| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
We found that severe ethanol stress generates budding yeast stress granules (SGs) in a manner independent of the phosphorylation of eIF2alpha. The concentration that generated budding yeast SGs (above 10%) was higher than that causing P-bodies (more than 6%), and P-bodies were assembled prior to SGs. As well as mammalian SGs, the assembly of budding yeast SGs under ethanol stress was blocked by cycloheximide. On the other hand, the budding yeast SGs caused by ethanol stress contained eIF3c but not eIF3a and eIF3b, although the eIF3 complex is a core constituent of mammalian SGs. We also found that the formation of budding yeast SGs might play a role for sufficient recovery from ethanol stress. Additionally, we found novel phenomena about the selective export of mRNA and hyperadenylation of HSP mRNAs under ethanol stressed conditions and brewing process.
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