Molecular mechanisms of the maintenance of sterol homeostasis in yeast

• Project/Area Number: 20580072
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: The University of Tokyo
• Principal Investigator: FUKUDA Ryouichi 東京大学, 大学院・農学生命科学研究科, 助教 (50323481)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 酵母 / ステロール / 脂質 / 輸送 / ホメオスタシス

Research Abstract

We constructed a system, in which the transport of sterol from the plasma membrane to the ER is evaluated in vitro using budding yeast Saccharomyces cerevisiae, and analyzed its mechanism. We also construct systems to assay the sterol transport from the ER to the vacuole or mitochondria in yeast.

Research Products

• [Journal Article] Incorporation and remodeling of phosphatidylethanolamine containing short acyl residues in yeast (2010)
  • Author(s): L. Deng, R. Fukuda, T. Kakihara, K. Narita, and A. Ohta
  • Journal Title: Biochim. Biophys. Acta Volume: 1801 Pages: 635-645
• [Journal Article] Incorporation and remodeling of phosphatidylethanolamine containing short acylresidues in yeast (2010)
  • Author(s): Lan.Deng, Ryouichi. Fukuda, Toru Kakihara, Koichi Narita, Akinori Ohta
  • Journal Title: Biochim. Biophys. Acta Volume: 1801 Pages: 635-645
  • Peer Reviewed
• [Journal Article] Involvement of LEM3/ROS3 in the uptake of phosphatidylcholine with short acyl chains in Saccharomyces cerevisiae (2009)
  • Author(s): Y. Ono, R. Fukuda, and A. Ohta
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 73 Pages: 750-752
• [Journal Article] Involvement of LEM3/ROS3 in the uptake of phosphatidylcholine with short acyl chains in Saccharomyces cerevisiae. (2009)
  • Author(s): Y.Ono, R.Fukuda, A.Ohta
  • Journal Title: Biosci.Biotechnol.Biochem. 73 Pages: 750-752
  • Peer Reviewed
• [Journal Article] Manipulation of major membrane lipid synthesis and its effects on sporulation in Saccharomyces cerevisiae (2008)
  • Author(s): L. Deng, J. Nagasawa, Y. Ono, Y. Ishikawa, T. Kakihara, R. Fukuda, and A. Ohta
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 72 Pages: 2362-2368
  • Peer Reviewed
• [Journal Article] Manipulation of major membrane lipid synthesis and its effects on sporulation in Saccharomvces cerevisiae. (2008)
  • Author(s): L. Deng, J. Nagasawa, Y. Ono, Y. Ishikawa, T. Kakihara, R. Fukuda, and A. Ohta
  • Journal Title: Biosci. Biotechnol. Biochem. 72 Pages: 2219-2223
  • Peer Reviewed
• [Presentation] ホスファチジルエタノールアミンのオルガネラ間輸送機構の解析 (2010)
  • Author(s): 小林新吾、福田良一、太田明徳
  • Organizer: 酵母遺伝学フォーラム
  • Place of Presentation: 奈良
  • Year and Date: 2010-09-09
• [Presentation] ホスファチジルエタノールアミンのオルガネラ間輸送機構の解析 (2010)
  • Author(s): 小林新吾、福田良一、太田明徳
  • Organizer: 酵母遺伝学フォーラム
  • Place of Presentation: ならまちセンター(奈良)
  • Year and Date: 2010-09-09
• [Presentation] 出芽酵母におけるホスファチジルエタノールアミンのオルガネラ間輸送機構の解析 (2010)
  • Author(s): 小林新吾、福田良一、太田明徳
  • Organizer: 日本農芸化学会大会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] 出芽酵母におけるホスファチジルエタノールアミンのオルガネラ間輸送機構の解析 (2010)
  • Author(s): 小林新吾、福田良一、太田明徳
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] 出芽酵母の胞子形成における脂質の役割 (2008)
  • Author(s): Lan Deng、長澤順平、福田良一、太田明徳
  • Organizer: 日本農芸化学会関東支部2008年度大会
  • Place of Presentation: 甲府
  • Year and Date: 2008-10-11