Functional analysis of an alcohol acetyltransferase gene involved in sex pheromone biosynthesis

• Project/Area Number: 20580058
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: MOTO Ken-Ichi The Institute of Physical and Chemical Research, 松本分子昆虫学研究室, 専任研究員 (90333335)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: アルコールアセチルトランスフェラーゼ / アワヨトウ / カイコガ / 性フェロモン / EST / cDNAライブラリー / EST解析 / フェロモン腺

Research Abstract

To isolate a novel alcohol acetyltransferase (AAT) gene involved in sex pheromone biosynthesis from of army worm, Pseudaletia separata, 1,000 pheromone gland EST clones were analyzed. Since the silkmoth, Bombyx mori, has no AAT, the pheromone gland EST colnes of P. separata were compared with that of B. mori. Finally, 3 novel genes were isolated as AAT candidates.

Research Products

• [Journal Article] Studies of sex pheromone production under neuroendocrine control by analytical and morphological means in the oriental armyworm, Pseudaletia separata, Walker (Lepidoptera : Noctuidae). (2011)
  • Author(s): Fonagy A., Moto K., Ohnishi A., Kurihara M., Kis J., Matsumoto S.
  • Journal Title: Gen Comp Endocrinol. 172 Pages: 62-76
  • Peer Reviewed
• [Journal Article] 蛾類昆虫の性フェロモンとその生合成メカニズム (2010)
  • Author(s): 本賢一, 他
  • Journal Title: ビオフィリア Volume: 23 Pages: 75-80
  • NAID: 40017303401
• [Journal Article] Pheromone-gland-specific fatty-acyl reductase in the adzuki bean borer, Ostrinia scapulalis (Lepidoptera : Crambidae). (2009)
  • Author(s): Binu A., Fujii T., Moto K., Matsumoto S., Fukusawa M., Tatsuki S., Ishikawa Y.
  • Journal Title: Insect Biochem.Molec.Biol. 39 Pages: 90-95
  • Peer Reviewed
• [Journal Article] NMR solution structure analysis of the C-terminal lenear and cyclic prptides of pheromone biosynthesis-activating neuropeptide(PBAN)from the silkmoth Bombyx mori (2009)
  • Author(s): Nagata, K., et al.
  • Journal Title: Biopolymers 92 Pages: 335-335
  • Peer Reviewed
• [Journal Article] NMR solution structure analysis of the active and inactive fragments of pheromone biosynthesis-activating neuropeptide (PBAN) from the silkmoth Bombyx mori (2008)
  • Author(s): Nagata K., et al.
  • Journal Title: J Pept Sci. 14 Pages: 168-168
• [Journal Article] Pheromone-gland-specific fatty-acyl reductase in the adzuki bean borer, Ostrinia scapulalis (Lepidoptera : Crambidae)
  • Author(s): Antony B., et al.
  • Journal Title: Insect Biochem Mol Biol.
  • Peer Reviewed
• [Presentation] アワヨトウ性フェロモン生合成に関わるアシル基還元酵素遺伝子の単離と機能解析 (2011)
  • Author(s): 本賢一、李載みん、栗原政明、永田宏次、田之倉優、長澤寛道、松本正吾
  • Organizer: 第55回日本応用動物昆虫学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2011-03-29
• [Presentation] アワヨトウ性フェロモン生合成に関わるアシル基還元酵素遺伝子の単離と機能解析 (2011)
  • Author(s): 本賢一, 他
  • Organizer: 日本応用動物昆虫学会
  • Place of Presentation: 福岡
  • Year and Date: 2011-03-29
• [Presentation] Studies of sex pheromone production under neuroendocrine control by analytical and morphological means in the oriental armyworm, Pseudaletia separata, Walker (Lepidoptera : Noctuidae) (2010)
  • Author(s): Adrien F., Moto K., Ohnishi A., Matsumoto S.
  • Organizer: 25th Conference of European comparative endocrinologists.
  • Place of Presentation: Pecs, Hungary
  • Year and Date: 2010-09-03
• [Presentation] Studies of sex pheromone production under neuroendocrine control by analytical and morphological means in the oriental armyworm, Pseudaletia separata, Walker (Lepidoptera : Noctuidae). (2010)
  • Author(s): Adrien F., et al.
  • Organizer: 25th Conference of European comparative endocrinologists
  • Place of Presentation: Pecs, Hungary
  • Year and Date: 2010-09-03
• [Presentation] Molecular analysis of sex pheromone production-related gene in the silkmoth, Bombyx mori. (2010)
  • Author(s): Moto K., et al.
  • Organizer: 9th European congress of entomology
  • Place of Presentation: Budapest, Hungary
  • Year and Date: 2010-08-26
• [Presentation] Molecular analysis of sex pheromone production-related gene in the silkmoth, Bombyx mori. (2010)
  • Author(s): Moto K., Matsumoto S.
  • Organizer: 9th European congress of entomology.
  • Place of Presentation: Budapest, Hungary
  • Year and Date: 2010-08-25
• [Presentation] アワヨトウ性フェロモン生合成に関わるアシル基還元酵素遺伝子の単離 (2010)
  • Author(s): 本賢一、李載みん、栗原政明、永田宏次、田之倉優、長澤寛道、松本正吾
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-30
• [Presentation] アワヨトウ性フェロモン生合成に関わるアシル基還元酵素遺伝子の単離 (2010)
  • Author(s): 本賢一, 他
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-03-28
• [Presentation] 害虫の繁殖抑制に応用可能なリガンドと受容体膜タンパク質の機能解析:フェロモン産生関連分子の遺伝子および機能解析 (2010)
  • Author(s): 李載〓、本賢一、大西敦、永峰俊弘、田之倉優、長澤寛道、永田宏次、松本正吾
  • Organizer: [平成22年度]ターゲットタンパク研究プログラムから見える未来3
  • Place of Presentation: 東京
  • Year and Date: 2010-03-05
• [Presentation] カイコガ性フェロモン生合成活性化神経ペプチド(PBAN)の活性型立体構造の解析 (2009)
  • Author(s): 永田宏次、岡田晃季、河合岳志、大塚淳、Jimmy Joe Hull、本賢一、松本正吾、長澤寛道、田之倉優
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2009-05-22
• [Presentation] アワヨトウ性フェロモン生合成に関わる脂肪酸不飽和化酵素 (2009)
  • Author(s): 本賢一、栗原政明、李載〓、永田宏次、長澤寛道、松本正吾
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] カイコガPBAN受容体(PBANR)アイソフォームの同定とその遺伝子発現解析 (2009)
  • Author(s): 李載〓、Jimmy Joe Hull、本賢一、大西敦、永田宏次、長澤寛道、松本正吾
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] カイコガ性フェロモン生合成活性化神経ペプチド(PBAN)の環状アゴニストのNMR構造解析 (2009)
  • Author(s): 永田宏次、岡田晃季、河合岳志、大塚淳、Jimmy Joe Hull、本賢一、松本正吾、長澤寛道、田之倉優
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] 害虫の繁殖抑制に応用可能なリガンドと受容体膜タンパク質の構造・機能解析-ガ類昆虫の性フェロモン産生関連遺伝子の単離と機能解析- (2009)
  • Author(s): 本賢一、李載〓、大西敦、Jimmy Joe Hull、長澤寛道、永田宏次、松本正吾
  • Organizer: [平成20年度]ターゲットタンパク研究プログラムから見える未来2
  • Place of Presentation: 東京
  • Year and Date: 2009-01-15
• [Book] 蛾類昆虫の性フェロモンとその生合成メカニズム (2010)
  • Author(s): 本賢一、松本正吾
  • Publisher: ビオフィリア
• [Remarks] ホームページ
  • URL: http://www.riken.jp/insect/