Structural and functional analyses of xylan-degrading esterases from koji-mold

• Project/Area Number: 20580071
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Yamagata University
• Principal Investigator: KOSEKI Takuya Yamagata University, 農学部, 准教授 (70372191)
• Co-Investigator(Renkei-kenkyūsha): FUSHINOBU Shinya 東京大学, 大学院・農学生命科学研究科, 助教 (00302589)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 植物バイオマス / ヘミセルロース / フェルラ酸エステラーゼ / 麹菌 / Aspergillus oryzae / タンナーゼ / パラベンエステラーゼ / アラビノース結合モジュール / Aspergillus awamori / Aspergillus kawachii / フェルラ酸

Research Abstract

In this study we report the relationship between structure and function of the esterases involved in the degradation of plant cell wall. Based on the Aspergillus oryzae genomic database, we characterized a novel ferulic acid esterase and a chimeric enzyme comprising ferulic acid esterase and family 42 carbohydrate-binding module. Moreover, we characterized a novel A. oryzae esterase that hydrolyzes 4-hydroxybenzoic acid esters.

Research Products

• [Journal Article] A novel Aspergillus oryzae esterase that hydrolyzes 4-hydroxybenzoic acid esters (2010)
  • Author(s): Koseki T., Mihara K., Murayama T., Shiono Y.
  • Journal Title: FEBS Lett. 584 Pages: 4032-4036
  • Peer Reviewed
• [Journal Article] Expression of feruloyl esterase from Aspergillus awamori in Escherichia coli : Characterization and crystal studies of the recombinant enzyme. (2010)
  • Author(s): Fazary A.E., Hamad H.A., Lee J.C., Koseki T., Lee C.K., Ju Y.H.
  • Journal Title: Int.J.Biol.Macromol. 46 Pages: 440-444
  • Peer Reviewed
• [Journal Article] a new α-pyron metabolite with potent cytotoxicity from an endophytic fungus Allantophomopsis lycopodina KS-97. (2010)
  • Author(s): Shiono Y., Yokoi M., Koseki T., Murayama T., Aburai N., Kimura K., Allantopyron A
  • Journal Title: J.Antibiot. 63 Pages: 251-253
  • Peer Reviewed
• [Journal Article] Eremophilane sesquiterpenes from the endophyte Microdiplodia sp.KS 75-1 and revision of the stereochemistries of phomadecalins C and D. (2010)
  • Author(s): Hatakeyama, T., Koseki, T., Murayama, T., Shiono, Y.
  • Journal Title: Phytochemistry Lett. 3 Pages: 148-151
  • Peer Reviewed
• [Journal Article] Anti-metabolic syndrome effects of adenosine ingestion in stroke-prone spontaneously hypertensive rats fed a high fat diet. (2010)
  • Author(s): Ardiansyah, Shirakawa, H., Sugita, Y., Koseki, T., Komai, M.
  • Journal Title: Br.J.Nutr. 46 Pages: 440-444
  • Peer Reviewed
• [Journal Article] Characterization of a chimeric enzyme comprising feruloyl esterase and family 42 carbohydrate-binding module. (2010)
  • Author(s): Koseki T., Mochizuki K., KisaraH., Miyanaga A., Fushinobu S., Murayama T., Shiono Y.
  • Journal Title: Appl.Microbiol.Biotechnol. 86 Pages: 155-161
  • Peer Reviewed
• [Journal Article] A novel Aspergillus oryzae esterase that hydrolyzes 4-hydroxybenzoic acid esters (2010)
  • Author(s): KOSEKI, T., MIHARA.K., MURAYAMA, T., SHIONO, Y.
  • Journal Title: FEBS Letters Volume: 584 Pages: 4032-4036
  • Peer Reviewed
• [Journal Article] Expression of feruloyl esterase from Aspergillus awamori in Escherichia coli : Characterization and crystal studies of the recombinant enzyme (2010)
  • Author(s): FAZARY.A.E., HAMAD, H.A., LEE, J.C., KOSEKI, T., LEE, C.K., JUY.H.
  • Journal Title: International Journal of Biological Macromolecule Volume: 46 Pages: 440-444
  • Peer Reviewed
• [Journal Article] AllantopyronA, a new a-pyron metabolite with potent cytotoxicity from an endop hytic fungus Allantophomopsis lycopodina KS-97 (2010)
  • Author(s): SHIONO, Y., YOKOI, M., KOSEKI, T., MURAYAMA, T., ABURAI, N., KIMURA, K.
  • Journal Title: The Journal of Antibiotics Volume: 63 Pages: 251-253
  • Peer Reviewed
• [Journal Article] Eremophilane sesquiterpenes from the endophyte Microdiplodia sp.KS 75-1 and revision of the stereochemistries of phomadecalins C and D (2010)
  • Author(s): HATAKEYAMA, T., KOSEKI, T., MURAYAMA, T., SHIONO, Y.
  • Journal Title: Phytochemistry Letters Volume: 3 Pages: 148-151
  • Peer Reviewed
• [Journal Article] Anti-metabolic syndrome effects of adenosine ingestion in stroke-prone spontaneously hypertensive rats fed a high fat diet (2010)
  • Author(s): ARDIANSYAH, SHIRAKAWA, H., SUGITA, Y., KOSEKI, T., KOMAI, M.
  • Journal Title: British Journal of Nutrition Volume: 46 Pages: 440-444
  • Peer Reviewed
• [Journal Article] Characterization of a chimeric enzyme comprising feruloyl esterase and family 42 carbohydrate-binding module (2010)
  • Author(s): Koseki T., Mochizuki K., Kisara H., Miyanaga A., Fushinobu S., Murayama T., Shiono Y.
  • Journal Title: Applied Microbiolgy and Biotechnology 86 Pages: 155-161
  • Peer Reviewed
• [Journal Article] Occurrence, properties, and applications of feruloyl esterases. (2009)
  • Author(s): Koseki T., Fushinobu S., Ardiansyah, Shirakawa H., Komai M.
  • Journal Title: Appl.Microbiol.Biotechnol. 84 Pages: 803-810
  • Peer Reviewed
• [Journal Article] Characterization of two distinct feruloyl esterases, AoFaeB and AoFaeC, from Aspergillus oryzae. (2009)
  • Author(s): Koseki T., Hori A., Seki S., Murayama T., Shiono Y.
  • Journal Title: Appl.Microbiol.Biotechnol. 83 Pages: 689-696
  • Peer Reviewed
• [Journal Article] A new benzoxepin metabolite isolated from endophytic fungus Phomopsis sp. (2009)
  • Author(s): Shiono Y., Nitto A., Shimanuki K., Koseki T., Murayama T., Miyakawa T., Yoshida, J., Kimura K.
  • Journal Title: J.Antibiot. 62 Pages: 533-535
  • NAID: 10030636414
  • Peer Reviewed
• [Journal Article] Occurrence, properties, and applications of feruloyl esterases (2009)
  • Author(s): Koseki T., Fushinobu S., Ardiansyah, Shirakawa H., Komai M.
  • Journal Title: Applied Microbiolgy and Biotechnology 84 Pages: 803-810
  • Peer Reviewed
• [Journal Article] Characterization of two distinct feruloyl esterases, AoFaeB and AoFaeC, from Aspergillus oryzae (2009)
  • Author(s): T. Koseki, A. Hori, S. Seki, T. Murayama, Y. Shiono
  • Journal Title: Applied Microbiology and Biotechnology (in press)
  • Peer Reviewed
• [Journal Article] Characterization of an α-L-rhamnosidase from Aspergillus kawachii and its gene. (2008)
  • Author(s): Koseki T., Mese Y., Nishibori N., Masaki K., Fujii T., Handa T., Yamane Y., Shiono Y., Murayama T., Iefuji H.
  • Journal Title: Appl.Microbiol.Biotechnol. 80 Pages: 1007-1013
  • Peer Reviewed
• [Journal Article] Novel effects of a single administration of ferulic acid on the regulation of blood pressure and hepatic lipid metabolic profile in stroke-prone spontaneously hypertensive rats. (2008)
  • Author(s): Ardiansyah, Ohsaki Y., Shirakawa H., Koseki T., Komai M.
  • Journal Title: J.Agric.Food Chem. 56 Pages: 2825-2830
  • Peer Reviewed
• [Journal Article] Pyrrospirones A and B, apoptosis inducers in HL-60 cells, from an endophytic fungus, Neonectria ramulariae Wollenw KS-246. (2008)
  • Author(s): Shiono Y., Shimanuki K., Hiramatsu F., Koseki T., Murayama T., Fujisawa N., Kimura K.
  • Journal Title: Bioorg.Med.Chem.Lett. 18 Pages: 6050-6053
  • Peer Reviewed
• [Journal Article] Characterization of an α-L-rhamnosidase from Aspergillus kawachii and itsgene (2008)
  • Author(s): T. Koseki, Y. Mese, N. Nishibori, K. Masaki, T. Fujii, T. Handa, Y. Yamane, Y. Shiono, T. Murayama, and H. Iefuji
  • Journal Title: Applied Microbiology and Biotechnology 80 Pages: 1007-1013
  • Peer Reviewed
• [Presentation] 子嚢菌類Microdiplodia sp.KS-75-1株の生産するテトロニック酸関連物質について (2011)
  • Author(s): 畠山泰二, 小関卓也, 村山哲也, 塩野義人
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大,京都市
  • Year and Date: 2011-03-27
• [Presentation] Aspergillus oryzae RIB40由来フェルラ酸エステラーゼのユニークなジスルフィド結合の役割 (2011)
  • Author(s): 河本かずさ,鈴木健太郎,伏信進矢,若木高善,村山哲也,塩野義人,小関卓也
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都市
  • Year and Date: 2011-03-26
• [Presentation] Aspergillus oryzae RIB40由来新規パラベンエステラーゼ(AoPrbA)の特徴付け (2011)
  • Author(s): 小関卓也,見原好治,村山哲也,塩野義人
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都市
  • Year and Date: 2011-03-26
• [Presentation] Aspergillus oryzae RIB40由来新規パラベンェステラーゼ(AoPrbA)の特徴付け (2011)
  • Author(s): 小関卓也, 見原好治, 村山哲也, 塩野義人
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大,京都市
  • Year and Date: 2011-03-26
• [Presentation] Aspergillus oryzae RIB40由来フェルラ酸エステラーゼのユニークなジスルフィド結合の役割 (2011)
  • Author(s): 河本かずさ, 鈴木健太郎, 伏信進矢, 若木高善, 村山哲也, 塩野義人, 小関卓也
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大,京都市
  • Year and Date: 2011-03-26
• [Presentation] 低温性糸状菌Penicillium antarcticum FH-14株の生産する桂皮酸エステラーゼの精製と性質 (2010)
  • Author(s): 田巻大輔,村山哲也,塩野義人,小関卓也
  • Organizer: 第62回日本生物工学会大会
  • Place of Presentation: 宮崎市
  • Year and Date: 2010-10-29
• [Presentation] 低温性糸状菌Penicillium anfareticum FH-14株の生産する桂皮酸エステラーゼの精製と性質 (2010)
  • Author(s): 田大輔, 村山哲也, 塩野義人, 小関卓也
  • Organizer: 第62回日本生物工学会大会
  • Place of Presentation: ワールドコンベンションセンターサミット,宮崎市
  • Year and Date: 2010-10-29
• [Presentation] クロサイワイタケ科の子実体から分離した糸状菌の生産する物質について (2010)
  • Author(s): 早坂信吾, 小関卓也, 村山哲也, 塩野義人
  • Organizer: 日本農芸化学会東北支部・北海道支部合同支部大会
  • Place of Presentation: 東北大学農学部,仙台市
  • Year and Date: 2010-09-28
• [Presentation] アデノシン、AMPの長期経口投与は高脂肪食給餌肥満モデルマウスの糖代謝異常を改善する (2010)
  • Author(s): 稲川裕人, Ardiansyah, 白川仁, 小関卓也, 駒井三千夫
  • Organizer: 日本農芸化学会東北支部・北海道支部合同支部大会
  • Place of Presentation: 東北大学農学部,仙台市
  • Year and Date: 2010-09-28
• [Presentation] Feruloyl esterases : potential in grain processing (2010)
  • Author(s): Koseki, T.
  • Organizer: BIT's 1st Inaugural Symposium on Enzymes & Biocatalysis-2010
  • Place of Presentation: 上海(招待講演)
  • Year and Date: 2010-04-23
• [Presentation] Feruloyl esterases : potential in grain processing (2010)
  • Author(s): Koseki.T
  • Organizer: BIT's 1st Inaugural Symposium on Enzymes & Biocatalysis-2010
  • Place of Presentation: 上海,中国(招待講演)
  • Year and Date: 2010-04-23
• [Presentation] 麹菌由来フェルラ酸エステラーゼのX 線結晶構造解析 (2010)
  • Author(s): 鈴木健太郎,小関卓也,小宮大,伏信進矢,祥雲弘文,若木高善
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京都
  • Year and Date: 2010-03-28
• [Presentation] Polyketides and epoxydon derivatives from endophytic fungus Hypoxylon polyporus (2010)
  • Author(s): POUMALE H.M., KOSEKI T., MURAYAMA T., FUJISAWA N., KIMURA K., SHIONO Y.
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-28
• [Presentation] 麹菌由来フェルラ酸エステラーゼのX線結晶構造解析 (2010)
  • Author(s): 鈴木健太郎, 小関卓也, 小宮大, 伏信進矢, 祥雲弘文, 若木高善
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-28
• [Presentation] Aspergillus oryzae由来パラベンエステル特異的加水分解酵素 (2009)
  • Author(s): 見原好治,村山哲也,塩野義人,小関卓也
  • Organizer: 第9回糸状菌分子生物学コンファレンス
  • Place of Presentation: 東京都
  • Year and Date: 2009-11-18
• [Presentation] Aspergillus oryzae由来パラベンエステル特異的加水分解酵素 (2009)
  • Author(s): 見原好治, 村山哲也, 塩野義人, 小関卓也
  • Organizer: 第9回糸状菌分子生物学コンファレンス
  • Place of Presentation: 東京
  • Year and Date: 2009-11-18
• [Presentation] 糸状菌 Microdiplodia sp.KS-75-1株から分離されたセスキテルペン類について (2009)
  • Author(s): 畠山泰二、小関卓也、村山哲也、塩野義人
  • Organizer: 日本農芸化学会東北支部第144回大会
  • Place of Presentation: 盛岡
  • Year and Date: 2009-10-31
• [Presentation] 麹菌由来フェルロイルエステラーゼとアラビノース結合モジュールとのキメラ酵素の特性 (2009)
  • Author(s): 望月敬司,宮永顕正,伏信進矢,村山哲也,塩野義人,小関卓也
  • Organizer: 日本応用糖質科学会平成21年度大会
  • Place of Presentation: 弘前市
  • Year and Date: 2009-09-16
• [Presentation] 麹菌由来フェルロイルエステラーゼとアラビノース結合モジュールとのキメラ酵素の特性 (2009)
  • Author(s): 望月敬司, 宮永顕正, 伏信進矢, 村山哲也, 塩野義人, 小関卓也
  • Organizer: 日本応用糖質科学会平成21年度大会
  • Place of Presentation: 弘前
  • Year and Date: 2009-09-16
• [Presentation] Aspergillus oryzae由来フェルラ酸エステラーゼBの結晶構造解析 (2009)
  • Author(s): 鈴木健太郎, 小関卓也, 小宮大, 伏信進矢, 祥雲弘文, 若木高善
  • Organizer: 日本農芸化学会関東支部2009年度大会
  • Place of Presentation: 東京
  • Year and Date: 2009-07-04
• [Presentation] 麹菌由来のヘミセルロース側鎖分解酵素とアラビノース結合モジュールとのキメラ酵素の特性 (2009)
  • Author(s): 小関卓也
  • Organizer: 第10回酵素応用シンポジウム
  • Place of Presentation: 名古屋市(招待講演)
  • Year and Date: 2009-06-12
• [Presentation] 麹菌由来のヘミセルロース側鎖分解酵素とアラビノース結合モジュールとのキメラ酵素の特性 (2009)
  • Author(s): 小関卓也
  • Organizer: 第10回酵素応用ジンポジウム
  • Place of Presentation: 名古屋(招待講演)
  • Year and Date: 2009-06-12
• [Presentation] Oral administration of L-tryptophan is effective to improve glucose metabolism and hypertension in stroke-prone spontaneously hypertensive rats (2009)
  • Author(s): Ardiansyah, H.Shirakawa, T.Koseki, Y.Shiono, T.Murayama, M.Komai
  • Organizer: 3rd International Congress on Prediabetes and the Metabolic Syndrome
  • Place of Presentation: Nice, France
  • Year and Date: 2009-04-22
• [Presentation] Anti-metabolic syndrome effects of adenosine in stroke-prone spontaneously hypertensive rats (2009)
  • Author(s): Ardiansyah, H.Shirakawa, T.Koseki, Y.Shiono, T.Murayama, M.Komai
  • Organizer: 3rd International Congress on Prediabetes and the Metabolic Syndrome
  • Place of Presentation: Nice, France
  • Year and Date: 2009-04-21
• [Presentation] Aspergillus oryzae由来2つのフェルロイルエステラーゼの性質 (2009)
  • Author(s): 堀茜,関翔二,塩野義人,村山哲也,小関卓也
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡市
  • Year and Date: 2009-03-29
• [Presentation] Aspergillus awamori由来アセチルキシランエステラーゼの結晶構造に基づく機能改変 (2009)
  • Author(s): 小宮大,石田卓也,小関卓也,伏信進矢,五十嵐圭日子,鮫島正浩,若木高善,祥雲弘
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡市
  • Year and Date: 2009-03-28
• [Presentation] Aspergillus awamori由来アセチルキシランエステラーゼの結晶構造解析 (2008)
  • Author(s): 小宮大、石田卓也、小関卓也、伏信進矢、五十嵐圭日子、鮫島正浩、若木高善、祥雲弘文
  • Organizer: 平成20年度日本応用糖質科学会
  • Place of Presentation: 沖縄県那覇市
  • Year and Date: 2008-09-18
• [Presentation] Identification of a type-B feruloyl esterase from Aspergillus oryzae (2008)
  • Author(s): T. Koseki, A. Hori, T. Murayama, Y. Shiono
  • Organizer: Mie Bioforum 2008
  • Place of Presentation: Shima, Mie
  • Year and Date: 2008-09-03
• [Presentation] 焼酎麹菌の植物バイオマス分解酵素の特性とその利用 (2008)
  • Author(s): 小関卓也
  • Organizer: 平成20年度日本生物工学会大会シンポジウム
  • Place of Presentation: 仙台市(招待講演)
  • Year and Date: 2008-08-27
• [Presentation] 焼酎麹菌の植物バイオマス分解酵素の特性とその利用 (2008)
  • Author(s): 小関卓也
  • Organizer: 平成20年度日本生物工学会大会シンポジウム
  • Place of Presentation: 宮城県仙台市
  • Year and Date: 2008-08-27
• [Presentation] 焼酎麹菌の植物バイオマス分解酵素の特性 (2008)
  • Author(s): 小関卓也
  • Organizer: 日本農芸化学会東北支部シンポジウム
  • Place of Presentation: 山形県鶴岡市
  • Year and Date: 2008-07-12
• [Presentation] Identification of a type-B feruloyl esterase from Aspergillus oryzae (2008)
  • Author(s): Koseki T., Hori A., Murayana T., Shiono Y.
  • Organizer: 5th European Symposium on Enzymes in Grain Processing
  • Place of Presentation: Norwich,英国
  • Year and Date: 2008-04-01
• [Presentation] Identification of a type-B feruloyl esterase from Aspergillus oryzae (2008)
  • Author(s): T. Koseki, A. Hori, T. Murayama, Y. Shiono
  • Organizer: 5th European Symposium on Enzymes in Grain Processing
  • Place of Presentation: Norwich, UK
  • Year and Date: 2008-04-01
• [Book] 分子麹菌学(北本勝ひこ編)麹菌由来ヘミセルラーゼの構造と機能 (2011)
  • Author(s): 小関卓也,伏信進矢
  • Publisher: 日本醸造協会(印刷中)
• [Remarks] ホームページ等
  • URL: http://www.tr.yamagata-u.ac.jp/~yshiono/
• [Remarks]
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• [Remarks]
  • URL: http://www.tr.yamagata-u.ac.jp/~yshiono/
• [Remarks]
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