Two-component membrane pore-forming toxins - The elucidation of the details of cytolysis mechanism, and its application to the cell-specific toxin molecular design-
| Project/Area Number |
20580069
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Tohoku University |
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Principal Investigator |
KANEKO JUN Tohoku University, 大学院・農学研究科, 准教授 (30221188)
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| Co-Investigator(Kenkyū-buntansha) |
神尾 好是 尚絅学院大学, 健康栄養学科, 教授 (00109175)
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| Co-Investigator(Renkei-kenkyūsha) |
KAMIO Yoshiyuki 東北学院大学, 環境防災工学研究所, 客員教授 (00109175)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 微生物機能 / 2成分性膜孔形成毒素 / Staphylococcus aureus / 二成分性毒素 / 病原性 / 標的細胞 / 応答 / Staphylococous aureus / 二成合性毒素 |
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| Research Abstract |
Staphylococcal γ-hemolysin and leukocidins are two-component hetero oligomeric pore-forming cytolytic toxins. In this study, the mode of action including cell specificity and horizontal gene transfer system of these toxins were investigated.
1) Receptors for the initial components on the target cells were explored.
2) The intra-molecular factor(s) involved in the opening pore was searched.
3) The mechanism causing the white cell lysis and inflammation was studied.
4) Novel basteriophages related to horizontal transfer of Panton Valentine leukosidin was characterized, and the phage protein which recognize host cell was identified.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Toll-like receptor 4-dependent activation of myeloid dendritic cells by leukocidin of Staphylococcus aureus2009
Author(s)
K.Inden, J.Kaneko, A.Miyazato, N.Yamamoto, S.Mouri, Y.Shibuya, K.Nakamura, T.Aoyagi, M.Hatta, H.Kunishima, Y.Hirakata, Y.Itoh, M.Kaku, K.Kawakami
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Journal Title
Microbes Infect. 11(2)
Pages: 245-253
Related Report
Peer Reviewed
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