Studies on the physiological functions of phospholipases A2

• Project/Area Number: 20580074
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: The University of Tokyo
• Principal Investigator: ARIOKA Manabu 東京大学, 大学院・農学生命科学研究科, 准教授 (20242159)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 微生物代謝 / ホスホリパーゼA2 / リゾリン脂質 / MAPキナーゼ / ミトコンドリア / 麹菌 / PC12

Research Abstract

In PC12 cells, nerve growth factor(NGF) activates MAPK phosphorylation and induces neuronal differentiation, which is potently enhanced by lysophosphatidylcholine(LPC). No other lysophospholipids tested displayed similar activity. MAPK activation/phosphorylation that occurs downstream of other growth factors, such as epidermal and fibroblast growth factors, was not affected by LPC. TrkA autophosphorylation elicited by NGF was markedly enhanced by LPC, suggesting that the effect of LPC was specific to NGF-TrkA signaling pathway. AoPlaA in the filamentous fungus Aspergillus oryzae localizes to mitochondria. Subcellular fractionation experiment showed that AoPlaA localized to the intramembrane space of mitochondria. Phospholipid analysis demonstrated that phosphatidylethanolamine and cardiolipin was decreased and increased in AoplaA-overexpressing and-deleted strains, respectively.

Research Products

• [Journal Article] Distinct enzymatic and cellular characteristics of two secretory phospholipases A2 in the filamentous fungus Aspergillus oryzae (2009)
  • Author(s): Nakahama, T., Nakanishi, Y., Viscomi, A. R., Takaya, K., Kitamoto, K., Ottonello, S., and Arioka, M
  • Journal Title: Fungal Genet. Biol Volume: 47 Pages: 318-331
  • Peer Reviewed
• [Journal Article] A cytosolic phospholipase A2-like protein in the filamentous fungus Aspergillus oryzae localizes to the intramembrane space of the mitochondria (2009)
  • Author(s): Takaya, K., Higuchi, Y., Kitamoto, K., and Arioka, M.
  • Journal Title: FEMS Microbiol. Lett Volume: 301 Pages: 201-219
  • Peer Reviewed
• [Journal Article] Lysophosphatidylcholine protects cerebellar granule neurons from apoptotic cell death (2009)
  • Author(s): Ikeno, Y., Cheon, S. H., Konno, N., Nakamura, A., Kitamoto, K., and Arioka, M.
  • Journal Title: J. Neurosci. Res Volume: 87 Pages: 190-199
  • Peer Reviewed
• [Journal Article] A cytosolic phospholipase A_2-like protein in the filamentous fungus Aspergillus oryzae localizes to the intramembrane space of the mitochondria (2009)
  • Author(s): Takaya, K., Higuchi, Y., Kitamoto, K., Arioka, M.
  • Journal Title: FEMS Microbiol.Lett. 301 Pages: 201-219
  • Peer Reviewed
• [Journal Article] Distinct enzymatic and cellular characteristics of two secretory phospholipases A_2 in the filamentous fungus Aspergillus oryzae (2009)
  • Author(s): Nakahama, T., Nakanishi, Y., Viscomi, A.R., Takaya, K., Kitamoto, K., Ottonello, S., Arioka, M.
  • Journal Title: Fungal Genet.Biol. 47 Pages: 318-331
  • Peer Reviewed
• [Journal Article] Lysophosphatidylcholine protects cerebellar granule neurons from apoptotic cell death (2009)
  • Author(s): Ikeno, Y., Cheon, S.H., Konno, N., Nakamura, A., Kitamoto, K., Manabu Arioka
  • Journal Title: J.Neurosci.Res. 87 Pages: 190-199
  • Peer Reviewed
• [Presentation] Lysophosphatidylcholine enhances NGF-induced TrkA signaling in PC12 cells (2011)
  • Author(s): Wuhanqimuge, Kitamoto, K., Arioka, M
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2011-03-27
• [Presentation] 麹菌A. oryzaeの持つ細胞質型ホスホリパーゼA2の局在および機能解析 (2010)
  • Author(s): 小谷昌平、高谷康平、北本勝ひこ、有岡学
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学教養学部
  • Year and Date: 2010-03-29
• [Presentation] 麹菌A.oryzaeの持つ細胞質型ホスホリパーゼA2の局在および機能解析 (2010)
  • Author(s): 小谷昌平、高谷康平、北本勝ひこ、有岡学
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学教養学部
  • Year and Date: 2010-03-29
• [Presentation] Enhanced neurotrophic effect of nerve growth factor by lysophosphatidylcholine (2009)
  • Author(s): Itakura, A., Matsuki, Y., and Arioka, M.
  • Organizer: 4th International Conference on Phospholipase A2 and Lipid Mediators
  • Place of Presentation: Tokyo
• [Presentation] Enhanced neurotrophic effect of nerve growth factor by lysophosphatidylcholine (2009)
  • Author(s): Itakura, A., Matsuki, Y., 有岡学
  • Organizer: 4th International Conference on Phospholipase A_2 and Lipid Mediators
  • Place of Presentation: Tokyo
• [Presentation] リゾリン脂質とNGFの協調的な神経栄養因子作用のメカニズム (2008)
  • Author(s): 板倉亜沙子、北本勝ひこ、有岡学
  • Organizer: 日本農芸化学会関東支部2008年度大会
  • Place of Presentation: 山梨大学
  • Year and Date: 2008-10-11
• [Presentation] 麹菌A. oryzaeにおける細胞質型ホスホリパーゼA2の局在と機能の解析 (2008)
  • Author(s): 高谷康平、北本勝ひこ、有岡学
  • Organizer: 日本農芸化学会関東支部2008年度大会
  • Place of Presentation: 山梨大学
  • Year and Date: 2008-10-11
• [Presentation] 麹菌 A.oryzae における細胞質型ホスホリパーゼA_2の局在と機能の解析 (2008)
  • Author(s): 高谷康平、北本勝ひこ、有岡学
  • Organizer: 日本農芸化学会関東支部2008年度大会
  • Place of Presentation: 山梨大学
  • Year and Date: 2008-10-11