Structure and functions of a methanotrophic microbial food chain

• Project/Area Number: 20580061
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Nagoya University
• Principal Investigator: MURASE Jun Nagoya University, 生命農学研究科, 講師 (30285241)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 水田土壌 / メタン酸化細菌 / 原生動物 / 微生物食物連鎖 / Stable Isotope Probing / メタン酸化 / ミクロコズム / 捕食 / アメーバ / Heterolobosea

Research Abstract

In a wetland rice field, methane oxidation is active at the oxic-anoxic boundary layer, which plays a crucial role controlling methane emission to the atmosphere. The present study using stable isotope probing approach demonstrated that methanotrophic biomass is immediately grazed by soil protozoa. Soil protozoa including novel amoeba isolated from a rice field soil grazed on methanotrophic bacteria with preferential selection on a certain group. Our results suggested that protozoa is one of the most important biological factors controlling methanotrophic biomass.

Research Products

• [Journal Article] Strain-specific incorporation of methanotrophic biomass into eukaryotic grazers in a rice field soil revealed by PLFA-SIP. (2011)
  • Author(s): Murase J., Hordijk C., Tayasu I., Bodelier P.L.E.
  • Journal Title: FEMS Microbiol.Ecol. 75 Pages: 284-290
  • Peer Reviewed
• [Journal Article] Strain-specific incorporation of methanotrophic biomass into eukaryotic grazers in a rice field soil revealed by PLFA-SIP (2011)
  • Author(s): Murase J, Hordijk C, Tayasu I, Bodelier PLE
  • Journal Title: FEMS Microbiol.Ecol. Volume: 75 Pages: 284-290
  • Peer Reviewed
• [Journal Article] Isolation of a new heterolobosean amoeba from a rice field soil : Vrihiamoeba italica gen. (2010)
  • Author(s): Murase J., Kawasaki M., De Jonckheere J.F.
  • Journal Title: nov., sp.nov.Eur.J.Protistol. 46 Pages: 164-170
  • Peer Reviewed
• [Journal Article] Isolation of a new heterolobosean amoeba from a rice field soil : Vrihiamoeba italica gen.nov., sp.nov. (2010)
  • Author(s): Murase J, Kawasaki M, De Jonckheere JF
  • Journal Title: Eur.J.Protistol. Volume: 46 Pages: 164-170
  • Peer Reviewed
• [Journal Article] Low diversity of T4-type bacteriophages in applied rice straw, plant residues and rice roots in Japanese rice soils : Estimation from major capsid gene (g23) composition. (2010)
  • Author(s): Fujihara S, Murase J, Tun CC, Matsuyama T, Ikenaga M, Asakawa S, Kimura M
  • Journal Title: Soil Sci.Plant Nutr. Volume: 56 Pages: 800-812
  • NAID: 110008144377
  • Peer Reviewed
• [Journal Article] Unique viral capsid assembly protein gene (g20) of cyanophages in the floodwater of a Japanese paddy field. (2010)
  • Author(s): Wang GH, Murase J, Asakawa S, Kimura M
  • Journal Title: Biol.Fert.Soils Volume: 46 Pages: 93-102
  • Peer Reviewed
• [Journal Article] Eukaryotic communities associated with the decomposition of rice straw compost in a Japanese rice paddy field estimated by DGGE analysis. (2008)
  • Author(s): Hatamoto, M., Tanahashi, T, Murase J., Matsuya, K., Hayashi, M., Kimura, M., Asakawa, S.
  • Journal Title: Biol.Fert.Soils 44 Pages: 527-532
  • Peer Reviewed
• [Journal Article] Selective grazing of methanotrophs by protozoa in a rice field soil. (2008)
  • Author(s): Murase, J., Frenzel, P.
  • Journal Title: FEMS Microbiol.Ecol. 65 Pages: 408-414
  • Peer Reviewed
• [Journal Article] Selective grazing of methanotrophs by protozoa in a rice field soil. (2008)
  • Author(s): Murase, J., Frenzel, P.
  • Journal Title: FEMS Microbiol. Ecol. 65 Pages: 408-414
  • Peer Reviewed
• [Presentation] 微生物食物連鎖の分子生物学的解析 : メタン酸化を例として (2010)
  • Author(s): 村瀬潤
  • Organizer: 日本生態学会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-18
• [Presentation] 微生物食物連鎖の分子生物学的解析 (2010)
  • Author(s): 村瀬潤
  • Organizer: メタン酸化を例として第57回日本生態学会
  • Place of Presentation: 招待
• [Presentation] PLFA-SIP法を用いた水田土壌の原生動物によるメタン酸化細菌の捕食解析 (2010)
  • Author(s): 村瀬潤,Paul L.E. Bodelier
  • Organizer: 日本土壌微生物学会
  • Place of Presentation: 東京
• [Presentation] A methane-driven microbial food web in a rice field soil (2010)
  • Author(s): Jun Murase, Peter Frenzel
  • Organizer: 19th World Congress of Soil Science
  • Place of Presentation: メルボルン
• [Presentation] メタンを酸化している水田土壌の細菌群集に及ぼす原生動物の影響 (2010)
  • Author(s): 村瀬潤, Peter Frenzel
  • Organizer: 日本微生物生態学会
  • Place of Presentation: つくば
• [Presentation] 水田土壌の微生物食物連鎖 (2009)
  • Author(s): 村瀬潤
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 広島
  • Year and Date: 2009-11-20
• [Presentation] Impact of protozoan grazing on the community of methanotrophic bacteria in a wetland rice field soil. (2009)
  • Author(s): Murase, J., Frenzel, P.
  • Organizer: The 10^<th> International symposium on bacterial genetics and ecology
  • Place of Presentation: ウプサラ、スウェーデン
  • Year and Date: 2009-06-16
• [Presentation] Impact of protozoan grazing on the community of methanotrophic bacteria in a wetland rice field soil. (2009)
  • Author(s): Murase,J, Frenzel,P.
  • Organizer: The 10th International symposium on bacterial genetics and ecology (BAGECO-10)
  • Place of Presentation: Uppsala, Sweden
• [Presentation] 水田土壌生態系の機能における細菌-原生生物系の役割 (2008)
  • Author(s): 村瀬潤
  • Organizer: 日本土壌肥料学会2008年度愛知大会シンポジウム
  • Place of Presentation: 名古屋(招待)
  • Year and Date: 2008-09-13
• [Presentation] 原生動物が水田土壌のメタン酸化に及ぼす影響 (2008)
  • Author(s): 村瀬潤
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-09-11
• [Presentation] 原生動物が水田土壌のメタン酸化に及ぼす影響 (2008)
  • Author(s): 村瀬潤, Frenzel, P.
  • Organizer: 日本土壌微生物学会
  • Place of Presentation: 静岡
  • Year and Date: 2008-06-13
• [Book] 12.窒素サイクルメ(シリーズ現代の生態学11巻)(タンサイクルに果たす微生物の役割) (2011)
  • Author(s): 村瀬潤
  • Publisher: 共立出版
• [Book] 7.集水域の中の水田(身近な水の環境科学-源流から干潟まで-)(日本陸水学会東海支部会編) (2010)
  • Author(s): 村瀬潤
  • Publisher: 朝倉書店
• [Book] 7.水田の土壌環境と微生物相(身近な自然の保全生態学)(根本正之編著) (2010)
  • Author(s): 村瀬潤・浅川晋
  • Publisher: 培風館
• [Book] 身近な自然の保全生態学 (2010)
  • Author(s): 村瀬潤・浅川晋
  • Total Pages: 213
  • Publisher: 培風館
• [Book] 1-I3.4アメーバ、鞭毛虫(土壌の原生生物・線虫群集-その土壌生態系での役割-)(日本土壌肥料学会編) (2009)
  • Author(s): 村瀬潤
  • Publisher: 博友社
• [Book] 2-III.水田土壌生態系の機能における細菌-原生生物系の役割(土壌の原生生物・線虫群集-その土壌生態系での役割-)(日本土壌肥料学会編) (2009)
  • Author(s): 村瀬潤
  • Publisher: 博友社
• [Book] 土壌の原生生物・線虫群集-その土壌生態系での役割- (2009)
  • Author(s): 村瀬潤(分担執筆)
  • Total Pages: 172
  • Publisher: 博友社
• [Remarks] ホームページ等