| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Methanol dehydrogenase (MDH) of Methylobacterium sp.MAFF211642, a methyltrophic bacterium, was remarkably increased by addition of La to methanol medium, indicating that the MDH was induced by La.Induction of MDH by La was observed in Methylobacterium spp.MDH of Bradyrhizobium sp.MAFF 211645 was also induced by Ce.Thus, La or Ce can induce MDH in methylotrophic bacteria. Furthermore, molecular structure of both MDHs was homodimer that was different to the heterotetrameric structure of Ca-dependent MDH in Methylobacterium spp.In addition La-induced MDH in Methylobacterium spp.and Ce-induced MDH in Bradyrhizobium sp.MAFF211645 were found to be encoded by xoxF and mxaF', respectively. These phenomena were also observed in well-studied M.extorquens AM1.
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