| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Selenium (Se) has been recognized as an essential trace element for animal and human because it is an integral part of the enzyme glutathione peroxidase, a seleno enzyme that prevents oxidative damage to cells. In contrast, higher plants do not require Se, and the question of whether Se is an essential element to plants remains controversial. Previous reports suggest that trace amounts of Se may serve a role in antioxidative mechanisms and partially reduce the adverse effects of cadmium on the growth. The aim of this work was to investigate novel physiological function of Se and Se-dependent regulatory factor in Arabidopsis thaliana seedlings. The influence of Se (sodium selenate) supplementation in the range of 0.1-100 μM on the biomass of Arabidopsis plants was studied 18 days after sowing. A significant decrease in the shoot fresh weight of Arabidopsis seedlings was noted at greater than 50 μM. However, the fresh weight of the seedlings grown in the medium with 0.5-5 μM Se significantly increased over that of seedlings grown without Se. There was significant effect of Se supplementation on the ATP content and respiration, as compared with control plants. In Arabidopsis seedlings grown in the medium with 1 μM Se, NAD dependent glyceraldehyde-3-phosphate dehydrogenase and triosephosphate isomerase activities were 1.5-fold higher than those in seedlings grown without Se.
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