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-29
    • Related Report
      2010 Final Research Report
  • [Presentation] カイコガ性フェロモン生合成活性化神経ペプチド(PBAN)の環状アゴニストのNMR構造解析2009

    • Author(s)
      永田宏次、岡田晃季、河合岳志、大塚淳、Jimmy Joe Hull、本賢一、松本正吾、長澤寛道、田之倉優
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-29
    • Related Report
      2010 Final Research Report
  • [Presentation] 害虫の繁殖抑制に応用可能なリガンドと受容体膜タンパク質の構造・機能解析-ガ類昆虫の性フェロモン産生関連遺伝子の単離と機能解析-2009

    • Author(s)
      本賢一、李載〓、大西敦、Jimmy Joe Hull、長澤寛道、永田宏次、松本正吾
    • Organizer
      [平成20年度]ターゲットタンパク研究プログラムから見える未来2
    • Place of Presentation
      東京
    • Year and Date
      2009-01-15
    • Related Report
      2010 Final Research Report
  • [Book] 蛾類昆虫の性フェロモンとその生合成メカニズム2010

    • Author(s)
      本賢一、松本正吾
    • Publisher
      ビオフィリア
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ

    • URL

      http://www.riken.jp/insect/

    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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