Identification and analysis of rice and barley aquaporins as arsenite transporters.

• Project/Area Number: 20580063
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Okayama University
• Principal Investigator: KATSUHARA Maki Okayama University, 資源植物科学研究所, 准教授 (00211847)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: アクアポリン / 亜ヒ酸輸送系 / イネ / オオムギ / 土壌汚染 / 酵母 / 物質輸送 / 亜ヒ酸 / 酵母スクリーニング系 / 輸送系 / ヒ素汚染 / ヒ素高感受性変異酵母 / ヒ素低吸収・排出型作物 / 輸送体

Research Abstract

In this project, rice and barley aquaporins were selected and analyzed form the view to elucidate the molecular mechanism of arsenite transports in Poaceae plants. Final goal of this project is to breed rice/barley lines containing low As. Using screening system with yeast cells, 5 aquaporin genes (including known 3 genes) were identified as arsenite transporters. Among these genes, temporal increase of OsNIP2 ; 1 expression in roots and decrease of OsNIP3;3 in leaves were observed in rice plants treated with arsenite.

Research Products

• [Journal Article] Expanding roles of plant aquaporins in plasma membranes and cell organelles. (2008)
  • Author(s): Katsuhara, M., Hanba, T.Y., Shiratake, K., Maeshima, M.
  • Journal Title: Functional Plant Biology 35 Pages: 1-14
• [Presentation] Detection of transport activity in barley and rice aquaporins with newly developed yeast system. (2011)
  • Author(s): 李志?, 森泉, 篠野静香, 且原真木
  • Organizer: 日本植物生理学会2011年度年会(仙台)
  • Place of Presentation: 東日本大震災のため東北大学での年会は中止されたが、要旨集の公開を持って成立となった
  • Year and Date: 2011-03-20
• [Presentation] Water transport in plants under stresses : from molecules to whole plant. (2010)
  • Author(s): M.Katsuhara
  • Organizer: The 5th JKUAT Scientific, Technological and Industrialisation Conference
  • Place of Presentation: Nairobi, Kenya
• [Book] 植物栄養学(第2版のうち「水の吸収と輸送」)(間藤徹、馬建鋒、藤原徹編、総ページ288ページ) (2010)
  • Author(s): 且原真木
  • Publisher: 文永堂
• [Book] 水の吸収と輸送 (「植物栄養学(第2版)」間藤徹、馬建鋒、藤原徹編) (2010)
  • Author(s): 且原真木
  • Publisher: 文永堂